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Sensors 2018, 18(12), 4262; https://doi.org/10.3390/s18124262

Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles

Department of Chemistry, University of Puerto Rico-Mayagüez Campus, Mayagüez 00680, Puerto Rico
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Received: 2 November 2018 / Revised: 19 November 2018 / Accepted: 30 November 2018 / Published: 4 December 2018
(This article belongs to the Section Biosensors)
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Abstract

The recombinant polyhistidine-tagged hemoglobin I ((His)6-rHbI) from the bivalve Lucina pectinata is an ideal biocomponent for a hydrogen sulfide (H2S) biosensor due to its high affinity for H2S. In this work, we immobilized (His)6-rHbI over a surface modified with gold nanoparticles functionalized with 3-mercaptopropionic acid complexed with nickel ion. The attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis of the modified-gold electrode displays amide I and amide II bands characteristic of a primarily α-helix structure verifying the presence of (His)6-rHbI on the electrode surface. Also, X-ray photoelectron spectroscopy (XPS) results show a new peak after protein interaction corresponding to nitrogen and a calculated overlayer thickness of 5.3 nm. The functionality of the immobilized hemoprotein was established by direct current potential amperometry, using H2S as the analyte, validating its activity after immobilization. The current response to H2S concentrations was monitored over time giving a linear relationship from 30 to 700 nM with a corresponding sensitivity of 3.22 × 10−3 nA/nM. These results confirm that the analyzed gold nanostructured platform provides an efficient and strong link for polyhistidine-tag protein immobilization over gold and glassy carbon surfaces for a future biosensors development. View Full-Text
Keywords: protein immobilization; hydrogen sulfide; X-ray photoelectron spectroscopy; Lucina pectinata; His-tag protein protein immobilization; hydrogen sulfide; X-ray photoelectron spectroscopy; Lucina pectinata; His-tag protein
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Torres-González, L.; Díaz-Ayala, R.; Vega-Olivencia, C.A.; López-Garriga, J. Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles. Sensors 2018, 18, 4262.

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