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Sensors 2013, 13(9), 12516-12526;

Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma

Department of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA
School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY 14642, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 16 July 2013 / Revised: 6 September 2013 / Accepted: 11 September 2013 / Published: 17 September 2013
(This article belongs to the Special Issue Biomimetic Receptors and Sensors)
Full-Text   |   PDF [543 KB, uploaded 21 June 2014]


The isolation of hematopoietic stem and progenitor cells (HSPCs) is critical for transplantation therapy and HSPC research, however current isolation techniques can be prohibitively expensive, time-consuming, and produce variable results. Selectin-coated microtubes have shown promise in rapidly isolating HSPCs from human bone marrow, but further purification of HSPCs remains a challenge. Herein, a biomimetic device for HSPC isolation is presented to mimic the acidic vascular microenvironment during trauma, which can enhance the binding frequency between L-selectin and its counter-receptor PSGL-1 and HSPCs. Under acidic pH conditions, L-selectin coated microtubes enhanced CD34+ HSPC adhesion, as evidenced by decreased cell rolling velocity and increased rolling flux. Dynamic light scattering was utilized as a novel sensor to confirm an L-selectin conformational change under acidic conditions, as previously predicted by molecular dynamics. These results suggest that mimicking the acidic conditions of trauma can induce a conformational extension of L-selectin, which can be utilized for flow-based, clinical isolation of HSPCs. View Full-Text
Keywords: CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH CD34+ hematopoietic stem cells; L-selectin; biomimetic; acidic pH
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Cao, T.M.; Mitchell, M.J.; Liesveld, J.; King, M.R. Stem Cell Enrichment with Selectin Receptors: Mimicking the pH Environment of Trauma. Sensors 2013, 13, 12516-12526.

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