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Open AccessArticle

Label-Free and Redox Markers-Based Electrochemical Aptasensors for Aflatoxin M1 Detection

1
Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia
2
Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Department of Chemical Sciences, National Technical University of Athens, 9 Iroon Polytechniou St., 157 80 Athens, Greece
3
Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, 157 71 Athens, Greece
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(12), 4218; https://doi.org/10.3390/s18124218
Received: 10 November 2018 / Revised: 25 November 2018 / Accepted: 27 November 2018 / Published: 1 December 2018
(This article belongs to the Special Issue Membrane-Based Biosensing)
We performed a comparative analysis of the sensitivity of aptamer-based biosensors for detection mycotoxin aflatoxin M1 (AFM1) depending on the method of immobilization of DNA aptamers and method of the detection. Label-free electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) for ferrocene labeled neutravidin layers were used for this purpose. Amino-modified DNA aptamers have been immobilized at the surface of polyamidoamine dendrimers (PAMAM) of fourth generation (G4) or biotin-modified aptamers were immobilized at the neutravidin layer chemisorbed at gold surface. In the first case the limit of detection (LOD) has been determined as 8.47 ng/L. In the second approach the LOD was similar 8.62 ng/L, which is below of allowable limits of AFM1 in milk and milk products. The aptasensors were validated in a spiked milk samples with good recovery better than 78%. Comparative analysis of the sensitivity of immuno- and aptasensors was also performed and showed comparable sensitivity. View Full-Text
Keywords: biosensors; aflatoxin M1; aptasensors; electrochemical impedance spectroscopy; differential pulse voltammetry biosensors; aflatoxin M1; aptasensors; electrochemical impedance spectroscopy; differential pulse voltammetry
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MDPI and ACS Style

Karapetis, S.; Nikolelis, D.; Hianik, T. Label-Free and Redox Markers-Based Electrochemical Aptasensors for Aflatoxin M1 Detection. Sensors 2018, 18, 4218.

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