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Sensors 2018, 18(4), 1161;

Label-Free QCM Immunosensor for the Detection of Ochratoxin A

TÜBİTAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey
Department of Medical Genetics and Molecular Biology, Kocaeli University, Umuttepe, Kocaeli 41100, Turkey
Department of Physics, Gebze Technical University, Gebze, Kocaeli 41400, Turkey
These authors contributed equally to this work.
The two authors are out of work now.
Authors to whom correspondence should be addressed.
Received: 3 February 2018 / Revised: 5 March 2018 / Accepted: 8 March 2018 / Published: 11 April 2018
(This article belongs to the Special Issue I3S 2017 Selected Papers)
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Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. View Full-Text
Keywords: OTA; Ochratoxin A; QCM; quartz crystal microbalance; immunobiosensor; regeneration; immobilization OTA; Ochratoxin A; QCM; quartz crystal microbalance; immunobiosensor; regeneration; immobilization

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Pirinçci, Ş.Ş.; Ertekin, Ö.; Laguna, D.E.; Özen, F.Ş.; Öztürk, Z.Z.; Öztürk, S. Label-Free QCM Immunosensor for the Detection of Ochratoxin A. Sensors 2018, 18, 1161.

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