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A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection

State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
Authors to whom correspondence should be addressed.
Sensors 2018, 18(10), 3281;
Received: 30 August 2018 / Revised: 19 September 2018 / Accepted: 25 September 2018 / Published: 29 September 2018
(This article belongs to the Special Issue Recent Advances in Nucleic Acid Sensors)
PDF [1779 KB, uploaded 29 September 2018]


In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2 = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106 to 2.988 × 106 M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive. View Full-Text
Keywords: ATP detection; label-free fluorescence; enzyme-free; graphene oxide; logic gate ATP detection; label-free fluorescence; enzyme-free; graphene oxide; logic gate

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhang, J.; Yang, C.; Niu, C.; Liu, C.; Cai, X.; Du, J.; Chen, Y. A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection. Sensors 2018, 18, 3281.

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