A Haplotype GWAS in Syndromic Familial Colorectal Cancer
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsA Haplotype GWAS in Syndromic Familial Colorectal Cancer, attempts to confirm earlier GWAS findings for CRC associated with familial prostate or gastric cancer. The study is straight-forward GWAS. One problem I have is lack of definition of statistical significance levels for each analysis. In sections 2.1 and 2.2, what are the cut-offs? I was thinking that 0.001 would be appropriate based on 50 loci. One feature for the association analysis might be to compare haplotypes between the 293 cases with other cancers in families and the remaining cases with just CRC. This is not to reveal necessarily significant differences, but to another way to suggest CRC specific vs. syndromic variants in the absence of looking at family members with other cancers. In connection with this, it is mentioned that haplotypes found were associated with CRC and other cancers, specifically, were any unique?
Author Response
Please see the attachment
Author Response File: Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsAbstract
The aim should be more clearly identified
Introduction
The format is very strange
Additionally the aim of the study was not clear enough
4. Materials and Methods
4.1. Cases and Controls for GWAS
As the cancer is heredity disease so, the study should take in consideration the nationality of the included patient
Many steps in the method was depend on previously studies so the data is not clear and the author need a new paraphrases to the sentences to be more identified.
4.5. Genotyping, quality control and haplotype GWAS
The extracted and the method of identification is missing
Results Written is good
Discussion seemed as a new result written with no clear discussion
Tables has many abbreviation with no clear ligands
Absence of figures and diagrams explain the study or inclusion and exclusion criteria
References is good
Conclusion is poor with lack correlation with the results and discussion
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for AuthorsThe authors have addressed my comments satisfactorily.
Reviewer 2 Report
Comments and Suggestions for AuthorsThe authors succeeded in answering all the reviewer commends