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Article

Polychaete Diversity and Functional Trait Composition in Subtropical Mangrove Ecosystems

by
Mohadeseh Miri
1,
Jafar Seyfabadi
1,*,
Mehdi Ghodrati Shojaei
1,
Hassan Rahimian
2 and
Mohammad Valipour
3,*
1
Department of Marine Biology, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor 4641776489, Iran
2
School of Biology, College of Science, University of Tehran, Tehran 14155-6655, Iran
3
Department of Engineering and Engineering Technology, Metropolitan State University of Denver, Denver, CO 80217, USA
*
Authors to whom correspondence should be addressed.
Diversity 2023, 15(9), 998; https://doi.org/10.3390/d15090998
Submission received: 4 August 2023 / Revised: 1 September 2023 / Accepted: 5 September 2023 / Published: 7 September 2023
(This article belongs to the Special Issue Investigation of the Biology, Ecology, Distribution of Marine Macrozoobenthic Communities)
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Abstract

:
Polychaetes play a vital role in the structure and functioning of benthic communities in mangrove ecosystems. Nevertheless, our understanding of the diversity and functional structure of polychaete assemblages across different habitats in the mangrove ecosystems along the coast of the Persian Gulf and Gulf of Oman is limited. In this study, we investigated the species and trait composition of polychaetes and environmental variables, in vegetated and mudflat habitats of three subtropical mangroves. The results showed that Neanthes glandicincta was widely distributed across all regions and habitats. The three-factor ANOVA showed that the abundance and taxonomic diversity of polychaetes differed significantly between two habitats and three mangrove ecosystems. The abundance of polychaetes was observed to be higher in mud habitats than in vegetated habitats. There was a significant difference in species and trait composition between different regions and habitats. Vegetated habitats had higher proportions of crawler predatory species that are longer lived (3–5 years), with larger body size (80–100 mm), and are upward conveyors, whereas mudflat habitats had higher proportions of mobile (burrower) omnivore species that are moderately lived (1–3 years), with larger body size (>100 mm), and are biodiffusers. The three-factor ANOVA showed a significant difference in the community weighted mean (CWM) index between two habitats and three mangrove ecosystems. Thus, the species and trait composition of polychaetes depend on the structural complexity of their respective habitats. The DistLM analysis showed that total organic carbon content of the sediment was the main predictor variable influencing species composition, while silt/clay content and salinity were the main predictor variables influencing the traits’ composition. The results showed how the composition of traits and the structure of polychaete communities change in mangrove ecosystems, which can be used for future studies on conservation strategies for mangrove ecosystems throughout the world.

Graphical Abstract

1. Introduction

Mangrove ecosystems grow along the shallow coastlines of tropical and subtropical regions [1]. They are one of the most biologically diverse ecosystems, rich in organic matter and nutrients that support a large biomass of plants and animals [2]. There are a number of natural and anthropogenic disturbances that threaten mangroves, including changes in hydrodynamics, subsidence, pollution from industries, clearing of mangrove trees, and climate change [3]. Globally, mangrove areas decline by 0.22% per year [4,5].
The infauna play essential roles in the secondary production of a sediment, bioturbation, and nutrient cycling [6]. They display various nutritional behaviors in mangrove ecosystems, which can be used as bioindicators of the ecosystem’s structure and environmental conditions and disturbances [7]. Polychaetes constitute a macrobenthic group of great abundance and diversity in marine ecosystems, including mangroves. Additionally, they are eaten by seabirds, fish, and crustaceans with commercial value. As a result of their abundance, diverse feeding habits, occupation of niches, and the relationship to different sediment types, they play an important role in the structure and functioning of benthic communities [8]. Also, polychaetes are active participants in a variety of marine ecological processes, including bioturbation, nutrient cycling, secondary production, and energy flow [9,10].
The change in environmental conditions strongly affects the community structure (species composition and diversity) and consequently the overall functioning of ecosystems [11,12]. Increasing evidence suggests that species diversity and species richness alone are not sufficient to explain or predict ecosystem functions [10,13] and a species’ ability to cope with environmental disturbance is at least partly driven by its traits [9,14,15]. These traits include morphological, behavioral, and physiological characteristics that can explain the interaction between species and their environment, conspecifics, and individuals of other species [16,17]. A biological trait analysis (BTA) has been proposed as a useful approach for describing the ecological functioning of marine ecosystems. BTA was first used in freshwater ecosystems [18,19] and has progressively been applied to marine benthic communities [20,21,22,23,24,25]. As one of the trait-based indices, the community weighted mean value (CWM) is a value in a community that shows the number of species expressing a specific trait in a given community [12] weighted by the relative abundance of the species carrying each value in the community. It is increasingly used to evaluate the response of communities to disturbances that can manage “multiple” different traits [9,22]. The CWM approach can be used to analyze shifts in mean traits within communities as the result of the environmental selection for certain traits [22]. It presents the underlying qualitative features of the traits expressed and their potential implications for ecosystem function.
The Persian Gulf and Gulf of Oman experience extreme environmental pressures, including salinity and temperature fluctuations, which place organisms at the limits of their physiological tolerance to environmental conditions [26]. As a diverse and important coastal habitat, mangroves are threatened by natural and anthropogenic pressures, which makes further studies of their biodiversity and function imperative. The structural complexity of mangroves plays a crucial role in the biological organization of communities by promoting species coexistence by reducing niche overlap, mediating predation by providing refuge for smaller organisms, and altering the physical environment [27]. There have been some studies that have evaluated the influence of mangrove vegetation on the functional diversity of invertebrates and the consequent changes in ecosystem functioning [28,29,30,31,32]. Although some studies have also been conducted on the taxonomic and functional diversity of macrofaunal communities of mangrove forests along the coasts of the Persian Gulf and Gulf of Oman [22,23,24], no study has been conducted on the taxonomic diversity and functional trait composition of polychaete assemblages. The main objectives of this study are to (i) compare the species and functional trait composition between vegetated and mudflat habitats and (ii) investigate the effects of environmental conditions and habitat complexity driven by features associated with mangrove trees on species and trait composition in three subtropical mangrove ecosystems.

2. Materials and Methods

2.1. Study Area

There are 94.03 km2 of mangrove forests in Iran, located in the geographic range between 25°11′ and 27°52′ N [33]. Avicennia marina and Rhizophora mucronata are the two species of mangrove forest that grow in Iran, but the latter is found only in the estuaries of Sirik [34]. The Nayband estuary is in the geographic range of 52°24′58″ to 52°38′58″ E and 27°22′58″ to 27°39′58″ N. There are about 3.9 km2 of mangrove forest in Nayband Bay, which has a subtropical climate. During the winter months, the average temperature is 12–16 °C, while during the summer months, it reaches 42 °C. Through Hale Channel, tidal water exchanges with the open sea without major freshwater discharges into the forest [34]. The Sirik estuary with an area of over 270 km2 is located in-between 57°11′ and 57°20′ E and 26°10′ and 26°26′ N. Air temperature in winter and summer exceeds 10 and 50 °C, respectively. This forest is normally affected by freshwater carried by Gaz River [35]. A large portion of Gwadar Bay lies within Pakistan (about 69%) and about 31% in Iran. The Iranian part is located at 25°1′ and 25°12′ N and 61°34′ and 61°47′ E. The area of mangroves in the Gwadar estuary is 1.593 km2. The average air temperature was 42 °C in summer and 20 °C in winter. The Gwadar forest is normally affected by freshwater carried by Bahu Kalat River [36]. The mangrove forests of these three estuaries are listed in the Ramsar Convention as protected areas and are among the most important mangrove ecosystems in Iran.

2.2. Sampling of Polychaetes

Polychaetes were sampled from three mangrove regions of Nayband (Persian Gulf), Sirik, and Gwadar (Gulf of Oman), during the warmest (July) and coldest (January) seasons in 2019. Randomly, 5 plots were chosen in each region (Figure 1). Each plot consisted of vegetated (with trees and pneumatophores) and mudflat (without trees and pneumatophores) habitats. In each habitat, one random plot was centered at least 8 m from the forest fringe to avoid edge effects that could affect the community composition. Three random sediment samples were collected from each habitat using a metal 25 × 25 cm quadrat, with a depth of 25 cm.
The sediment samples were washed with sea water on a 0.5 mm sieve until clear water emerged. The residue containing polychaetes was preserved using 70% ethanol. In a laboratory, the polychaetes were collected, sorted, and identified to the lowest possible taxonomic group using identification sources [37,38,39,40,41] and a species–sites matrix was created.

2.3. Environmental Data

Temperature (°C) and salinity (PPT) of water were measured in situ using a HACH Multi-parameter device that had been recently calibrated. To measure total organic carbon (TOC), total organic nitrogen (TON), and sediment grain size, three replicates of the sediment were collected from each habitat using volumetric soil cores (internal diameter—5 cm, depth—10 cm). The Micro–Kjeldahl method [42] and the Walkley–Black method [43] were used to measure total organic nitrogen (TON) and total organic carbon (TOC), respectively. The particle size of the sediment was determined using the hydrometer technique [44].

2.4. Trait Data

Seven biological traits, composed of 34 modalities, were selected to investigate polychaete functional roles and patterns in the mangrove forests (Table 1). To quantify the affinity of each species to each modality, fuzzy coding was used [45], in which species are assigned to scores ranging from 0 to 3 for each modality (0 = no affinity, 1 = low affinity, 2 = moderate affinity, and 3 = high affinity). The classification of polychaetes was collected from online databases (e.g., http://www.marlin.ac.uk/biotic/; accessed on 25 March 2023, and http://polytraits.lifewatchgreece.eu/; accessed on 25 March 2023), the peer-reviewed literature, and an identification key [7,37,40,41,46]. A matrix was created listing each species’ affinity to the different trait modalities and showing the abundance of biological traits for each combination of a region, habitat, and season. CWM was calculated, using the ‘trait-by-station’ matrix, to analyze differences in the composition and expression of functional characteristics in the mangrove ecosystems, which may reflect the trait strategies exhibited by a region’s species pool and environmental conditions [47]. Every replicate sample was defined as a community for calculating CWM.

2.5. Data Analysis

For each multivariate analysis, the two matrices (i.e., species composition and CWM values) were square root transformed, and Bray–Curtis similarity was calculated [48].
The abundance data of the polychaetes were used in PRIMER 6.0 to calculate species diversity indices for each habitat in the regions, including the Margalef species richness index (d), the Pielou evenness index (J’), the Shannon–Wiener diversity index (H’), and the Simpson dominance index.
The non-metric multidimensional scaling analysis (nMDS) ordination model was applied to the taxa abundance matrix to analyze species and trait composition. After verifying normality and homoscedasticity, each analysis was performed. A SIMPER analysis was used to assess the dissimilarity between regions, habitats, and seasons regarding their species and trait modalities composition, and to identify which species and traits contributed most to these significant differences.
A distance-based linear model (DistLM) was used to evaluate the relative contribution of abiotic parameters to the variability observed in species and trait composition of each mangrove region. The BEST selection procedure was applied to the DistLM model in order to select the best combination of predictor variables using the AIC (Akaike’s Information Criteria). Subsequently, a distance-based redundancy analysis (dbRDA) was performed on the fitted values obtained from the given model built with DISTLM [49].
To assess differences in abundance, taxonomic diversity, and the CWM index between regions, habitats, and sampling seasons, we used a repeated measure three-factor ANOVA, where ‘region’ and ‘season’ were fixed factors and ‘habitat’ was a random factor. All tests were considered significant if the p-value was less than 0.05.
Multivariate analyses were conducted using PRIMER v6 with the PERMANOVA add-on package. The CWM index was calculated in R v4.1.0 software using the package FD, ade4, and ggplot2 for each sampling habitat and season [50]. Also, the three-factor ANOVA was performed with statistical software R [51].

3. Results

A total of 33 species belonging to 16 families were identified in the three mangrove regions. The most abundant species was Neanthes glandicincta in Gwadar (709.45 ± 105.9 ind.m−2) and Sirik (255.42 ± 35.12 ind.m−2) regions, while the most abundant species was Simplisetia erythraeensis in the Nayband region (75 ± 11.87 ind.m−2). Generally, the most abundant families were Nereididae, Capitellidae, and Paraonidae in the three studied regions (Table S1).
The three-factor ANOVA showed a significant difference in polychaete abundance in Gwadar, Sirik, and Nayband regions (Table 2). The lowest and highest abundances were observed in Nayband mudflats during winter (16 ± 0.64 ind.m−2) and Sirik mudflats during summer (640 ± 45 ind.m−2), respectively (Figure 2a). In the summer, no species were observed in the vegetated and mudflat habitats of Nayband. The abundance was significantly higher in mudflat than in vegetated habitats. The three-factor ANOVA also revealed significant differences in the polychaete abundance between seasons (p < 0.05).
In winter, the Shannon diversity index showed the lowest value in Nayband (0.02 ± 0.008) and the highest value in Gwadar (0.83 ± 0.38) (Figure 2b). Based on the three-factor ANOVA, Shannon’s index differed significantly between the three regions and two habitats (p < 0.05), being higher in mudflats than in vegetated habitats (Table 2 and Figure 2b). The Margalef richness index ranged from 0.1 ± 0.07 to 0.43 ± 0.12 (Figure 2c), and the three-factor ANOVA indicated that the polychaete community structure differed significantly between the regions and habitats (p < 0.05). The three-factor ANOVA also revealed a significant difference in Shannon diversity and Margalef richness between seasons (p < 0.05) (Table 2). The lowest and highest values of the evenness index were 0.19 ± 0.08 and 0.91 ± 0.32, for vegetated habitats of Nayband in winter and vegetated habitats of Sirik in summer, respectively (Figure 2d). The mean Simpson’s dominant index values varied between 0.52 ± 0.23 and 1 in the three studied regions, with the lowest and highest values calculated in the mudflats of Nayband and Gwadar in winter (Figure 2e). Pielou’s evenness and Simpson’s dominant indices differed significantly between regions (p < 0.05), whereas there was no significant difference between the seasons and habitats (p > 0.05).
Average values of CWM were plotted to visualize a functional trait composition for each habitat and season in each mangrove region (Figure 3). Gwadar’s dominant trait modalities were a size > 100, biodiffuser, omnivore, life span of 1–3 years, burrower, and AMBI-IV. The dominant trait modalities were a size > 100 mm, biodiffuser, omnivore, life span of 1–3 and 3–5 years, burrower, and AMBI-I in Sirik. The highest values of CWM were obtained with a size of 10–20 and > 100 mm, biodiffuser, omnivore–predator, life span of 1–3 and 3–5 years, crawler, and AMBI-IV in Nayband (Figure 3). The results of the three-factor ANOVA revealed significant differences in the community weighted mean (CWM) between regions and habitats, while there were no significant differences between seasons (Table 2).
The composition of trait modalities for each trait was almost the same in all habitats, but the percentage contribution varied, which explained the observed differences. Species inhabiting vegetated habitats tended to be dominated by crawling predators and moderately large (80–100 mm), relatively long-lived (3–5 years), and upward conveyors, whereas species inhabiting mudflat habitats tended to be dominated by omnivores, larger bodies (>100 mm), relatively moderate-lived (1–3 years), burrowers, and biodiffusers (Figure 3).
Non-metric multidimensional scaling (nMDS) based on polychaete species and trait composition highlighted a higher variability of assemblages between habitats and regions rather than seasons (Figure 4). Overall, the spatial dissimilarity between two habitats (vegetated and mudflat) and three regions (Gwadar, Sirik, and Nayband) was confirmed with the three-factor ANOVA analysis (p < 0.05, Table 2).
Based on the SIMPER analysis of polychaete abundance, Gwadar and Sirik had a dissimilarity of 83.91%. The species Neanthes glandicincta contributed significantly to Gwadar and Sirik divisions (cont. = 27.61%). The dissimilarity between Gwadar and Nayband was 39.99%, mainly caused by Simplisetia erythraeensis (cont. = 15.16%). The dissimilarity between Sirik and Nayband (average dissimilarity = 100%) was mainly because of the N. gladicincta (cont. = 23.28), S. erythraeensis (cont. = 20.26), and Perinereis horsti (cont. = 19.92). The average dissimilarity between habitats (vegetated and mudflats) and seasons (winter and summer) was 83.83% and 79.38%, respectively. In particular, N. gladicincta contributed mainly to the dissimilarity between habitats (cont. = 33.02%) and seasons (cont. = 31.55%) (Table S2).
According to the SIMPER analysis, the average dissimilarity between Gwadar and Sirik (dissimi. = 30.41%) was mainly due to the upward conveyor (cont. = 4.39%) and deposit feeder (selective or non-selective) (cont. = 4.39%). There was an average dissimilarity of 52.95% between Gwadar and Nayband, mostly due to the modalities’ life span of ≤1 year’s contribution of 4.5% and the modalities’ AMBI IV’s contribution of 4.35%. Sirik and Nayband had an average dissimilarity of 36.79% and a modality life span of ≤1 year (cont. = 7.34%) and size of 21–50 (cont. = 6.21%) contributed the most to this region division. The average dissimilarity between habitats (vegetated and mudflats) was 33.44%, and the average dissimilarity between seasons was 32.71% (winter and summer). The average dissimilarity was mostly explained by the modalities’ size of 21–50 and scavenger between habitats (size = 21–50, contribution = 4.66%, and scavenger cont. = 4.36%) and seasons (size = 21–50, cont. = 4.37%, and scavenger cont. = 4.26%) (Table S3).
Several environmental variables were measured in different regions and seasons, including TOC, TON, and silt/clay content sediments, salinity, and temperature (Table 3).
The contribution of environmental variables to variation in species and trait compositions of polychaetes for three regions was determined using the DistLM model (Table 4 and Table 5). Using the DISTLM routine, fitted values were then ordinated using a distance-based redundancy analysis (dbRDA) (Figure 5). The marginal test indicated that TOC significantly explained species variation in Sirik (p < 0.05), while environmental variables did not show significant differences in the other regions (p > 0.05). The Best model indicated that TOC (AIC = 314.1, R2 = 4.3531) in Gwadar, the combination of TON, TOC, and silt/clay (AIC = 238.73, R2 = 0.26977) in Sirik, and the combination of temperature and silt/clay (AIC = 31.688, R2 = 0.82016) in Nayband were the most powerful predictors of the variance in the polychaete community (Table 4).
The marginal test revealed that silt/clay in Sirik and salinity in Nayband each significantly explained the variation in the trait composition (p < 0.05). According to the Best model, the most important predictor variables of polychaete traits in mangrove ecosystems were the combination of temperature and TOC (AIC = 254.77, R2 = 0.15525) in Gwadar, the combination of TON, TOC, salinity, and temperature (AIC = 181.46, R2 = 0.39001) in Sirik, and the combination of temperature and salinity (AIC = 22.609, R2 = 0.89395) in Nayband (Table 5).
The first two axes of dbRDA of Gwadar explained 100% of the fitted variation and 4.4% of the total variation. The first dbRDA axis was mainly determined with TOC (dbRDA = 0.986), whereas the second axis was defined with TON and silt/clay (dbRDA = 0.856) (Figure 5a). In Sirik, the first two axes of dbRDA explained 96.8% of the total variation (dbRDA1 = 71.7% and dbRDA2 = 25.1%) (Figure 5b). The first axis was related to TOC (dbRDA = −0.786) and the second axis was defined with the combination of TON (dbRDA = 0.76) and silt/clay (dbRDA = −0.4) (Figure 5b). In Nayband, the first dbRDA axis was mainly determined with silt/clay (dbRDA = 0.926), while the second axis was defined with temperature (dbRDA = 0.926), which explained 100% of the fitted variation (dbRDA1 = 55.7% and dbRDA2 = 44.3%) (Figure 5c).
The dbRDA plots showed the contributors’ variables in the variation of the traits of the three regions. Temperature was the main predictor of the first axis (dbRDA = −0.775), while TOC was the main predictor of the second axis (dbRDA = 0.775), which explained 100% of the total variation in Gwadar (dbRDA = 88.9% and dbRDA = 11.1% dbRDA2) (Figure 6a). In Sirik, the first two dbRDA axes explained 97.6% of the total variation (dbRDA1 = 77.6% and dbRDA2 = 26%). The first axis was silt/clay (dbRDA = 0.785) and the second axis was the combination of TOC (dbRDA= 0.624), TON (dbRDA = 0.008), and temperature (dbRDA = −0.78) (Figure 6b). The first dbRDA axis of Nayband was mainly defined with salinity (dbRDA = 0.946) while the second axis was related to temperature (dbRDA = 0.946), which explained 100% of the total variation (dbRDA1 = 64.5% and dbRDA2 = 35.5%) (Figure 6c).

4. Discussion

In this study, the most abundant polychaete species belonged to the Nereididae, Capitellidae, and Paraonidae families in mangrove ecosystems (Table S1). Delfan et al. (2021) [23] reported that the most abundant polychaete families were Capitellidae and Nereididae, the latter being represented mostly by Perinereis horsti and Simplisetia qeshmensis in a mangrove ecosystem in the Persian Gulf. Hajalizadae et al. (2020) [22] stated that the most abundant polychaetes were Capitellidae and Nereididae in different mangrove habitats in the Persian Gulf. Nereididae are omnivores with jaws that can reduce the particle size of detritus [46]. Therefore, they can increase bioturbation and fragmentation in mangroves. Since capitellidae are deposit feeders, infunal, and burrowers, they play an essential role in cycling nutrients in estuaries [52].
The abundance and taxonomic diversity of polychaetes in our study were different in the three regions. The lowest richness and diversity indices were observed in the Nayband region. Mangroves of the Persian Gulf have less species richness and diversity due to higher salinity and temperature levels than tropical mangroves [23]. Therefore, the Nayband estuary is under stress from environmental variables, and the results of DistLM indicate that temperature is the most powerful predictor of the species composition of polychaete assemblages. In addition, the highest amount of organic carbon was obtained in the Nayband estuary. In this region, oil, gas, and petrochemical industries, as well as fishing activities and the movement of boats and barges, have led to the entry of a wide variety of pollutants. Additionally, the closure of a part of the Nayband estuary entrance has disrupted the tidal cycle and thus enriched organic matter [53]. Therefore, these stressors are limiting factors commonly known to restrict both the abundance and diversity of polychaete assemblages in the Nayband estuary.
The highest abundance of polychaetes was observed in Sirik, but it had less diversity compared to the Gowadar region. There are two tree species, viz. Avicennia marina and Rhizophora mucronata, that are in the Sirik region, whereas only A. marina is present in the other two regions. The presence of two species of trees enhances structural complexity, creating complex habitats [23,24,27]. The structural complexity of R. mucronata affects tidal currents, which ultimately affect sedimentation and sediment characteristics within mangrove forests. In addition, R. mucronata roots have a large volume and variety of interstitial spaces that might be advantageous for sheltering mobile organisms that can navigate through narrow gaps to avoid larger predators [34]. Therefore, these reasons may be the factors affecting the increase in the abundance of polychaetes in the Sirik region. Delfan et al. (2021) [23] proposed that the low richness of the macrofauna may be attributed to low mangrove tree diversity in the Persian Gulf. Overall, the difference in mangrove benthic communities depends on the latitude, mangrove species, estuary morphology, and presence or absence of fresh water in the region, evaporation, and other environmental variables, which can be the result of specific environmental and ecological forms of each community [36].
The abundance, Shannon diversity, and richness index showed seasonal variation, which is consistent with the results of Hajializadea et al. (2020) [22], in the mangroves of the Qeshm region in the Persian Gulf.
The results of our study showed that the abundance and taxonomic diversity of polychaetes increased from the mudflats to the vegetated habitats. Also, nMDS showed that species composition differed between habitats. We observed similar results to those of Checon et al. (2017) [29], Leung (2015) [31], and Pan et al. [5]. Many studies, including the present study, show that the presence of vegetation and structural components (such as roots, algal mats, and pneumatophores) significantly changed sediment characteristics. In the mangrove ecosystem, prop roots and pneumatophores helped trap detritus and litter, and also weakened wave action to increase sedimentation rates, which resulted in finer sediment and more organic matter [31,54]. The abundance and diversity indices decrease with the increase in root biomass, which may lead to inhibition and reduction in the burrowing behavior and feeding activity [5,31,55]. As reported by Leung (2015) [31] and Checon et al. (2017) [29], the density of polychaetes in the mangrove was lower than those in mudflats, and the density of polychaetes was negatively correlated with root biomass. There may be a difference in species composition of polychaete assemblages between vegetated and mudflats due to the distinct sedimentary characteristics, such as higher organic matter in a mudflat habitat [56], different temperatures, and the shelter provided by mangrove stand structures [32,57]. However, some studies showed that roots had positive effects on macrobenthos abundance and diversity because they enhanced sediment stability, the complexity of the habitat, and protected against predators [22,23,58,59].
There were several dominant trait modalities found in the three studied mangrove regions, including a large size, biodiffuser, burrower, crawler, life span of 1–3 years, and omnivore. Even though bioturbation is often regarded as a small-scale process, it plays an important role in the ecology of mangroves. For example, it can change the topography, increase oxygenation of deep sediment layers, affect community metabolism directly, alter organic content, rework sediments, reduce sediment density, and influence the fate of pollutants [52]. A bioturbation process is particularly relevant in areas with muddy sediments, low permeability, low oxygen concentrations, and high concentrations of contaminants (i.e., a reduced exchange of water between sediments and the water column). Polychaetes perform functions such as organic matter rotation, turbidity control, and stability through bioturbation [60]. In the studied mangrove forests, almost all polychaetes were classified as a biodiffuser, which is consistent with the results of Martins and Barros (2022) [52].
In this study, large-sized polychaetes were dominant in Gwadar and Sirik regions in both seasons, while small-sized polychaetes were dominant in Nayband and mudflat habitats. Body size is related to such circumstances as the food web structure, trophic levels, and energy flow in the ecosystem [61] and indicates disturbance, movement of organic matter, and biological interactions in a community [22]. The results of the present study are consistent with Delfan et al. (2021) [23]. It has been shown that large polychaetes are more effective at oxygenating sediments and cycling nutrients in mangrove ecosystems [62]. Posey (1987) [63] found that the maximum body size decreased as the number of pneumatophores increased, possibly because of dense root structures that inhibit the burrowing and feeding of large-sized species, while the maximum body size was larger in the mud habitat. We found that all regions and habitats were dominated by burrower and crawler trait modalities, but the crawler polychaetes increased slightly in vegetation habitats. Also in other studies, surface crawlers are frequently found in habitats with sandy sediments [64], bare mud, algal mats, and pneumatophores [22,31].
According to our findings, the predatory polychaetes were slightly higher in vegetated habitats and omnivorous polychaetes dominated all habitats and regions. Probably, the large variety and amount of food led to an increase in surface deposit feeders, which led to an increase in predatory species in vegetated habitats [65,66]. The increase in omnivorous polychaetes in mangrove ecosystems is due to the presence of Nereididae (i.e., N. glandicincta), which showed high abundance in all habitats and regions. The success of omnivores against other feeding strategies is due to their broad dietary flexibility, which allows them to adjust to environmental and resource fluctuations [67]. Therefore, they balance their diet as a result of feeding needs, food quality, and the availability of alternative foods [68].
Polychaete assemblages are affected by a variety of environmental variables, such as temperature, salinity, sediment type, and TOC and TON in sediments [69,70]. The results of DistLM showed that the TOC variable in Gwadar, silt/clay, TOC, and TON variables in Sirik, and temperature and silt/clay variables in Nayband contributed the most to the taxonomic structure of polychaetes. Carvalho et al. (2013) [71] stated that environmental processes controlling patterns of diversity could ultimately be taxon- as well as locality-specific. Shillabeer and Tapp (1989) [72] stated that the mangrove environment was much more dynamic than the purely marine environment and therefore, there is every possibility of variation in species occurrence. Our results are consistent with previous studies worldwide and indicate that sediment variables (e.g., sediment grain size, TOC, and TON) are more relevant than water column variables (temperature and salinity) in the distribution pattern of polychaetes [31,70,71,73,74].
In the present study, TOC, silt/clay, and salinity were the main drivers of polychaete traits in three mangrove ecosystems (the DistLM analysis), which can influence the difference in the expression of traits between mangrove ecosystems (Gwader, Sirik, and Nayband) and habitats (vegetated and mudflat). The results of this study are in accordance with Nasi et al. (2018) [12], which suggests that habitat and sediment descriptors, such as particles of sand or clay, and the depth of TOC and TON, are related to polychaete assemblages taxonomically and functionally. The study by Medeiros et al. (2021) [25] revealed that polychaetes’ functional nesting patterns were driven, regardless of seasonality, by salinity gradients throughout the year. Therefore, salinity determined the functional nestedness of the polychaete community. There were a number of functional traits present in the polychaete community that were nested subsets of those observed in higher salinity sites, where a wider range of categories of functional traits was encountered [25].
Along with other environmental variables, such as temperature or food availability, salinity may also affect polychaetes’ physiology, regeneration, and growth [74,75,76,77]. Arrighetti and Penchaszadeh (2010) [78] proposed that sediment characteristics, dissolved oxygen, and salinity were the main factors affecting polychaete communities. Kim et al. (2021) [79] stated that the sediment composition (gravel, sand, and clay) and hydrological variables (dissolved oxygen, depth, and temperature) were important factors in polychaete distribution patterns. Whether the environmental variables included in the analysis change this pattern structure solely or whether incorporating other variables in the analysis changes the observed polychaete pattern is difficult to determine [12,79,80]. It is important to examine how each set of environmental characteristics (such as salinity, particle size, and organic matter) and human impacts affect ecological functions.
In the current study, changes in the taxonomic and functional structure of polychaetes in mangrove ecosystems were observed, which showed that the species and trait composition of polychaetes depends on the structural complexity of the respective habitat. As reported by Van der Linden et al. (2016) [9], polychaetes exhibited higher taxonomic and functional diversity than molluscs, suggesting that in comparison with molluscs, they may be utilizing the resources more efficiently, resulting in changes in the functioning of the systems. An evaluation of the changes and relationships between traits and diverse mangrove habitats can lead to a better understanding of the functioning of these habitats and their effects on the environment.

5. Conclusions

According to the results, polychaetes were distributed differently in the three studied mangrove ecosystems, which may be due to their characteristics and the local environmental conditions of each ecosystem. It was found that the abundance and biodiversity of polychaetes in the mudflat habitats were higher than the vegetated habitats. Vegetated habitats had higher proportions of mobile (crawler) predatory species that are longer lived (3–5 years), with larger body size (80–100 mm), and are upward conveyors, whereas mudflat habitats had higher proportions of mobile (burrower) omnivore species that are moderately lived (1–3 years), with larger body size (>100 mm), and are a biodiffuser. These traits played important roles in determining the functional composition of a polychaete assemblage in mangrove ecosystems. The results showed that the species and trait composition of polychaetes in two habitats of a mangrove ecosystem is distinct, and the increasing abundance of polychaetes may be related to the structural complexity of their respective habitats. The advantage and importance of using taxonomic diversity and BTA of polychaetes and the impact of environmental variables on them generate information on how to respond and change the functioning of mangrove ecosystems, allowing us to make more efficient conclusions about biodiversity conservation and ecosystem functionality in future research.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/d15090998/s1, Table S1: Taxonomic list and mean density (ind.m−2 ± SE) of polychaetes in the mangrove vegetated habitats and mudflats in the Persian Gulf and Gulf of Oman; Table S2: Result of SIMPER analysis determining the major species leading to the polychaete community structure difference between regions (GT—Gwadar, SK—Sirik, and NY—Nyband), habitats (V—vegetated and M—mudflat), and seasons (W—winter and S—summer) in mangrove ecosystems in the Persian Gulf and Gulf of Oman (≥50% cumulative frequency); Table S3: Result of SIMPER analysis determining the major traits leading to the polychaete community structure difference between regions (GT—Gwadar, SK—Sirik, and NY—Nyband), habitats (V—vegetated and M—mudflat), and seasons (W—winter and S—summer) in mangrove ecosystems in the Persian Gulf and Gulf of Oman (≥50% cumulative frequency).

Author Contributions

Conceptualization, M.M., J.S. and M.G.S.; methodology, M.M., M.G.S. and H.R.; software, M.M., M.G.S. and M.V.; validation, J.S., M.G.S. and H.R.; formal analysis, M.M., J.S. and M.G.S.; investigation, M.M. and M.G.S.; data curation, M.M.; writing—original draft preparation, M.M. and M.G.S.; writing—review and editing, J.S., H.R. and M.V.; supervision, J.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Data Availability Statement

The datasets generated during and/or analyzed during the current study are available from the supplementary data files as well as from the corresponding author on reasonable request.

Acknowledgments

We gratefully acknowledge the financial support provided by the Department of Marine Biology, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Iran.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Positions of the sampling sites of polychaetes in Hara Biosphere Reserve, Persian Gulf and Gulf of Oman.
Figure 1. Positions of the sampling sites of polychaetes in Hara Biosphere Reserve, Persian Gulf and Gulf of Oman.
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Figure 2. Abundance and taxonomic diversity (mean ± SE) of polychaete assemblages in vegetated and mudflat habitats of different mangrove ecosystems (GT—Gwadar, SK—Sirik, and NY—Nayband) in the Persian Gulf and Gulf of Oman; (a) abundance, (b) Shannon Wiener diversity, (c) Margalef richness, (d) Pielou’s evenness, (e) Simpson dominance index.
Figure 2. Abundance and taxonomic diversity (mean ± SE) of polychaete assemblages in vegetated and mudflat habitats of different mangrove ecosystems (GT—Gwadar, SK—Sirik, and NY—Nayband) in the Persian Gulf and Gulf of Oman; (a) abundance, (b) Shannon Wiener diversity, (c) Margalef richness, (d) Pielou’s evenness, (e) Simpson dominance index.
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Figure 3. Community weighted mean (CWM) of trait modalities of polychaetes from different regions (GT—Gwadar, SK—Sirik, and NY—Nayband), habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman. Color codes represent the trait affiliation; individual bars represent the trait modality expression. For trait modalities’ labels, see Table 1.
Figure 3. Community weighted mean (CWM) of trait modalities of polychaetes from different regions (GT—Gwadar, SK—Sirik, and NY—Nayband), habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman. Color codes represent the trait affiliation; individual bars represent the trait modality expression. For trait modalities’ labels, see Table 1.
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Figure 4. nMDS ordination plots of species and trait composition of polychaetes across regions, habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman (GT—Gwadar, SK—Sirik, NY—Nayband, W—Winter, S—Summer, V—Vegetated, and M—Mudflat).
Figure 4. nMDS ordination plots of species and trait composition of polychaetes across regions, habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman (GT—Gwadar, SK—Sirik, NY—Nayband, W—Winter, S—Summer, V—Vegetated, and M—Mudflat).
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Figure 5. Distance-based redundancy analysis (dbRDA) ordination based on the best set of abiotic variables (using BEST as selection procedure and AIC as selection criterion) and polychaete species composition data from the three mangrove ecosystems in the Persian Gulf and Oman Gulf; (a) Gwadar, (b) Sirik, and (c) Nayband. The length and direction of vectors indicate the strength and direction of the relationship, respectively.
Figure 5. Distance-based redundancy analysis (dbRDA) ordination based on the best set of abiotic variables (using BEST as selection procedure and AIC as selection criterion) and polychaete species composition data from the three mangrove ecosystems in the Persian Gulf and Oman Gulf; (a) Gwadar, (b) Sirik, and (c) Nayband. The length and direction of vectors indicate the strength and direction of the relationship, respectively.
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Figure 6. Distance-based redundancy analysis (dbRDA) ordination based on the best set of abiotic variables (using BEST as selection procedure and AIC as selection criterion) and polychaete trait composition data from the three mangrove ecosystems in the Persian Gulf and Oman Gulf; (a) Gwadar, (b) Sirik, and (c) Nayband. The length and direction of vectors indicate the strength and direction of the relationship, respectively.
Figure 6. Distance-based redundancy analysis (dbRDA) ordination based on the best set of abiotic variables (using BEST as selection procedure and AIC as selection criterion) and polychaete trait composition data from the three mangrove ecosystems in the Persian Gulf and Oman Gulf; (a) Gwadar, (b) Sirik, and (c) Nayband. The length and direction of vectors indicate the strength and direction of the relationship, respectively.
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Table 1. List of polychaete traits and trait modalities used for the biological trait analysis. A label is given for each category.
Table 1. List of polychaete traits and trait modalities used for the biological trait analysis. A label is given for each category.
TraitModalitiesLabelDefinition
Size of organism (mm)5–10 mmSi.5The largest reported size for the species during adult stage.
10–20 mmSi.10
20–50 mmSi.20
50–80 mmSi.50
80–100 mmSi.80
>100 mmSi > 100
Ecosystem engineeringBiodiffuser Ec.biThe biodiffusers provide constant and random biomixing of sediment over short distances through their activities.
Upward conveyor Ec.upVertically oriented species that typically feed head-down at depth in the sediment. Vertically oriented head-down feeders actively select and ingest particles at the deeper sediments and egest these non-locally as feces in the sediment surface.
Downward conveyorEc.doDownward conveyors exhibit a feeding strategy opposite to that of upward conveyors.
RegeneratorEc.reThey mechanically transfer sediment from depth to surface by digging and maintaining burrows in the sediment.
Blind-ended ventilationEc.blFor breathing and feeding, animals flush their burrows with overlying water.
Open-ended ventilationEc.opOpen-ended ventilation consists of U-shaped burrows that can be flushed easily one after another.
Habitat buildingEc.haSpecies that create habitats for other species by building structures.
Feeding typePredator Fe.prA predatory organism that feeds by killing other organisms.
Suspension feederFe.suOrganisms that consume particulate organic matter in the water column, including plankton.
Non-selective deposit feederFe.nsAn organism that feeds on mud or sand and may show some discrimination in particle size or type.
Selective deposit feederFe.sdDeposit feeders do not ingest sediment haphazardly, but sort organic material from sediment using their palps or buccal organs.
Deposit feeder (selective or non-selective)Fe.dsnAn organism that feeds on fragmented particulate organic matter.
OmnivoreFe.omMixed-diet organisms that consume plant and animal material.
ScavengerFe.scAn organism that consumes dead animals actively.
HerbivoreFe.heAn organism that feeds on plants or algae, or parts of them.
Mobility of adultCrawler Mo.crMoving along the substratum by using its legs and appendages (e.g., parapodia and chaetae or muscles).
BurrowerMo.buBurrowing organisms that live and move in soft sediments.
SwimmerMo.swAn organism that moves through the water column using its fins, legs, or appendages.
Non-motile/Semi-motileMo.no
Life span (years)≤1 yearLif < 1Maximum length of time that any particular organism can be expected to live.
1–3 yearsLif.3
3–5 yearsLif.5
≥5 yearsLif > 5
Tolerance (AMBI index)Group I AB.IBased on the AMBI index, the sensitivity of an organism to organic enrichment.
Group II AB.II
Group IIIAB.III
Group IV AB.IV
Group VAB.V
Note: Trait modalities are defined based on the online database http://polytraits.lifewatchgreece.eu/, accessed on 25 March 2023.
Table 2. Results of three-factor ANOVA for comparing the abundance, taxonomic diversity, and CWM index of polychaetes across regions, habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman. Factors: region (levels: Gwadar, Sirik, and Nayband), habitat (levels: vegetated and mudflat), and season (levels: winter and summer); bold values indicate significant level.
Table 2. Results of three-factor ANOVA for comparing the abundance, taxonomic diversity, and CWM index of polychaetes across regions, habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman. Factors: region (levels: Gwadar, Sirik, and Nayband), habitat (levels: vegetated and mudflat), and season (levels: winter and summer); bold values indicate significant level.
FactorFp
Abundance (ind.m−2)Region2.280.002
Habitat (Region)6.330.000
Season1.90.024
Shannon–Wiener diversityRegion10.530.000
Habitat (Region)3.360.048
Season6.010.026
Margalef richnessRegion2.170.017
Habitat (Region)1.980.03
Season1.390.05
Pielou’s evennessRegion5.970.01
Habitat (Region)1.30.085
Season2.420.068
Simpson dominance Region13.590.000
Habitat (Region)2.230.12
Season2.650.09
CWM indexRegion2.220.000
Habitat (Region)2.930.002
Season1.710.28
Table 3. Variations in environmental data (mean ± SD) between regions, habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman (TOC = Total Organic Carbon; TON = Total Organic Nitrogen).
Table 3. Variations in environmental data (mean ± SD) between regions, habitats, and seasons in mangrove ecosystems in the Persian Gulf and Gulf of Oman (TOC = Total Organic Carbon; TON = Total Organic Nitrogen).
RegionWinterSummer
GwadarVegetatedMudflatVegetatedMudflat
TON (mg/g)0.14 ± 0.090.08 ± 0.040.12 ± 0.060.09 ± 0.008
TOC (mg/g)0.68 ± 0.30.35 ± 0.150.85 ± 0.40.58 ± 0.26
Silt/Clay (%)1.34 ± 0.540.93 ± 0.342.2 ± 1.5 1.67 ± 0.6
Temperature (°C)22.5 ± 0.7522.53 ± 0.7130.82 ± 0.3230.56 ± 1.85
Salinity (PPT)30.54 ± 0.3730.6 ± 0.3431 ± 0.4631.33 ± 0.49
SirikVegetatedMudflatVegetatedMudflat
TON (mg/g)0.025 ± 0.0010.019 ± 0.0090.03 ± 0.0080.01 ± 0.007
TOC (mg/g)0.78 ± 0.20.36 ± 0.150.75 ± 0.30.22 ± 0.1
Silt/Clay (%)2.12 ± 0.911.8 ± 12.13 ± 0.881.11 ± 0.61
Temperature (°C)23.8 ± 0.5423.45 ± 0.4430.8 ± 0.5131.48 ± 0.37
Salinity (PPT)30.68 ± 0.5230.86 ± 0.4532.3 ± 0.7431.87 ± 1
NaybandVegetatedMudflatVegetatedMudflat
TON (mg/g)0.08 ± 0.010.03 ± 0.0020.06 ± 0.0060.04 ± 0.003
TOC (mg/g)2.34 ± 0.181.76 ± 0.662.23 ± 0.21.49 ± 0.89
Silt/Clay (%)1.37 ± 0.580.88 ± 0.352.93 ± 1.481.05 ± 0.63
Temperature (°C)24.7 ± 0.5125.23 ± 0.232.74 ± 2.7433 ± 1.07
Salinity (PPT)28.72 ± 0.5229.1 ± 0.1430.57 ± 0.8530.04 ± 1.23
Table 4. Results of the DistLM analysis used to identify environmental variables influencing the species composition of polychaete assemblages in mangrove ecosystems (GT—Gwadar, SK—Sirik, and NY—Nayband) in the Persian Gulf and Gulf of Oman. p-Values were obtained from 9999 permutations of residuals under a reduced model. SS (trace) = portion of sum of squares related to the analyzed environmental variable; Pseudo-F = F value with permutation; bold values indicate significant level.
Table 4. Results of the DistLM analysis used to identify environmental variables influencing the species composition of polychaete assemblages in mangrove ecosystems (GT—Gwadar, SK—Sirik, and NY—Nayband) in the Persian Gulf and Gulf of Oman. p-Values were obtained from 9999 permutations of residuals under a reduced model. SS (trace) = portion of sum of squares related to the analyzed environmental variable; Pseudo-F = F value with permutation; bold values indicate significant level.
VariablesGTSKNY
SS (Trace)Pseudo-FpSS (Trace)Pseudo-FpSS (Trace)Pseudo-Fp
TON4338.11.15930.34788.11.57510.1912932.70.545551
TOC6052.91.63840.0510,6373.75750.0193396.10.660211
Silt/Clay3491.90.927360.56416.52.1520.1255010.71.15540.48
Temperature (°C)5742.41.55080.122539.9 0.814020.47154121.30850.49
Salinity (PPT)4504.81.20530.291202.30.379540.7615121.71.19640.5
GTSKNY
Overall best solutionAICR2VariablesAICR2VariablesAICR2Variables
314.14.3531TOC238.730.26977TON, TOC, silt/clay31.6880.820Temperature, silt/clay
314.129.2068TOC, temperature238.850.21625TOC, silt/clay32.2990.790Temperature, salinity
314.194.1298Temperature239.910.28942TON, TOC, silt/clay, salinity33.70.702Salinity, silt/clay
Table 5. Results of the DistLM analysis used to identify environmental variables influencing the trait composition of polychaete assemblages in mangrove ecosystems (GT—Gwadar, SK—Sirik, and NY—Nayband) in the Persian Gulf and Gulf of Oman. p-Values were obtained from 9999 permutations of residuals under a reduced model. SS (trace) = portion of sum of squares related to the analyzed environmental variable; Pseudo-F = F value with permutation; bold values indicate significant level.
Table 5. Results of the DistLM analysis used to identify environmental variables influencing the trait composition of polychaete assemblages in mangrove ecosystems (GT—Gwadar, SK—Sirik, and NY—Nayband) in the Persian Gulf and Gulf of Oman. p-Values were obtained from 9999 permutations of residuals under a reduced model. SS (trace) = portion of sum of squares related to the analyzed environmental variable; Pseudo-F = F value with permutation; bold values indicate significant level.
VariablesGTSKNY
SS (Trace)Pseudo-FpSS (Trace)Pseudo-FpSS (Trace)Pseudo-Fp
TON229.90.265940.764578.221.12840.332235.560.2171
TOC1838.72.24290.109924.141.8480.151336.830.3261
Silt/Clay361.360.419790.60524855.59260.0051208.82.0330.129
Temperature (°C)26353.30340.0651346.12.77540.0721188.71.9660.151
Salinity (PPT)617.630.723470.4978521.6950.1841321.52.4550.044
GTSKNY
Overall best solutionAICR2VariablesAICR2VariablesAICR2Variables
254.77 0.15525TOC, Temperature181.46 0.39001TON, TOC, Silt/Clay, Salinity22.6090.893 Temperature, Salinity
255.217.5335Temperature182.41 0.32704TO, Silt/Clay, Salinity25.7930.764 Temperature, Silt/Clay
255.4 0.16345TON, TOC, Temperature, Salinity 182.9 0.36004TON, Temperature, Silt/Clay, Salinity26.38 0.551 Salinity
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Miri, M.; Seyfabadi, J.; Ghodrati Shojaei, M.; Rahimian, H.; Valipour, M. Polychaete Diversity and Functional Trait Composition in Subtropical Mangrove Ecosystems. Diversity 2023, 15, 998. https://doi.org/10.3390/d15090998

AMA Style

Miri M, Seyfabadi J, Ghodrati Shojaei M, Rahimian H, Valipour M. Polychaete Diversity and Functional Trait Composition in Subtropical Mangrove Ecosystems. Diversity. 2023; 15(9):998. https://doi.org/10.3390/d15090998

Chicago/Turabian Style

Miri, Mohadeseh, Jafar Seyfabadi, Mehdi Ghodrati Shojaei, Hassan Rahimian, and Mohammad Valipour. 2023. "Polychaete Diversity and Functional Trait Composition in Subtropical Mangrove Ecosystems" Diversity 15, no. 9: 998. https://doi.org/10.3390/d15090998

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