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Sensors 2017, 17(7), 1608; https://doi.org/10.3390/s17071608

Rapid ELISA Using a Film-Stack Reaction Field with Micropillar Arrays

1
Graduate School of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan
2
Department of Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
3
Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan
*
Author to whom correspondence should be addressed.
Received: 20 May 2017 / Revised: 26 June 2017 / Accepted: 9 July 2017 / Published: 11 July 2017
(This article belongs to the Section Biosensors)
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Abstract

A film-stack reaction field with a micropillar array using a motor stirrer was developed for the high sensitivity and rapid enzyme-linked immunosorbent assay (ELISA) reaction. The effects of the incubation time of a protein (30 s, 5 min, and 10 min) on the fluorescence intensity in ELISAs were investigated using a reaction field with different micropillar array dimensions (5-µm, 10-µm and 50-µm gaps between the micropillars). The difference in fluorescence intensity between the well with the reaction field of 50-µm gap for the incubation time of 30 s and the well without the reaction field with for incubation time of 10 min was 6%. The trend of the fluorescence intensity in the gap between the micro pillars in the film-stack reaction field was different between the short incubation time and the long incubation time. The theoretical analysis of the physical parameters related with the biomolecule transport indicated that the reaction efficiency defined in this study was the dominant factor determining the fluorescence intensity for the short incubation time, whereas the volumetric rate of the circulating flow through the space between films and the specific surface area were the dominant factors for the long incubation time. View Full-Text
Keywords: ELISA; microbioanalysis device; film-stack reaction field; circulating flow; theoretical equation ELISA; microbioanalysis device; film-stack reaction field; circulating flow; theoretical equation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Suzuki, Y.; Morioka, K.; Ohata, S.; Shimizu, T.; Nakajima, H.; Uchiyama, K.; Yang, M. Rapid ELISA Using a Film-Stack Reaction Field with Micropillar Arrays. Sensors 2017, 17, 1608.

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