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Article

Screening Method for the Selection of Oleaginous Yeast-Producing Gold Nanoparticles

by
Jesus D. Guerra
1,†,
Diana Mariscal-Nava
1,†,
Miguel Avalos-Borja
2 and
Georgina Sandoval
1,*
1
LIBBA Laboratory, Industrial Biotechnology Unit, Research and Assistance Center in Technology and Design of the State of Jalisco, A.C., Av. Normalistas No. 800 Col. Colimas de la Normal, C.P., Guadalajara 44270, Jalisco, Mexico
2
Advanced Materials Division, Potosin Institute of Scientific and Technological Research, Camino a la Presa de San José 2055, Lomas 4ta Secc, San Luis Potosí 78216, Mexico
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2025, 26(15), 7534; https://doi.org/10.3390/ijms26157534 (registering DOI)
Submission received: 27 June 2025 / Revised: 31 July 2025 / Accepted: 31 July 2025 / Published: 4 August 2025

Abstract

The demand for eco-friendly nanomaterial synthesis has increased interest in biological approaches. Yeast-mediated biosynthesis of gold nanoparticles (AuNPs) offers a sustainable alternative with potential biotechnological applications. This study developed a rapid screening method to identify oleaginous yeast strains able to synthesize AuNPs. A collection of 114 oleaginous yeasts from the LIBBA laboratory was screened. UV–Vis spectroscopy at 530–560 nm was used to assess nanoparticle formation, identifying 20 strains that effectively synthesize AuNP. Electron microscopy confirmed the presence of intracellular and extracellular nanoparticles, with variations in size and morphology. This screening and optimization approach effectively identified promising yeast candidates and refined biosynthesis conditions, providing a foundation for industrial-scale nanoparticle production.
Keywords: gold nanoparticles; lipolytic yeast; screening; UV–Vis spectroscopy; microbial nanotechnology; biosynthesis gold nanoparticles; lipolytic yeast; screening; UV–Vis spectroscopy; microbial nanotechnology; biosynthesis

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MDPI and ACS Style

Guerra, J.D.; Mariscal-Nava, D.; Avalos-Borja, M.; Sandoval, G. Screening Method for the Selection of Oleaginous Yeast-Producing Gold Nanoparticles. Int. J. Mol. Sci. 2025, 26, 7534. https://doi.org/10.3390/ijms26157534

AMA Style

Guerra JD, Mariscal-Nava D, Avalos-Borja M, Sandoval G. Screening Method for the Selection of Oleaginous Yeast-Producing Gold Nanoparticles. International Journal of Molecular Sciences. 2025; 26(15):7534. https://doi.org/10.3390/ijms26157534

Chicago/Turabian Style

Guerra, Jesus D., Diana Mariscal-Nava, Miguel Avalos-Borja, and Georgina Sandoval. 2025. "Screening Method for the Selection of Oleaginous Yeast-Producing Gold Nanoparticles" International Journal of Molecular Sciences 26, no. 15: 7534. https://doi.org/10.3390/ijms26157534

APA Style

Guerra, J. D., Mariscal-Nava, D., Avalos-Borja, M., & Sandoval, G. (2025). Screening Method for the Selection of Oleaginous Yeast-Producing Gold Nanoparticles. International Journal of Molecular Sciences, 26(15), 7534. https://doi.org/10.3390/ijms26157534

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