SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A
Abstract
1. Introduction
2. Results
2.1. Increase in Lysine Succinylation in HUVECs Under Simulated Microgravity
2.2. Identification of Lysine-Succinylated Proteins and Sites in HUVECs Under Simulated Microgravity
2.3. Functional Enrichment Analysis of Lysine-Succinylated Proteins
2.4. Motif Analysis of Lysine-Succinylated Proteins
2.5. SIRT5 Is Downregulated and Regulates Succinylation Modification Levels of HUVECs Under Simulated Microgravity
2.6. SIRT5 Protects HUVECs from Apoptosis Induced by Simulated Microgravity
2.7. Simulated Microgravity Increases the Succinylation of ERO1A Protein at Site K396
2.8. Knockdown of SIRT5 Induces Apoptosis of HUVECs Through the Succinylation of Lys396 in ERO1A
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Weightless Model Establishment
4.2. Cell Transfection and Treatment
4.3. Protein Extraction and Tryptic Digestion
4.4. Lysine-Succinylated Peptide Affinity Enrichment
4.5. LC-MS/MS
4.6. Database Search
4.7. Bioinformatic Analysis
4.8. Western Blot Assay
4.9. Coimmunoprecipitation (Co-IP)
4.10. Apoptosis Assay
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pan, Y.; Zhang, Q.; Li, C.; Li, X.; Li, S.; Wang, Y.; Wang, R.; Fan, J.; Tie, Y.; Zhao, X.; et al. SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A. Int. J. Mol. Sci. 2025, 26, 2908. https://doi.org/10.3390/ijms26072908
Pan Y, Zhang Q, Li C, Li X, Li S, Wang Y, Wang R, Fan J, Tie Y, Zhao X, et al. SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A. International Journal of Molecular Sciences. 2025; 26(7):2908. https://doi.org/10.3390/ijms26072908
Chicago/Turabian StylePan, Yikai, Qian Zhang, Chengfei Li, Xi Li, Shuhan Li, Yuan Wang, Ruonan Wang, Jieyi Fan, Yateng Tie, Xingcheng Zhao, and et al. 2025. "SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A" International Journal of Molecular Sciences 26, no. 7: 2908. https://doi.org/10.3390/ijms26072908
APA StylePan, Y., Zhang, Q., Li, C., Li, X., Li, S., Wang, Y., Wang, R., Fan, J., Tie, Y., Zhao, X., Gao, Y., Wang, Y., & Sun, X. (2025). SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A. International Journal of Molecular Sciences, 26(7), 2908. https://doi.org/10.3390/ijms26072908