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Sensors 2017, 17(10), 2246; https://doi.org/10.3390/s17102246

A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer

1
Department of Food and Pharmaceutical Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China
2
Department of Chemistry, Wuhan University, Wuhan 430072, China
Yingying Hu and Jingjing Liu contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 August 2017 / Revised: 11 September 2017 / Accepted: 18 September 2017 / Published: 29 September 2017
(This article belongs to the Special Issue Fluorescent Probes and Sensors)
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Abstract

A light-up fluorescent probe for the detection of adenosine was constructed with an AIE (aggregation-induced emission) molecule and a DNA aptamer. The AIE molecule was used as a signal generator, and the DNA aptamer was used as a recognition element for adenosine. The emission of the AIE molecule was due to its intramolecular rotation restriction induced by the aptamer upon binding of adenosine. The optimal component ratio of the probe was AIE molecule / DNA aptamer = 100 (μM/μM). The calibration curve of adenosine detection showed a linear range of 10 pM to 0.5 μM with an R2 of 0.996, and the detection limit of the probe was 10 pM. The probe exhibited a good selectivity to adenosine against its analogs (uridine, guanosine, and cytidine). The probe was used to detect adenosine in urine samples, a recovery from 86.8% to 90.0% for the spiked concentrations of adenosine (0.01, 0.05, 0.1 μM). The relative standard deviation from 1.2% to 2.0% was obtained. The intra-day and inter-day tests also showed good precisions, with measurement RSD values of 2.3% and 2.1%, respectively. View Full-Text
Keywords: aggregation-induced emission; aptamer; adenosine; 1,2-bis[4-(triethylammoniomethyl)phenyl]-1,2-diphenylethenedibromide (TPE-2N+) aggregation-induced emission; aptamer; adenosine; 1,2-bis[4-(triethylammoniomethyl)phenyl]-1,2-diphenylethenedibromide (TPE-2N+)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hu, Y.; Liu, J.; You, X.; Wang, C.; Li, Z.; Xie, W. A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer. Sensors 2017, 17, 2246.

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