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Open AccessArticle

ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
Environment Monitoring Department, Changsha Environmental Protection College, Changsha 410004, China
Author to whom correspondence should be addressed.
Academic Editor: Jong Seung Kim
Sensors 2016, 16(10), 1684;
Received: 24 August 2016 / Revised: 20 September 2016 / Accepted: 22 September 2016 / Published: 12 October 2016
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensor)
Photoactivatable fluorophores have become an important technique for the high spatiotemporal resolution of biological imaging. Here, we developed a novel photoactivatable probe (PHBT), which is based on 2-(2-hydroxyphenyl)benzothiazole (HBT), a small organic fluorophore known for its classic luminescence mechanism through excited-state intramolecular proton transfer (ESIPT) with the keto form and the enol form. After photocleavage, PHBT released a ratiometric fluorophore HBT, which showed dual emission bands with more than 73-fold fluorescence enhancement at 512 nm in buffer and more than 69-fold enhancement at 452 nm in bovine serum. The probe displayed a high ratiometric imaging resolution and is believed to have a wide application in biological imaging. View Full-Text
Keywords: ESIPT; photoactivatable; ratiometric ESIPT; photoactivatable; ratiometric
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MDPI and ACS Style

Zhou, X.; Jiang, Y.; Zhao, X.; Guo, D. ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging. Sensors 2016, 16, 1684.

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