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Sensors 2015, 15(2), 3854-3871;

Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor

Sensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South Africa
Author to whom correspondence should be addressed.
Received: 22 October 2014 / Revised: 17 November 2014 / Accepted: 7 December 2014 / Published: 6 February 2015
(This article belongs to the Special Issue Immunosensors 2014)
Full-Text   |   PDF [2169 KB, uploaded 6 February 2015]


In this paper, we report a new concept to construct a label-free electrochemical inhibition-based immunosensor for the detection of the mycotoxin deoxynivalenol (DON) in cereal samples. The electrochemical impedance spectroscopy of tris(bipyridine) ruthenium (II) chloride was used as a marker enhanced with gold nanoparticles-dotted 4-nitrophenylazo functionalized graphene (AuNp/G/PhNO2) nanocatalyst mediated in Nafion on a glassy carbon electrode. Under the optimized conditions, the formation of immunocomplexes inhibited electron flow and increased the charge transfer resistance of the sensing interface linearly. The change in impedance was proportional to DON concentrations in the range of 6–30 ng/mL with a sensitivity and detection limit of 32.14 ΩL/ng and 0.3 µg/mL, respectively, which compares favorably with the ELISA result. The proposed sensor had a stability of 80.3%, good precision and selectivity in DON standard solution containing different interfering agents, indicating promising application prospect for this strategy in designing impedimetric, electrochemiluminescent, voltammetric or amperometric sensors. View Full-Text
Keywords: deoxynivalenol; mycotoxin; graphene; immunosensor; graphene oxide; Nafion deoxynivalenol; mycotoxin; graphene; immunosensor; graphene oxide; Nafion
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sunday, C.E.; Masikini, M.; Wilson, L.; Rassie, C.; Waryo, T.; Baker, P.G.L.; Iwuoha, E.I. Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor. Sensors 2015, 15, 3854-3871.

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