DNA Barcoding of Mullets (Family Mugilidae) from Pakistan Reveals Surprisingly High Number of Unknown Candidate Species
Abstract
1. Introduction
2. Materials and Methods
2.1. DNA Barcode Reference Library and Taxonomical Nomenclature
2.2. Sample Collection and Identification
2.3. DNA Amplification and Sequencing
2.4. DNA Barcode-Based Species Identification
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Morpho Species | Code No. | Location | Co-Ordinates | Accession No. |
---|---|---|---|---|
Mugil cephalus | 792 | Kakapir, Karachi | 24°50′42″ N 66°54′01″ E | MT943713 |
794 | Kakapir, Karachi | 2450′42″ N 66°54′01″ E | MT943714 | |
PMNH-55212 | Jiwani, Baluchistan | 25°10′59″ N 61°46′24″ E | MN511974 | |
PMNH-55368 | Gwadar, Baluchistan | 24°06′53″ N 62°19′41″ E | MN511975 | |
Planiliza macrolepis | PMNH-54728 | Ibrahim Haideri, Karachi | 24°47′39″ N 67°08′31″ E | MN512028 |
Planiliza subviridis | PAK Mu 851 | Somniani, Baluchistan | 25°09′25” N 66°43′25” E | MT943724 |
806 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943723 | |
840 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943722 | |
PMNH-55121 | Ibrahim Haideri, Karachi | 24°47′39″ N 67°08′31″ E | MN511966 | |
Planiliza klunzingeri | PAK Mu 884 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943743 |
PAK Mu 804 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943734 | |
824 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943727 | |
816 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943737 | |
830 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943735 | |
828 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943736 | |
PaK Mu 3 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943740 | |
PAK Mu 872 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943730 | |
PAK Mu 870 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943731 | |
PAK Mu 881 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943729 | |
PAK Mu 882 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943728 | |
PAK Mu 883 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943744 | |
802 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943742 | |
827 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943726 | |
811 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943739 | |
812 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943738 | |
832 | Gwadar, Baluchistan | 25°06′53″ N 62°19′41″ E | MT943725 | |
PAK Mu 821 | Gwadar, Baluchistan | 25°06′53″ N 62°19′41″ E | MT943733 | |
823 | Gwadar, Baluchistan | 25°06′53″ N 62°19′41″ E | MT943732 | |
PMNH 55125 | Gwadar, Baluchistan | 25°06′53″ N 62°19′41″ E | MN12027 | |
Crenimugil seheli | 819 | Gwadar, Baluchistan | 25°06′53″ N 62°19′41″ E | MT943705 |
PMNH-55231 | Gwadar, Baluchistan | 25°06′53″ N 62°19′41″ E | MN512029 | |
Osteomugil sp. | 829 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943716 |
PAK Mu 866 | Somniani, Baluchistan | 25°09′25” N 66°43′25” E | MT943715 | |
PMNH-55122 | Gwadar, Baluchistan | 25°06′53″ N 62°19′41″ E | MN512031 | |
810 | Karachi Fish harbor | 24°50′57″ N 67°27′10″ E | MT943717 | |
PAK Mu 864 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943718 | |
PAK Mu 862 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943719 | |
PAK Mu 2 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943721 | |
PAK MU 885 | Karachi Fish harbor | 24°50′57″ N 66°58′35″ E | MT943720 | |
Ellochelon vaigiensis | 805 | Keti Bunder, Sindh | 24°07′49″ N 67°27′10″ E | MT943712 |
PMNH-55070 | French beach, Karachi | 24°50′32″ N 66°48′53″ E | MN511887 |
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Hasan, A.; Siddiqui, P.J.A.; Amir, S.A.; Durand, J.-D. DNA Barcoding of Mullets (Family Mugilidae) from Pakistan Reveals Surprisingly High Number of Unknown Candidate Species. Diversity 2021, 13, 232. https://doi.org/10.3390/d13060232
Hasan A, Siddiqui PJA, Amir SA, Durand J-D. DNA Barcoding of Mullets (Family Mugilidae) from Pakistan Reveals Surprisingly High Number of Unknown Candidate Species. Diversity. 2021; 13(6):232. https://doi.org/10.3390/d13060232
Chicago/Turabian StyleHasan, Ariba, Pirzada Jamal Ahmed Siddiqui, Shabir Ali Amir, and Jean-Dominique Durand. 2021. "DNA Barcoding of Mullets (Family Mugilidae) from Pakistan Reveals Surprisingly High Number of Unknown Candidate Species" Diversity 13, no. 6: 232. https://doi.org/10.3390/d13060232
APA StyleHasan, A., Siddiqui, P. J. A., Amir, S. A., & Durand, J.-D. (2021). DNA Barcoding of Mullets (Family Mugilidae) from Pakistan Reveals Surprisingly High Number of Unknown Candidate Species. Diversity, 13(6), 232. https://doi.org/10.3390/d13060232