Efficient Genome Editing Using the T2A-Coupled Co-Expression of Two ZFN Monomers
Abstract
1. Introduction
2. Results
2.1. Design of ZF-ND1 Expression
2.2. T2A Peptide Enables Efficient Genome Editing with a Single Plasmid
2.3. Efficient Genome Editing with a Single Plasmid Encoding Other ZF-ND1s
2.4. Lower Doses of ZF-ND1 Plasmids and Another Cell Line
3. Discussion
4. Materials and Methods
4.1. ZF Design
4.2. Vector Construction
4.3. Cell Culture, DNA Transfection, and DNA Electroporation
4.4. Transient Cold Shock
4.5. T7EI Assay
4.6. Off-Target Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Katayama, S.; Yamamoto, T. Efficient Genome Editing Using the T2A-Coupled Co-Expression of Two ZFN Monomers. Int. J. Mol. Sci. 2025, 26, 7602. https://doi.org/10.3390/ijms26157602
Katayama S, Yamamoto T. Efficient Genome Editing Using the T2A-Coupled Co-Expression of Two ZFN Monomers. International Journal of Molecular Sciences. 2025; 26(15):7602. https://doi.org/10.3390/ijms26157602
Chicago/Turabian StyleKatayama, Shota, and Takashi Yamamoto. 2025. "Efficient Genome Editing Using the T2A-Coupled Co-Expression of Two ZFN Monomers" International Journal of Molecular Sciences 26, no. 15: 7602. https://doi.org/10.3390/ijms26157602
APA StyleKatayama, S., & Yamamoto, T. (2025). Efficient Genome Editing Using the T2A-Coupled Co-Expression of Two ZFN Monomers. International Journal of Molecular Sciences, 26(15), 7602. https://doi.org/10.3390/ijms26157602