Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping
AbstractColorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%–60% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor (EGFR) agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the main KRAS mutations on the portable real-time PCR Q3 platform. This platform is based on hybrid silicon-plastic technology implemented in a miniaturized chip able to achieve a sample-in answer-out rapid analysis, allowing a new approach to genetic counseling and testing. View Full-Text
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Guarnaccia, M.; Iemmolo, R.; Petralia, S.; Conoci, S.; Cavallaro, S. Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping. Sensors 2017, 17, 831.
Guarnaccia M, Iemmolo R, Petralia S, Conoci S, Cavallaro S. Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping. Sensors. 2017; 17(4):831.Chicago/Turabian Style
Guarnaccia, Maria; Iemmolo, Rosario; Petralia, Salvatore; Conoci, Sabrina; Cavallaro, Sebastiano. 2017. "Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping." Sensors 17, no. 4: 831.