Next Article in Journal
Operational Safety Assessment of Turbo Generators with Wavelet Rényi Entropy from Sensor-Dependent Vibration Signals
Next Article in Special Issue
Development of a HIV-1 Virus Detection System Based on Nanotechnology
Previous Article in Journal
Monitoring the Corrosion Process of Reinforced Concrete Using BOTDA and FBG Sensors
Previous Article in Special Issue
Rapid Detection of Ebola Virus with a Reagent-Free, Point-of-Care Biosensor
Open AccessArticle

Comparison of Whole-Cell SELEX Methods for the Identification of Staphylococcus Aureus-Specific DNA Aptamers

National Academy of Agricultural Science, 310 Nongsaengmyeng-ro, Wansan-gu, Jeonju 560500, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Stephane Evoy
Sensors 2015, 15(4), 8884-8897; https://doi.org/10.3390/s150408884
Received: 6 February 2015 / Revised: 28 March 2015 / Accepted: 9 April 2015 / Published: 15 April 2015
(This article belongs to the Special Issue Biosensors for Pathogen Detection)
Whole-cell Systemic Evolution of Ligands by Exponential enrichment (SELEX) is the process by which aptamers specific to target cells are developed. Aptamers selected by whole-cell SELEX have high affinity and specificity for bacterial surface molecules and live bacterial targets. To identify DNA aptamers specific to Staphylococcus aureus, we applied our rapid whole-cell SELEX method to a single-stranded ssDNA library. To improve the specificity and selectivity of the aptamers, we designed, selected, and developed two categories of aptamers that were selected by two kinds of whole-cell SELEX, by mixing and combining FACS analysis and a counter-SELEX process. Using this approach, we have developed a biosensor system that employs a high affinity aptamer for detection of target bacteria. FAM-labeled aptamer sequences with high binding to S. aureus, as determined by fluorescence spectroscopic analysis, were identified, and aptamer A14, selected by the basic whole-cell SELEX using a once-off FACS analysis, and which had a high binding affinity and specificity, was chosen. The binding assay was evaluated using FACS analysis. Our study demonstrated the development of a set of whole-cell SELEX derived aptamers specific to S. aureus; this approach can be used in the identification of other bacteria. View Full-Text
Keywords: aptamer; biosensor; pathogen detection; whole-cell SELEX aptamer; biosensor; pathogen detection; whole-cell SELEX
Show Figures

Figure 1

MDPI and ACS Style

Moon, J.; Kim, G.; Park, S.B.; Lim, J.; Mo, C. Comparison of Whole-Cell SELEX Methods for the Identification of Staphylococcus Aureus-Specific DNA Aptamers. Sensors 2015, 15, 8884-8897.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop