Next Article in Journal / Special Issue
Voltammetric Behavior of o-Nitrophenol and Damage to DNA
Previous Article in Journal
Closing in on Chemical Bonds by Opening up Relativity Theory
Previous Article in Special Issue
Selenium Derivatization of Nucleic Acids for Phase and Structure Determination in Nucleic Acid X-ray Crystallography
Open AccessArticle

RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras

1
Department of Life Sciences, Second University of Naples, Via Vivaldi 43, I-81100 Caserta, Italy
2
Department of Environmental Sciences, Second University of Naples, Via Vivaldi 43, I-81100 Caserta, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2008, 9(3), 299-315; https://doi.org/10.3390/ijms9030299
Received: 13 December 2007 / Revised: 29 February 2008 / Accepted: 29 February 2008 / Published: 12 March 2008
(This article belongs to the Special Issue Nucleic Acid Derivatives in Emerging Technologies)
The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3’-PNA chimeras on siRNA functioning and stability. We demonstrate that the chemical modifications are compatible with the siRNA machinery, because all the PNA-modified siRNAs can efficiently mediate specific gene silencing in mammalian cells. Furthermore, we find that the modification on the sense strand of siRNA results in an increased persistence of the activity, whereas modification on both strands results in enhanced nuclease resistance in serum. View Full-Text
Keywords: modified siRNA; RNA interference; RNA-PNA chimeras; Peptide Nucleic Acids; RNA. modified siRNA; RNA interference; RNA-PNA chimeras; Peptide Nucleic Acids; RNA.
Show Figures

MDPI and ACS Style

Potenza, N.; Moggio, L.; Milano, G.; Salvatore, V.; Di Blasio, B.; Russo, A.; Messere, A. RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras. Int. J. Mol. Sci. 2008, 9, 299-315.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop