Digital Microfluidics for Nucleic Acid Amplification
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i3N|CENIMAT, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal
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UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal
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Authors to whom correspondence should be addressed.
Sensors 2017, 17(7), 1495; https://doi.org/10.3390/s17071495
Received: 27 May 2017
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Revised: 17 June 2017
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Accepted: 22 June 2017
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Published: 25 June 2017
(This article belongs to the Special Issue Microfluidic Sensors and Control Devices)
Digital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical reactions at a much smaller scale. DMF devices open entirely new and promising pathways for multiplex analysis and reaction occurring in a miniaturized format, thus allowing for healthcare decentralization from major laboratories to point-of-care with accurate, robust and inexpensive molecular diagnostics. Here, we shall focus on DMF platforms specifically designed for nucleic acid amplification, which is key for molecular diagnostics of several diseases and conditions, from pathogen identification to cancer mutations detection. Particular attention will be given to the device architecture, materials and nucleic acid amplification applications in validated settings.
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MDPI and ACS Style
Coelho, B.; Veigas, B.; Fortunato, E.; Martins, R.; Águas, H.; Igreja, R.; Baptista, P.V. Digital Microfluidics for Nucleic Acid Amplification. Sensors 2017, 17, 1495. https://doi.org/10.3390/s17071495
AMA Style
Coelho B, Veigas B, Fortunato E, Martins R, Águas H, Igreja R, Baptista PV. Digital Microfluidics for Nucleic Acid Amplification. Sensors. 2017; 17(7):1495. https://doi.org/10.3390/s17071495
Chicago/Turabian StyleCoelho, Beatriz, Bruno Veigas, Elvira Fortunato, Rodrigo Martins, Hugo Águas, Rui Igreja, and Pedro V. Baptista. 2017. "Digital Microfluidics for Nucleic Acid Amplification" Sensors 17, no. 7: 1495. https://doi.org/10.3390/s17071495
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