Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries
Share Yuan Goh 1, Wen-Si Tan 1, Saad Ahmed Khan 2, Hooi Pin Chew 2, Noor Hayaty Abu Kasim 2, Wai-Fong Yin 1 and Kok-Gan Chan 1,*
1
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
2
Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
*
Author to whom correspondence should be addressed.
Sensors 2014, 14(5), 8940-8949; https://doi.org/10.3390/s140508940
Received: 18 April 2014 / Revised: 4 May 2014 / Accepted: 13 May 2014 / Published: 21 May 2014
(This article belongs to the Section Biosensors)
Abstract
Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of a QS bacterial isolate C10B from dentine caries. Preliminary screening using Chromobacterium violaceum CV026 biosensor showed that isolate C10B was able to produce N-acylhomoserine lactones (AHLs). This bacterium was further identified as a member of Burkholderia, an opportunistic pathogen. The isolated Burkholderia sp. was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL) and N-dodecanoyl-L-homoserine lactone (C12-HSL). View Full-TextKeywords:
Chromobacterium violaceum CV026; N-hexanoyl-L-homoserine lactone (C6-HSL); N-octanoyl-L-homoserine lactone (C8-HSL); N-decanoyl-L-homoserine lactone (C10-HSL); N-dodecanoyl-L-homoserine lactone (C12-HSL); oral cavity; dentine caries; quorum sensing (QS)
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MDPI and ACS Style
Goh, S.Y.; Tan, W.-S.; Khan, S.A.; Chew, H.P.; Kasim, N.H.A.; Yin, W.-F.; Chan, K.-G. Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries. Sensors 2014, 14, 8940-8949.