Lactobacillus plantae Expressing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Single-Chain Antibody Can Inhibit PRRSV Replication and Change the Intestinal Flora Structure of Piglets
Abstract
1. Introduction
2. Results
2.1. Lactobacillus plantarum Anchors and Expresses PRRSV Single-Chain Antibody and Prokaryotic Expression
2.2. Antiviral Effect in Vitro
2.3. Changes in Clinical Characterization of Experimental Pigs After Challenge
2.4. Poison Attack Pigs’ Autopsy Results After the Experiment
2.5. Virus Quantification Results of Each Tissue and Organ
2.6. Results of Viral Load Determination in Serum After Challenge
2.7. ELISA Analysis
2.8. Localization of Virus in Tissues via Immunofluorescence
2.9. Histopathological Observation
2.10. Flow Cytometry of Peripheral Blood T Lymphocytes After Challenge
2.11. Analysis of Comparative Results of Intestinal Flora
2.12. Analysis of Transcriptome Comparison Results
3. Discussion
4. Materials and Methods
4.1. Strains and Preservation
4.2. Construction of Recombinant Lactobacillus plantarum
4.3. Western Blot
4.4. PRRSV Titer Determination
4.5. Cytotoxicity Test
4.6. Three Groups of Proteins Against PRRSV Effect Research
4.7. Quantification of Virions in Cells
4.8. Design and Grouping of Animal Experiments
4.9. Flow Cytometry
4.10. Pathological Anatomical Observation
4.11. Hematoxylin–Eosin Staining
4.12. Enzyme-Linked Immunosorbent Assay
4.13. Indirect Immunofluorescence
4.14. Quantification of Virus Particles in Various Tissues and Organs
4.15. Intestinal Microbiota Sequencing
4.16. Transcriptome Sequencing
4.17. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Niu, T.; Fan, T.; Wang, Y.; Gao, K.; Zhao, J.; Wang, R.; Chen, X.; Xing, J.; Qiu, J.; Zou, B.; et al. Lactobacillus plantae Expressing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Single-Chain Antibody Can Inhibit PRRSV Replication and Change the Intestinal Flora Structure of Piglets. Int. J. Mol. Sci. 2025, 26, 2257. https://doi.org/10.3390/ijms26052257
Niu T, Fan T, Wang Y, Gao K, Zhao J, Wang R, Chen X, Xing J, Qiu J, Zou B, et al. Lactobacillus plantae Expressing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Single-Chain Antibody Can Inhibit PRRSV Replication and Change the Intestinal Flora Structure of Piglets. International Journal of Molecular Sciences. 2025; 26(5):2257. https://doi.org/10.3390/ijms26052257
Chicago/Turabian StyleNiu, Tianming, Tianqi Fan, Yingjie Wang, Kuipeng Gao, Jinhui Zhao, Ruyu Wang, Xiaolei Chen, Junhong Xing, Jingjing Qiu, Boshi Zou, and et al. 2025. "Lactobacillus plantae Expressing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Single-Chain Antibody Can Inhibit PRRSV Replication and Change the Intestinal Flora Structure of Piglets" International Journal of Molecular Sciences 26, no. 5: 2257. https://doi.org/10.3390/ijms26052257
APA StyleNiu, T., Fan, T., Wang, Y., Gao, K., Zhao, J., Wang, R., Chen, X., Xing, J., Qiu, J., Zou, B., Fan, S., Zhang, S., Wu, Q., Yang, G., Wang, N., Zeng, Y., Cao, X., Jiang, Y., Wang, J., ... Wang, C. (2025). Lactobacillus plantae Expressing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Single-Chain Antibody Can Inhibit PRRSV Replication and Change the Intestinal Flora Structure of Piglets. International Journal of Molecular Sciences, 26(5), 2257. https://doi.org/10.3390/ijms26052257