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Open AccessArticle
Magnesium Preserves Calcium Homeostasis and Contributes to Protect Myotubes from Inflammation-Induced Damage
1
Dipartimento di Medicina e Chirurgia Traslazionale, Fondazione Policlinico Universitario A. Gemelli IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy
2
IRCCS Neuromed, 86077 Pozzilli, Italy;
3
Dipartimento di Scienze Biomediche e Cliniche, Università di Milano, 20157 Milan, Italy
4
Departmental Faculty of Medicine, UniCamillus, Saint Camillus International University of Medical Sciences, 00131 Rome, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(20), 9912; https://doi.org/10.3390/ijms26209912 (registering DOI)
Submission received: 5 September 2025
/
Revised: 3 October 2025
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Accepted: 9 October 2025
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Published: 11 October 2025
Abstract
Magnesium (Mg2+) is a key regulator of cellular biochemical processes and an essential cofactor in skeletal muscle physiology. Although Mg2+ deficiency has been linked to reduced muscle strength, its role in the regulation of calcium (Ca2+) signaling and in inflammation remains incompletely understood. In this study, we examined the effects of Mg2+ availability using the murine myoblast cell line C2C12. Cells were differentiated under low, normal, or high Mg2+ conditions, and myotube formation, intracellular Ca2+ fluxes, and resistance to inflammatory stimuli were assessed. Mg2+ deficiency impaired myotube differentiation, while Mg2+ supplementation preserved Ca2+ response during stimulation and contributed to protect myotubes against inflammation-induced damage. Collectively, these findings highlight a dual role of Mg2+ in sustaining functional performance under repeated stress and protecting myotubes against inflammatory injury. This study supports the importance of adequate dietary Mg2+ intake as a potential strategy to mitigate muscle loss associated with aging and chronic inflammation.
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MDPI and ACS Style
Pietropaolo, G.; Castiglioni, S.; Maier, J.A.; Wolf, F.I.; Trapani, V.
Magnesium Preserves Calcium Homeostasis and Contributes to Protect Myotubes from Inflammation-Induced Damage. Int. J. Mol. Sci. 2025, 26, 9912.
https://doi.org/10.3390/ijms26209912
AMA Style
Pietropaolo G, Castiglioni S, Maier JA, Wolf FI, Trapani V.
Magnesium Preserves Calcium Homeostasis and Contributes to Protect Myotubes from Inflammation-Induced Damage. International Journal of Molecular Sciences. 2025; 26(20):9912.
https://doi.org/10.3390/ijms26209912
Chicago/Turabian Style
Pietropaolo, Giuseppe, Sara Castiglioni, Jeanette A. Maier, Federica I. Wolf, and Valentina Trapani.
2025. "Magnesium Preserves Calcium Homeostasis and Contributes to Protect Myotubes from Inflammation-Induced Damage" International Journal of Molecular Sciences 26, no. 20: 9912.
https://doi.org/10.3390/ijms26209912
APA Style
Pietropaolo, G., Castiglioni, S., Maier, J. A., Wolf, F. I., & Trapani, V.
(2025). Magnesium Preserves Calcium Homeostasis and Contributes to Protect Myotubes from Inflammation-Induced Damage. International Journal of Molecular Sciences, 26(20), 9912.
https://doi.org/10.3390/ijms26209912
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