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Sensors 2018, 18(5), 1309;

Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection

Robert Bosch GmbH, Corporate Research, Robert-Bosch-Campus 1, D-71272 Renningen, Germany
Department of Functional Materials, University of Bayreuth, Universitätsstraße 30, D-95440 Bayreuth, Germany
Author to whom correspondence should be addressed.
Received: 12 March 2018 / Revised: 12 April 2018 / Accepted: 16 April 2018 / Published: 24 April 2018
(This article belongs to the Section Biosensors)
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The cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fluidic system with temperature control for read-out. Therefore, two sets of results are presented. First, the sensor was set up in sandwich format on single-walled carbon nanotube electrodes and was read out by applying the hydrogen peroxide–hydroquinone–horseradish peroxidase (HRP) system. Second, a fluidic system was built up with an integrated heating function realized by Peltier elements that allowed a temperature-controlled read-out of the immunosensor in order to study the influence of temperature on the amperometric read-out. The sensor was characterized at the temperature optimum of HRP at 30 °C and at 12 °C as a reference for lower performance. These results were compared to a measurement without temperature control. At the optimum operation temperature of 30 °C, the highest sensitivity (slope) was obtained compared to lower temperatures and a limit of detection of 5.4 ng/mL of IL-13 was calculated. Taken together, this approach is a first step towards an automated electrochemical immunosensor platform and shows the potential of a temperature-controlled read-out. View Full-Text
Keywords: cytokines; electrochemical immunosensor; fluidic system; temperature control cytokines; electrochemical immunosensor; fluidic system; temperature control

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Metzner, J.; Luckert, K.; Lemuth, K.; Hämmerle, M.; Moos, R. Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection. Sensors 2018, 18, 1309.

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