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Sensors 2017, 17(4), 885;

Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution

The Institute of Scientific and Industrial Research, Osaka University, 8‐1 Mihogaoka, Ibaraki 567-0047, Japan
Author to whom correspondence should be addressed.
Academic Editor: W. Rudolf Seitz
Received: 22 January 2017 / Revised: 11 April 2017 / Accepted: 14 April 2017 / Published: 18 April 2017
(This article belongs to the Special Issue Single-Molecule Sensing)
Full-Text   |   PDF [4290 KB, uploaded 18 April 2017]   |  


Label-free detection of single-nucleotides was performed by fast tunneling current measurements in a polar solvent at 1 MHz sampling rate using SiO2-protected Au nanoprobes. Short current spikes were observed, suggestive of trapping/detrapping of individual nucleotides between the nanoelectrodes. The fall and rise features of the electrical signatures indicated signal retardation by capacitance effects with a time constant of about 10 microseconds. The high temporal resolution revealed current fluctuations, reflecting the molecular conformation degrees of freedom in the electrode gap. The method presented in this work may enable direct characterizations of dynamic changes in single-molecule conformations in an electrode gap in liquid. View Full-Text
Keywords: tunneling current; single molecule; sequencing tunneling current; single molecule; sequencing

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Morikawa, T.; Yokota, K.; Tanimoto, S.; Tsutsui, M.; Taniguchi, M. Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution. Sensors 2017, 17, 885.

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