Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]
Abstract
:1. Introduction
2. Material and Methods
2.1. Microorganisms
2.2. Preparation of neem leaf extract (NLE)
2.3. Cultures conditions and extraction of citrinin
2.4. Mycelium inhibition test
2.5. Citrinin determination
2.6. Recovery Experiments
2.7. Statistical Analysis
3. Results and Discussion
4. Conclusions
Acknowledgments
References
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Isolate | b NLE (mg/ml) | Citrinin (μg)c spectrophotometric assay | Reduction (%) | Citrinin (ng)d HPLC analyses | Reduction (%) |
---|---|---|---|---|---|
K1 | 0.0 | 2.17 × 10−2 | - | 1.23 × 104 | - |
3.12 | 1.33 × 10−2 | 39.1% | 0.175 × 104 | 85.86% | |
6.25 | 1.1 × 10−2 | 49.4% | - | ||
12.5 | 0.8 × 10−2 | 60.9% | - | - | |
25.0 | 0.5 × 10−2 | 74.7% | - | - | |
50.0
| 0.3 × 10−2 | 83.9%
| -
| -
| |
K4 | 0.0 | 4.25 × 10−2 | - | 3.28 × 104 | - |
3.12 | 2.55 × 10−2 | 40% | 0.42 × 104 | 87.16% | |
6.25 | 1.80 × 10−2 | 56,5% | - | - | |
12.5 | 1.55 × 10−2 | 63,5% | - | - | |
25.0 | 1.03 × 10−2 | 75.9% | - | - | |
50.0
| 0.5 × 10−2 | 88,2%
| -
| -
| |
K8 | 0.0 | 6.3 × 10−2 | - | 10.7 × 104 | - |
3.12 | 3.0 × 10−2 | 53.7% | - | - | |
6.25 | 2.0 × 10−2 | 68.6% | 0.55 × 104 | 94.86% | |
12.5 | 1.6 × 10−2 | 75.0% | - | - | |
25.0 | 1.0 × 10−2 | 84.0% | - | - | |
50.0 | 0.5 × 10−2 | 92.0% | - | - |
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Mossini, S.A.G.; Kemmelmeier, C. Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]. Int. J. Mol. Sci. 2008, 9, 1676-1684. https://doi.org/10.3390/ijms9091676
Mossini SAG, Kemmelmeier C. Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]. International Journal of Molecular Sciences. 2008; 9(9):1676-1684. https://doi.org/10.3390/ijms9091676
Chicago/Turabian StyleMossini, Simone Aparecida Gallerani, and Carlos Kemmelmeier. 2008. "Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]" International Journal of Molecular Sciences 9, no. 9: 1676-1684. https://doi.org/10.3390/ijms9091676