Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation and surface modification of the SPMP microbeads
2.2. Application in the detection of free hCGβ
2.3. Optimization of the operation parameters of the sandwich magnetic ECLIA of free hCGβ
2.4. Sensitivity and recovery rate
2.5. Comparison between the results of clinical samples, by ECLIA and ELISA.
3. Conclusions
4. Experimental Section
4.1. General
4.2. Preparation of Oleic Acid-coating Fe3O4 Magnetic Fluid (1)
4.3. Synthesis of Poly(MA-DVB) SPMP microspheres (2)
4.4. Surface Modification of Poly(MA-DVB) SPMP microspheres with EDA(3)
4.5. Surface functionalization and activation (4)
4.6. Combination of anti-FITC (5)
4.7. Protocols of the whole assay of ECLIA (6)
4.8. Collection of the clinical serum samples (7)
Acknowledgements
References and Notes
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Guo, X.; Guan, Y.; Yang, B.; Wang, Y.; Lan, H.; Shi, W.; Yang, Z.; Lu, Z. Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads. Int. J. Mol. Sci. 2006, 7, 274-288. https://doi.org/10.3390/i7080274
Guo X, Guan Y, Yang B, Wang Y, Lan H, Shi W, Yang Z, Lu Z. Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads. International Journal of Molecular Sciences. 2006; 7(8):274-288. https://doi.org/10.3390/i7080274
Chicago/Turabian StyleGuo, Xiaoying, Yueping Guan, Bin Yang, Yongning Wang, Hualong Lan, Wentang Shi, Zhenghui Yang, and Zuhong Lu. 2006. "Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads" International Journal of Molecular Sciences 7, no. 8: 274-288. https://doi.org/10.3390/i7080274