An Integrative Approach for Identifying Quinquelaophonte (Harpacticoida, Laophontidae) Species from Korea with the Description of a New Species †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. DNA Extraction
2.3. Morphlogical Examination and Image Acquisition
2.4. Amplification and Phylogenetic Analysis
3. Results
3.1. Taxonomical Account
3.2. Quinquelaophonte sominer Kim & Lee, 2023 sp. nov.
3.3. Molecular Data and Phylogenetic Analysis
4. Discussion
4.1. Taxonomic Remarks
4.2. Difficulties of Morphological Analysis for Quinquelaophonte Species
4.3. Molecular Data and Phylogeny
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Specimen ID | GenBank Accession Number | References | |||
---|---|---|---|---|---|---|
18S | 28S | COX1 | CYTB | |||
Harpacticoida Sars G.O., 1903 | ||||||
Quinquelaophonte sominer sp. nov. | sed81-06 | OR656936 | OR656939 | OR659904 | OR667760 1 | this study |
sed81-07 | OR656937 | - | OR659905 | OR667761 | this study | |
sed81-08 | OR656938 | OR656940 | OR659906 | OR667762 | this study | |
Q. aurantius Charry, Wells, Smith, Stringer & Tremblay, 2019 | MA73574 | MH444815 | - | MH444814 | - | [9] |
Q. enormis Kim, Nam, & Lee, 2020 | paratype09 | - | - | MT416598 | MT422734 | [17] |
paratype10 | - | - | MT416599 | MT422735 | [17] | |
paratype11 | - | - | MT416600 | MT422736 | [17] | |
paratype12 | - | - | MT416601 | MT422737 | [17] | |
paratype13 | MT410708 | MT420735 | MT416602 | MT422738 | [17] | |
paratype16 | - | - | MT416603 | - | [17] | |
Paralaophonte congenera (Sars G.O., 1908) | LEGO-HAR027 | KR048738 | KR048877 | KR049011 | - | [34] |
Pseudonychocamptus spinifer Lang, 1965 | 97 | MF077714 | MF077863 | MF077898 | - | Khodami et al. 2 |
Lourinia armata (Claus, 1866) | LEGO-HAR030 | KR048739 | KR048877 | KT030278 | - | [34] |
Phyllopodopsyllus similis Kim & Lee, 2023 | sed86-01 | OP923229 | OP923708 | OP897045 | - | [26] |
sed86-02 | OP923230 | OP923709 | OP897046 | - | [26] | |
Calanoida Sars G.O., 1903 | ||||||
Spinocalanus aspinosus Park, 1970 | 361 | MF796503 | MF796484 | MF796470 | - | [35] |
Caudacalanus sp. | 352 | MF796505 | MF796486 | MF796472 | - | [35] |
Fragment | For ML | For BI | Length (bp) | Constant (bp) | Parsimony-Informative (bp) |
---|---|---|---|---|---|
18S | TIM + F + R2 | GTR + I + G | 1735 | 1423 | 229 |
28S | GTR + F + G4 | GTR + G | 792 | 501 | 231 |
COX1 | TIM + F + I + R3 | GTR + I + G | 500 | 259 | 210 |
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Kim, J.; Lee, W. An Integrative Approach for Identifying Quinquelaophonte (Harpacticoida, Laophontidae) Species from Korea with the Description of a New Species. Diversity 2023, 15, 1168. https://doi.org/10.3390/d15121168
Kim J, Lee W. An Integrative Approach for Identifying Quinquelaophonte (Harpacticoida, Laophontidae) Species from Korea with the Description of a New Species. Diversity. 2023; 15(12):1168. https://doi.org/10.3390/d15121168
Chicago/Turabian StyleKim, Jaehyun, and Wonchoel Lee. 2023. "An Integrative Approach for Identifying Quinquelaophonte (Harpacticoida, Laophontidae) Species from Korea with the Description of a New Species" Diversity 15, no. 12: 1168. https://doi.org/10.3390/d15121168