Next Article in Journal
A Hierarchical Building Segmentation in Digital Surface Models for 3D Reconstruction
Next Article in Special Issue
Theoretical and Experimental Comparison of Different Formats of Immunochromatographic Serodiagnostics
Previous Article in Journal
Intelligent RF-Based Gesture Input Devices Implemented Using e-Textiles
Open AccessArticle

Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor

1
The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China
2
The 2011 Project Collaborative Innovation Center for Biological Therapy, Nankai University, Tianjin 300071, China
*
Author to whom correspondence should be addressed.
Academic Editor: Robert Rubin
Sensors 2017, 17(2), 221; https://doi.org/10.3390/s17020221
Received: 19 November 2016 / Revised: 14 January 2017 / Accepted: 18 January 2017 / Published: 24 January 2017
(This article belongs to the Special Issue Biosensors for Antibody Detection)
Protein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO). With additional antibody-antigen binding, the RGOM-based biosensor achieved better real-time and label-free detection. The RGOM-based biosensor presented a more satisfactory response to goat anti-rabbit IgG than the RGO-based biosensor. This method is promising for immobilizing biomolecules on graphene surfaces and for the fabrication of biosensors with enhanced sensitivity. View Full-Text
Keywords: reduced graphene oxide; microshells; optical sensor; goat anti-rabbit IgG; real-time; label-free reduced graphene oxide; microshells; optical sensor; goat anti-rabbit IgG; real-time; label-free
Show Figures

Figure 1

MDPI and ACS Style

Jiang, W.-S.; Xin, W.; Chen, S.-N.; Li, C.-B.; Gao, X.-G.; Pan, L.-T.; Liu, Z.-B.; Tian, J.-G. Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor. Sensors 2017, 17, 221.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop