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Sensors 2018, 18(5), 1309; https://doi.org/10.3390/s18051309

Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection

1
Robert Bosch GmbH, Corporate Research, Robert-Bosch-Campus 1, D-71272 Renningen, Germany
2
Department of Functional Materials, University of Bayreuth, Universitätsstraße 30, D-95440 Bayreuth, Germany
*
Author to whom correspondence should be addressed.
Received: 12 March 2018 / Revised: 12 April 2018 / Accepted: 16 April 2018 / Published: 24 April 2018
(This article belongs to the Section Biosensors)
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Abstract

The cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fluidic system with temperature control for read-out. Therefore, two sets of results are presented. First, the sensor was set up in sandwich format on single-walled carbon nanotube electrodes and was read out by applying the hydrogen peroxide–hydroquinone–horseradish peroxidase (HRP) system. Second, a fluidic system was built up with an integrated heating function realized by Peltier elements that allowed a temperature-controlled read-out of the immunosensor in order to study the influence of temperature on the amperometric read-out. The sensor was characterized at the temperature optimum of HRP at 30 °C and at 12 °C as a reference for lower performance. These results were compared to a measurement without temperature control. At the optimum operation temperature of 30 °C, the highest sensitivity (slope) was obtained compared to lower temperatures and a limit of detection of 5.4 ng/mL of IL-13 was calculated. Taken together, this approach is a first step towards an automated electrochemical immunosensor platform and shows the potential of a temperature-controlled read-out. View Full-Text
Keywords: cytokines; electrochemical immunosensor; fluidic system; temperature control cytokines; electrochemical immunosensor; fluidic system; temperature control
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Metzner, J.; Luckert, K.; Lemuth, K.; Hämmerle, M.; Moos, R. Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection. Sensors 2018, 18, 1309.

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