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Sensors 2017, 17(11), 2620;

Analytical Parameters of an Amperometric Glucose Biosensor for Fast Analysis in Food Samples

Electrochemical Methods Laboratory—Analytical and Applied Chemistry Department, ETS Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta, 390, 08017 Barcelona, Spain
Author to whom correspondence should be addressed.
Received: 28 September 2017 / Revised: 2 November 2017 / Accepted: 6 November 2017 / Published: 14 November 2017
(This article belongs to the Special Issue I3S 2017 Selected Papers)
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Amperometric biosensors based on the use of glucose oxidase (GOx) are able to combine the robustness of electrochemical techniques with the specificity of biological recognition processes. However, very little information can be found in literature about the fundamental analytical parameters of these sensors. In this work, the analytical behavior of an amperometric biosensor based on the immobilization of GOx using a hydrogel (Chitosan) onto highly ordered titanium dioxide nanotube arrays (TiO2NTAs) has been evaluated. The GOx–Chitosan/TiO2NTAs biosensor showed a sensitivity of 5.46 μA·mM−1 with a linear range from 0.3 to 1.5 mM; its fundamental analytical parameters were studied using a commercial soft drink. The obtained results proved sufficient repeatability (RSD = 1.9%), reproducibility (RSD = 2.5%), accuracy (95–105% recovery), and robustness (RSD = 3.3%). Furthermore, no significant interferences from fructose, ascorbic acid and citric acid were obtained. In addition, the storage stability was further examined, after 30 days, the GOx–Chitosan/TiO2NTAs biosensor retained 85% of its initial current response. Finally, the glucose content of different food samples was measured using the biosensor and compared with the respective HPLC value. In the worst scenario, a deviation smaller than 10% was obtained among the 20 samples evaluated. View Full-Text
Keywords: biosensor; chitosan; glucose oxidase; glucose quantification biosensor; chitosan; glucose oxidase; glucose quantification

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Artigues, M.; Abellà, J.; Colominas, S. Analytical Parameters of an Amperometric Glucose Biosensor for Fast Analysis in Food Samples. Sensors 2017, 17, 2620.

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