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Sensors 2018, 18(1), 105; https://doi.org/10.3390/s18010105

Detection of Ammonia-Oxidizing Bacteria (AOB) Using a Porous Silicon Optical Biosensor Based on a Multilayered Double Bragg Mirror Structure

1
School of Physical Science and Technology, Xinjiang University, Urumqi 830046, China
2
College of Resource and Environment Science, Xinjiang University, Urumqi 830046, China
3
College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China
*
Author to whom correspondence should be addressed.
Received: 22 November 2017 / Revised: 23 December 2017 / Accepted: 28 December 2017 / Published: 1 January 2018
(This article belongs to the Special Issue Recent Advances in Nucleic Acid Sensors)
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Abstract

We successfully demonstrate a porous silicon (PS) double Bragg mirror by electrochemical etching at room temperature as a deoxyribonucleic acid (DNA) label-free biosensor for detecting ammonia-oxidizing bacteria (AOB). Compared to various other one-dimension photonic crystal configurations of PS, the double Bragg mirror structure is quite easy to prepare and exhibits interesting optical properties. The width of high reflectivity stop band of the PS double Bragg mirror is about 761 nm with a sharp and deep resonance peak at 1328 nm in the reflectance spectrum, which gives a high sensitivity and distinguishability for sensing performance. The detection sensitivity of such a double Bragg mirror structure is illustrated through the investigation of AOB DNA hybridization in the PS pores. The redshifts of the reflectance spectra show a good linear relationship with both complete complementary and partial complementary DNA. The lowest detection limit for complete complementary DNA is 27.1 nM and the detection limit of the biosensor for partial complementary DNA is 35.0 nM, which provides the feasibility and effectiveness for the detection of AOB in a real environment. The PS double Bragg mirror structure is attractive for widespread biosensing applications and provides great potential for the development of optical applications. View Full-Text
Keywords: label-free DNA biosensor; porous silicon; photonic crystal; double Bragg mirror structure label-free DNA biosensor; porous silicon; photonic crystal; double Bragg mirror structure
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Zhang, H.; Lv, J.; Jia, Z. Detection of Ammonia-Oxidizing Bacteria (AOB) Using a Porous Silicon Optical Biosensor Based on a Multilayered Double Bragg Mirror Structure. Sensors 2018, 18, 105.

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