Membrane Progesterone Receptor Beta Regulates the Decidualization of Endometrial Stromal Cells in Women with Endometriosis
Abstract
1. Introduction
2. Results
2.1. Demographical and Clinical Data
2.2. Decidualization of Human ESCs
2.3. mPRs Participation in the Decidualization of Human Endometrial Stromal Cells
2.4. mPRβ Induces the Decidualization of Human Endometrial Stromal Cells
3. Discussion
4. Materials and Methods
4.1. Tissue Collection
4.2. Primary Cell Culture of ESCs
4.3. Cell Lines
4.4. Decidualization Treatments
4.5. RNA Isolation and Reverse Transcription-Quantitative PCR (RT-qPCR)
4.6. Immunofluorescence
4.7. mPRβ siRNA Transfection
4.8. Western Blot
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
mPRβ | Membrane Progesterone Receptor β |
ESCs | Endometrial Stromal Cells |
HAND2 | Heart and Neural Crest Derivatives-Expressed Transcript 2 |
FOXO1 | Fork head Box O1 |
BMP2 | Bone Morphogenetic Protein 2 a member of the Transforming Growth Factor-Beta (TGF-β) superfamily |
HOXA10 | Homeobox A10 |
IGFBP1 | Insulin-Like Growth Factor-Binding Protein 1 |
PRL | Prolactin |
ZBTB16 | Zinc Finger and BTB Domain-Containing 16 |
PAQR | Progestin and AdipoQ Receptors |
Org OD 02-0 | 10-Ethenyl-19-norprogesterone |
cAMP | Cyclic Adenosine Monophosphate |
Appendix A
References
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Clinical Characteristics | Control (n = 20) | Women with Endometriosis (n = 20) |
---|---|---|
Age years (mean ± SD) | 38.4 (± 6.6) | 33.22 (± 9.7) |
Number of women with full-term pregnancies | 20 | 4 |
Number of women with abortions | 2 | 11 |
Number of women in the proliferative phase during biopsy collection | 13 | 11 |
Number of women in the secretory phase during biopsy collection | 7 | 9 |
GENE | FORWARD 5′3′ | REVERSE 5′3′ |
---|---|---|
HAND 2 | GAGGAAGAAGGAGCTGAACGAA | GTCCGGCCTTTGGTTTTCTTG |
IGFBP1 | TCCTTTGGGACGCCATCAGTAC | GATGTCTCCTGTGCCTTGGCTA |
PRL | CATATTGCGATCCTGGAATGAGC | TCCTCAATCTCTACAGCTTTGGA |
FOXO-1 | AGGDTTAGTGAGCAGGTTACAC | TGGACTGCTTCTCTCAGTTCC |
ZBTB16 | TTGTCTGTGATCAGTGCGGT | GCCATGTCAGTGCCAGTATG |
PAQR8 | AGGACGCAGCAAACAGGACA | GCC AACACAGGCAGGAATAA |
GAPDH | GACAGTCAGCCGCATCTTCT | GCCCAATACGAC CAAATCCGT |
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Velázquez-Hernández, D.M.; Vázquez-Martínez, E.R.; Cruz-Orozco, O.; Silvestri-Tomassoni, J.R.; Sánchez-Ramírez, B.; Olguín-Ortega, A.; Escobar-Ponce, L.F.; Rodríguez-Dorantes, M.; Camacho-Arroyo, I. Membrane Progesterone Receptor Beta Regulates the Decidualization of Endometrial Stromal Cells in Women with Endometriosis. Int. J. Mol. Sci. 2025, 26, 7297. https://doi.org/10.3390/ijms26157297
Velázquez-Hernández DM, Vázquez-Martínez ER, Cruz-Orozco O, Silvestri-Tomassoni JR, Sánchez-Ramírez B, Olguín-Ortega A, Escobar-Ponce LF, Rodríguez-Dorantes M, Camacho-Arroyo I. Membrane Progesterone Receptor Beta Regulates the Decidualization of Endometrial Stromal Cells in Women with Endometriosis. International Journal of Molecular Sciences. 2025; 26(15):7297. https://doi.org/10.3390/ijms26157297
Chicago/Turabian StyleVelázquez-Hernández, Dora Maria, Edgar Ricardo Vázquez-Martínez, Oliver Cruz-Orozco, José Roberto Silvestri-Tomassoni, Brenda Sánchez-Ramírez, Andrea Olguín-Ortega, Luis F. Escobar-Ponce, Mauricio Rodríguez-Dorantes, and Ignacio Camacho-Arroyo. 2025. "Membrane Progesterone Receptor Beta Regulates the Decidualization of Endometrial Stromal Cells in Women with Endometriosis" International Journal of Molecular Sciences 26, no. 15: 7297. https://doi.org/10.3390/ijms26157297
APA StyleVelázquez-Hernández, D. M., Vázquez-Martínez, E. R., Cruz-Orozco, O., Silvestri-Tomassoni, J. R., Sánchez-Ramírez, B., Olguín-Ortega, A., Escobar-Ponce, L. F., Rodríguez-Dorantes, M., & Camacho-Arroyo, I. (2025). Membrane Progesterone Receptor Beta Regulates the Decidualization of Endometrial Stromal Cells in Women with Endometriosis. International Journal of Molecular Sciences, 26(15), 7297. https://doi.org/10.3390/ijms26157297