Next Article in Journal
Diversity of Feeding in Anthozoa (Cnidaria): A Systematic Review
Previous Article in Journal
Monitoring of Honey Bee Colony Losses: A Special Issue
Open AccessArticle

Genetic Differentiation of an Endangered Megalobrama terminalis Population in the Heilong River within the Genus Megalobrama

1
National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China
2
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
*
Authors to whom correspondence should be addressed.
Diversity 2020, 12(10), 404; https://doi.org/10.3390/d12100404
Received: 6 August 2020 / Revised: 5 September 2020 / Accepted: 15 October 2020 / Published: 18 October 2020
(This article belongs to the Special Issue Aquatic Restoration Ecology and Monitoring)
Megalobrama terminalis, which inhabits the Sino-Russian Heilong-Amur River Basin, has decreased critically since the 1960s. It has been listed in the Red Book of Endangered Fish Species by the Russian Federation in 2004. To guide the utilization and conservation programs of M. terminalis in the Heilong River (MTH), 3.1 kb of mitochondrial DNA (mtDNA) concatenated sequences and sequence-related amplified polymorphism (SRAP) markers (15 primer combinations) were applied to explore the genetic divergence and population differentiation of MTH within the genus Megalobrama. Clear genetic divergence between MTH and six other populations of the genus Megalobrama was found by haplotype network (mtDNA) and principal component (SRAP) analyses. Moreover, the STRUCTURE analysis based on SRAP data showed that MTH could be assigned to a particular cluster, whereas conspecific M. terminalis in the Qiantang River and Jinsha River Reservoir belonged to the same cluster. Analysis of molecular variance (AMOVA) and Fst statistics for the mtDNA and SRAP data revealed significant genetic variance and differentiation among all detected populations. Taken together, the results suggest that MTH has a strong genetic differentiation from other populations within the genus Megalobrama, which contributes to effective utilization in artificial cultivation and breeding of MTH. Furthermore, these results also provide a scientific basis for the management of MTH as a separate conservation unit. View Full-Text
Keywords: Megalobrama terminalis; genetic differentiation; population structure; mitochondrial DNA; SRAP Megalobrama terminalis; genetic differentiation; population structure; mitochondrial DNA; SRAP
Show Figures

Figure 1

MDPI and ACS Style

Hu, X.; Ma, B.; Li, C.; Jia, Z.; Jiang, X.; Ge, Y.; Tong, J.; Shi, L. Genetic Differentiation of an Endangered Megalobrama terminalis Population in the Heilong River within the Genus Megalobrama. Diversity 2020, 12, 404.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop