Are the Genes nadA and norB Involved in Formation of Aflatoxin G1?
Abstract
:1. Introduction
2. Results
2.1. Production of gene disruptants
2.2. TLC analyses of extracts of BN9ΔnadA, BN9ΔnorA and BN9ΔnorB
2.3. LC/MS analysis of transformant extracts
2.4. LC/MS analysis of BN9ΔnorA and BN9ΔnorB transformant extracts
2.5. Comparsion of NadA in A. parasiticus and A. flavus
3. Discussion
4. Experimental Section
4.1. Construction of gene knockout vectors
4.2. Thin layer chromatography (TLC) and liquid chromatography mass spectrometry (LC/MS)
4.3. Sequence comparison
5. Conclusions
Acknowledgments
References
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LC Peak | Retention time, min (relative peak height) | Mass/charge [M+H+] (relative peak intensity) | |
---|---|---|---|
A. BN9ΔnadA | Peak 1 | Peak 2 | |
1 | 3.7 (4) | 371 (100) | 363 (6) |
2 | 4.3 (69) | 329 (100) | 355 (15) |
3 | 4.5 (100) | 313 (100) | 329 (39) |
4
| 5.3 (2)
| 387 (100)
| 339 (24)
|
B. BN9ΔnadA-TLC | |||
1 | 4.1 (50) | 329 (100) | 387 (15) |
2 | 4.3 (100) | 329 (100) | 383 (7) |
3 | 5.0 (7) | 339 (100) | 291 (46) |
4
| 5.4 (20)
| 387 (100)
| |
C. BN9ΔnorB | |||
1 | 3.7 (23) | 363 (100) | 361 (18) |
2 | 4.2 (5) | 355 (100) | 371 (6) |
3 | 4.3 (23) | 329 (100) | 311 (7) |
4 | 4.5 (100) | 313 (100) | 327 (37) |
5
| 5.0 (3)
| 339 (100)
| |
D. BN9ΔnorA | |||
3 | 4.3 (100) | 329 (100) | 311 (9) |
4 | 4.4 (82) | 313 (100) | 329 (25) |
5
| 5.0 (20)
| 339 (100)
| 295 (5)
|
E. BN9control | |||
1 | 4.3 (100) | 329 (100) | 311 (6) |
2 | 4.5 (94) | 313 (100) | 285 (5) |
3 | 5.0 (16) | 339 (100) | 295 (2) |
© 2008 by MDPI This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Ehrlich, K.C.; Scharfenstein, L.L., Jr.; Montalbano, B.G.; Chang, P.-K. Are the Genes nadA and norB Involved in Formation of Aflatoxin G1? Int. J. Mol. Sci. 2008, 9, 1717-1729. https://doi.org/10.3390/ijms9091717
Ehrlich KC, Scharfenstein LL Jr., Montalbano BG, Chang P-K. Are the Genes nadA and norB Involved in Formation of Aflatoxin G1? International Journal of Molecular Sciences. 2008; 9(9):1717-1729. https://doi.org/10.3390/ijms9091717
Chicago/Turabian StyleEhrlich, Kenneth C., Leslie L. Scharfenstein, Jr., Beverly G. Montalbano, and Perng-Kuang Chang. 2008. "Are the Genes nadA and norB Involved in Formation of Aflatoxin G1?" International Journal of Molecular Sciences 9, no. 9: 1717-1729. https://doi.org/10.3390/ijms9091717