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Open AccessArticle

A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops

Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Spain
Departamento de Química, Centro de Ciências da Natureza, Universidade Federal do Piauí, Teresina 64049-550PI, Brazil
Instituto de Ciencias Biológicas, Universidade de Pernambuco, Recife 50670-901PE, Brazil
Author to whom correspondence should be addressed.
Academic Editor: Nicole Jaffrezic-Renault
Sensors 2017, 17(4), 881;
Received: 14 March 2017 / Revised: 10 April 2017 / Accepted: 12 April 2017 / Published: 18 April 2017
(This article belongs to the Special Issue Genosensing)
The design of screening methods for the detection of genetically modified organisms (GMOs) in food would improve the efficiency in their control. We report here a PCR amplification method combined with a sequence-specific electrochemical genosensor for the quantification of a DNA sequence characteristic of the 35S promoter derived from the cauliflower mosaic virus (CaMV). Specifically, we employ a genosensor constructed by chemisorption of a thiolated capture probe and p-aminothiophenol gold surfaces to entrap on the sensing layer the unpurified PCR amplicons, together with a signaling probe labeled with fluorescein. The proposed test allows for the determination of a transgene copy number in both hemizygous (maize MON810 trait) and homozygous (soybean GTS40-3-2) transformed plants, and exhibits a limit of quantification of at least 0.25% for both kinds of GMO lines. View Full-Text
Keywords: electrochemical genosensor; genetically modified crops; PCR; screening GMOs electrochemical genosensor; genetically modified crops; PCR; screening GMOs
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Moura-Melo, S.; Miranda-Castro, R.; De-los-Santos-Álvarez, N.; Miranda-Ordieres, A.J.; Dos Santos Junior, J.R.; Da Silva Fonseca, R.A.; Lobo-Castañón, M.J. A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors 2017, 17, 881.

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