A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops
Abstract
:1. Introduction
2. Materials and Methods
2.1. Instrumentation
2.2. Reagents
2.3. Sample Preparation and DNA Extraction
2.4. Real-Time Polymerase Chain Reaction (qPCR)
2.5. End-Point Polymerase Chain Reaction (PCR)
2.6. Agarose Gel Electrophoresis
2.7. Electrochemical Genosensor
3. Results and Discussion
3.1. PCR Amplification: Design and Evaluation
3.2. Combining PCR and Electrochemical Genosensor
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sequence Name | Length | Oligonucleotide Sequences 5′ → 3′ |
---|---|---|
Capture probe (CP) | 27 nt | AGA GGA AGG GTC TTG CGA AGG ATA GTG-(CH2)6SH |
Signaling probe (SP) | 53 nt | 1FITC-CTA GAG TCA GCT TGT CAG CGT GTC CTC TCC AAA TGA AAT GAA CTT CCT TAT AT |
P35S-Forward primer (P35S-FP) | 27 nt | GTA AGG GAT GAC GCA CAA TCC CAC TAT |
P35S-Reverse primer (P35S-RP) | 27 nt | TCT GCT AGA GTC AGC TTG TCA GCG TGT |
Plant-Forward primer (Plant-FP) | 23 nt | CTT GAT TTT ACC AAA GAT GAT GA |
Plant-Reverse primer (Plant-RP) | 20 nt | TTC TTC GCA TGT ACC CGC AG |
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Moura-Melo, S.; Miranda-Castro, R.; De-los-Santos-Álvarez, N.; Miranda-Ordieres, A.J.; Dos Santos Junior, J.R.; Da Silva Fonseca, R.A.; Lobo-Castañón, M.J. A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors 2017, 17, 881. https://doi.org/10.3390/s17040881
Moura-Melo S, Miranda-Castro R, De-los-Santos-Álvarez N, Miranda-Ordieres AJ, Dos Santos Junior JR, Da Silva Fonseca RA, Lobo-Castañón MJ. A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors. 2017; 17(4):881. https://doi.org/10.3390/s17040881
Chicago/Turabian StyleMoura-Melo, Suely, Rebeca Miranda-Castro, Noemí De-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, José Ribeiro Dos Santos Junior, Rosana A. Da Silva Fonseca, and María Jesús Lobo-Castañón. 2017. "A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops" Sensors 17, no. 4: 881. https://doi.org/10.3390/s17040881
APA StyleMoura-Melo, S., Miranda-Castro, R., De-los-Santos-Álvarez, N., Miranda-Ordieres, A. J., Dos Santos Junior, J. R., Da Silva Fonseca, R. A., & Lobo-Castañón, M. J. (2017). A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors, 17(4), 881. https://doi.org/10.3390/s17040881