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Short Note

1-(1H-Benzimidazol-2-yl)-3-[5-(trichloromethyl)-1,3,4-oxadiazol-2-yl]propan-1-one

by
Mohd Rashid
1,
Asif Husain
1,*,
Ravinesh Mishra
1,
Niyaz Ahmed
2 and
Nadeem Ahmed Siddique
3
1
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India
2
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India
3
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India
*
Author to whom correspondence should be addressed.
Molbank 2012, 2012(3), M766; https://doi.org/10.3390/M766
Submission received: 2 June 2012 / Accepted: 17 July 2012 / Published: 23 July 2012

Abstract

:
The title compound, 1-(1H-benzimidazol-2-yl)-3-[5-(trichloromethyl)-1,3,4-oxadiazol-2-yl]propan-1-one (2) was synthesized successfully from 4-(1H-benzimidazol-2-yl)-4-oxobutanehydrazide (1) under microwave irradiation in good yield by reacting with trichloroacetic acid, and the structure of title compound was confirmed on the basis of IR, 1H-NMR, 13C-NMR, MS and CHN analyses results.

Graphical Abstract

The search for new anticancer agents having a heterocyclic nucleus continues worldwide at various laboratories [1] and from the reported literature, it can be concluded that benzimidazole heterocycles are of great importance in their biological as well as synthetic aspects of medicinal chemistry. Various substituted derivatives of the benzimidazole nucleus showed remarkable biological activity such as antitumor/antiproliferative/anticancer [2,3,4,5,6], anti-inflammatory [7], antiviral including anti-HIV [8], antibacterial [9], antifungal [10] antioxidant [11] and cysticidal activities [12]. Similarly, 1,3,4-oxadiazole is a class of heterocyclic compounds that have attracted significant interest in medicinal chemistry as they have a wide range of pharmaceutical and pharmacological applications including potential antitumor/antiproliferative/anticancer activities [13,14]. In view of these points, it was thought worthwhile to prepare a new type of compound that comprises both benzimidazole and 1,3,4-oxadiazole ring systems in the hope to obtain a promising anticancer agent.
The purity of the compound was checked by single-spot TLC [using silica gel G (Merck No. 5554) as stationary phase] with T/E/F (toluene/ethyl acetate/formic acid, 5:4:1) as the eluent and spots located by means of iodine vapors/UV light. The melting point was determined in an open capillary tube and is uncorrected. The final product was purified by recrystallization from ethanol. The spectral data of the title compound were found to be in full agreement with its postulated structure. Nuclear magnetic resonance spectra were recorded on a Bruker Spectrospin DPX-300 in DMSO-d6, chemical shift (δ) values are reported in parts per million (ppm) and coupling constants (J) in Hz using tetramethylsilane as internal reference. The mass spectrum was recorded on a LCMS/MS (Perkin-Elmer and LABINDIA, Applied Biosystem), model no. API 3000 presented as m/z. The IR spectrum was recorded on a FT/IR (Jasco, Japan), model No. 410. Elemental analyses were performed on a Perkin-Elmer 240 analyzer and values were found in the range of ±0.4% for each element analyzed (C, H and N).
The new compound was prepared as outlined in Figure 1. The IR spectrum of the compound showed a band at 3336 cm−1 for N-H. In the 1H-NMR spectrum, an exchangeable singlet signal appeared at δ 12.10 ppm corresponding to the N-H proton. The observed bands in the IR spectrum at around 1728 and 1666 cm−1 account for C=O and C=N. The chemical shift in the 13C-NMR spectrum at δ 173.47 and 155.8 could be accounted for C=O and C=N. The characteristic band at around 1342 cm−1 for N-N=C is indicative for the formation of the oxadiazole ring. The title compound showed two triplets at appropriate chemical shifts in the 1H-NMR spectrum at around δ 3.20 (J = 6.9 Hz) and δ 2.80 (J = 6.9 Hz) and the 13C-NMR spectrum shows signals at around δ 29.8 and 28.1 which could be assigned to two methylene groups (-CH2-CH2-) forming a linker chain through which the benzimidazole nucleus is attached to the oxadiazole ring, thus representing the back bone of this compound. The presence of doublet, triplet and double doublet signals at around δ 7.70, 7.40 and 7.20 (J = 8.1 Hz, J = 8.1 Hz and J = 8.1 Hz, J = 7.2 Hz) indicated three benzimidazole hydrogens. The signals in the 13C-NMR spectrum which appear at around δ 160.0 could be assigned to the oxadiazole ring carbons. The mass spectrum (ESI-MS) shows the presence of molecular ion peaks at 358 (M+), 360 ([M+2]+), 362 ([M+4]+), 364 ([M+6]+) due to the presence of three chlorine atoms. The starting material 4-(1H-benzimidazol-2-yl)-4-oxobutanoic acid hydrazide (1) was synthesized based on our previous work [15].

Synthesis of 1-(1H-benzimidazol-2-yl)-3-[5-(trichloromethyl)-1,3,4-oxadiazol-2-yl]propan-1-one (2)

A mixture of 4-(1H-benzimidazol-2-yl)-4-oxobutanoic acid hydrazide (1) (0.001 mol, 0.232 g), trichloroacetic acid (0.001 mol, 0.163 g) and POCl3 (5 mL) was placed into a microwave reaction vessel equipped with a magnetic stir bar. The reaction vessel was placed into the scientific microwave synthesizer and irradiated at a power level of 6 (60%, 420 W) for 9 min. After completion of reaction, checked by single-spot TLC (eluent: toluene/ethyl acetate/formic acid, 5:4:1), the reaction mixture was cooled and poured slowly onto crushed ice, then neutralized with sodium bicarbonate solution. A solid mass precipitated, which was filtered and washed with excess water to remove the inorganic components. The product was purified by recrystallization from ethanol.
Yield: 91%; m.p. 244–245 °C; Rf = 0.45; greenish amorphous solid.
IR (KBr) υmax (cm−1): 3336 (NH), 3105 (CH), 2974 (CH2), 1728 (C=O), 1666 (C=N), 1569 (C=C), 1342 (N-N=C), 1180 (C-O-C, asymmetric), 1029 (C-O-C, symmetric), 759 (C-Cl). 1H-NMR (300 MHz, DMSO-d6): δ 12.10 (s, 1H, NH, D2O exchangeable), 7.71 (d, 1H, J = 8.1 Hz, H-4-benzimidazole), 7.41 (t, 1H, J = 8.1 Hz, H-7-benzimidazole), 7.29 (dd, 2H, J = 8.1 Hz, J = 7.2 Hz, H-5,6-benzimidazole), 3.23 (t, 2H, J = 6.9 Hz, CH2), 2.87 (t, 2H, J = 6.9 Hz, CH2CO). 13C-NMR (75 MHz, DMSO-d6): δ 173.47 (C=O), 160.00 (C, oxadiazole), 155.86 (C=N), 133.29, 132.05, 129.69, 128.43, 123.14, 116.45 (Ar-C), 79.66 (CCl3), 29.83 (CH2, CH2CO), 28.13 (CH2). ESI-MS (m/z): 358 (M+), 360 ([M+2]+), 362 ([M+4]+), 364 ([M+6]+).
Anal. calcd. for C13H9Cl3N4O2: C, 43.42; H, 2.52; N, 15.58. Found: C, 43.45; H, 2.56; N, 15.63.

Supplementary materials

Supplementary File 1Supplementary File 2Supplementary File 3

Acknowledgements

We are thankful to University Grant Commission, New Delhi, Government of India, for financial assistance in the form of Major Research Project [file No. 36-107/2008 (SR)] and thanks are also to Jamia Hamdard (Hamdard University), New Delhi, India for providing necessary facilities for research work.

References

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Figure 1. Synthetic route to the title compound 2.
Figure 1. Synthetic route to the title compound 2.
Molbank 2012 m766 g001

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MDPI and ACS Style

Rashid, M.; Husain, A.; Mishra, R.; Ahmed, N.; Siddique, N.A. 1-(1H-Benzimidazol-2-yl)-3-[5-(trichloromethyl)-1,3,4-oxadiazol-2-yl]propan-1-one. Molbank 2012, 2012, M766. https://doi.org/10.3390/M766

AMA Style

Rashid M, Husain A, Mishra R, Ahmed N, Siddique NA. 1-(1H-Benzimidazol-2-yl)-3-[5-(trichloromethyl)-1,3,4-oxadiazol-2-yl]propan-1-one. Molbank. 2012; 2012(3):M766. https://doi.org/10.3390/M766

Chicago/Turabian Style

Rashid, Mohd, Asif Husain, Ravinesh Mishra, Niyaz Ahmed, and Nadeem Ahmed Siddique. 2012. "1-(1H-Benzimidazol-2-yl)-3-[5-(trichloromethyl)-1,3,4-oxadiazol-2-yl]propan-1-one" Molbank 2012, no. 3: M766. https://doi.org/10.3390/M766

APA Style

Rashid, M., Husain, A., Mishra, R., Ahmed, N., & Siddique, N. A. (2012). 1-(1H-Benzimidazol-2-yl)-3-[5-(trichloromethyl)-1,3,4-oxadiazol-2-yl]propan-1-one. Molbank, 2012(3), M766. https://doi.org/10.3390/M766

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