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Article

Combining Time-Restricted Wheel Running and Feeding During the Light Phase Increases Running Intensity Under High-Fat Diet Conditions Without Altering the Total Amount of Daily Running

1
Netherlands Institute for Neuroscience, Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW), Meibergdreef 47, 1105BA Amsterdam, The Netherlands
2
Laboratory of Endocrinology, Department of Laboratory Medicine, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands
3
Amsterdam Gastroenterology, Endocrinology and Metabolism, 1105AZ Amsterdam, The Netherlands
4
Department of Endocrinology and Metabolism, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands
5
Brain Plasticity Group, Swammerdam Institute for Life Sciences, Faculty of Science, Science Park 904, 1098XH Amsterdam, The Netherlands
6
Centre for Urban Mental Health, University of Amsterdam, 1098XH Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(15), 7658; https://doi.org/10.3390/ijms26157658 (registering DOI)
Submission received: 6 June 2025 / Revised: 29 July 2025 / Accepted: 1 August 2025 / Published: 7 August 2025
(This article belongs to the Special Issue Molecular Research on Diabetes and Obesity)

Abstract

Excess caloric intake and insufficient physical activity are the two major drivers underlying the global obesity and type 2 diabetes mellitus epidemics. However, circadian misalignment of caloric intake and physical activity, as commonly experienced by nightshift workers, can also have detrimental effects on body weight and glucose homeostasis. We have previously reported that combined restriction of eating and voluntary wheel running to the inactive phase (i.e., a rat model for circadian misalignment) shifted liver and muscle clock rhythms by ~12 h and prevented the reduction in the amplitude of the muscle clock oscillation otherwise induced by light-phase feeding. Here, we extended on these findings and investigated how a high-fat diet (HFD) affects body composition and liver and muscle clock gene rhythms in male Wistar rats while restricting both eating and exercise to either the inactive or active phase. To do this, we used four experimental conditions: sedentary controls with no wheel access on a non-obesogenic diet (NR), sedentary controls with no wheel access on an HFD (NR-H), and two experimental groups on an HFD with simultaneous access to a running wheel and HFD time-restricted to either the light phase (light-run-light-fed + HFD, LRLF-H) or the dark phase (dark-run-dark-fed + HFD. DRDF-H). Consumption of an HFD did not alter the daily running distance of the time-restricted groups but did increase the running intensity in the LRLF-H group compared to a previously published LRLF chow fed group. However, no such increase was observed for the DRDF-H group. LRLF-H ameliorated light phase-induced disturbances in the soleus clock more effectively than under chow conditions and had a protective effect against HFD-induced changes in liver clock gene expression. Together with (our) previously published results, these data suggest that eating healthy and being active at the wrong time of the day can be as detrimental as eating unhealthy and being active at the right time of the day.
Keywords: circadian misalignment; time restricted running; time restricted feeding; liver; muscle; high fat diet; plin5 circadian misalignment; time restricted running; time restricted feeding; liver; muscle; high fat diet; plin5

Share and Cite

MDPI and ACS Style

Shiba, A.; Tandari, R.; Foppen, E.; Yi, C.-X.; Mul, J.D.; Stenvers, D.J.; Kalsbeek, A. Combining Time-Restricted Wheel Running and Feeding During the Light Phase Increases Running Intensity Under High-Fat Diet Conditions Without Altering the Total Amount of Daily Running. Int. J. Mol. Sci. 2025, 26, 7658. https://doi.org/10.3390/ijms26157658

AMA Style

Shiba A, Tandari R, Foppen E, Yi C-X, Mul JD, Stenvers DJ, Kalsbeek A. Combining Time-Restricted Wheel Running and Feeding During the Light Phase Increases Running Intensity Under High-Fat Diet Conditions Without Altering the Total Amount of Daily Running. International Journal of Molecular Sciences. 2025; 26(15):7658. https://doi.org/10.3390/ijms26157658

Chicago/Turabian Style

Shiba, Ayano, Roberta Tandari, Ewout Foppen, Chun-Xia Yi, Joram D. Mul, Dirk Jan Stenvers, and Andries Kalsbeek. 2025. "Combining Time-Restricted Wheel Running and Feeding During the Light Phase Increases Running Intensity Under High-Fat Diet Conditions Without Altering the Total Amount of Daily Running" International Journal of Molecular Sciences 26, no. 15: 7658. https://doi.org/10.3390/ijms26157658

APA Style

Shiba, A., Tandari, R., Foppen, E., Yi, C.-X., Mul, J. D., Stenvers, D. J., & Kalsbeek, A. (2025). Combining Time-Restricted Wheel Running and Feeding During the Light Phase Increases Running Intensity Under High-Fat Diet Conditions Without Altering the Total Amount of Daily Running. International Journal of Molecular Sciences, 26(15), 7658. https://doi.org/10.3390/ijms26157658

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