Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways
Abstract
1. Introduction
2. Results
2.1. Impact of ITN on Inflammatory Responses in ALI Mice
2.2. Effects of ITN on Lung Tissue MDA, SOD, and GSH in ALI Mice
2.3. Cell Viability
2.4. Effects of ITN on PGE2, IL-1β, IL-6, and TNF-α Levels in A549 Cells
2.5. Effects of ITN on Oxidative Stress in A549 Cells
2.6. Effects of ITN on Keap1-Nrf2/HO-1 Pathway in LPS-Induced A549 Cells
2.7. Effects of ITN on MAPK/NF-κB Pathway in LPS-Induced A549 Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Plant Materials and Preparation of NCTP
4.3. Animal Experiment
4.3.1. Model Design
4.3.2. Lung Wet-to-Dry Ratio (W/D Ratio)
4.3.3. Cell Counts and Protein Contents of Bronchoalveolar Lavage Fluid (BALF) in Mice
4.3.4. Lung Tissue Pro-Inflammatory Cytokines and Oxidative Stress Indicators in Mice
4.3.5. Histopathological Analysis
4.4. Cell Experiments
4.4.1. Cell Culture and Treatment
4.4.2. CCK-8 Assay
4.4.3. Pro-Inflammatory Cytokines and Oxidative Stress Indicators in A549 Cells
4.4.4. Western Blot Analysis
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
ITN | isostrictiniin |
NCTP | polyphenol-enriched fraction from Nymphaea candida |
ALI | acute lung injury |
LPS | lipopolysaccharide |
DXM | dexamethasone |
BALF | bronchoalveolar lavage fluid |
Neu | neutrophil |
Lym | lymphocyte |
WBC | while blood cell |
MPO | myeloperoxidase |
IL-1β | interleukin-1β |
IL-6 | interleukin-6 |
TNF-α | tumor necrosis factor-α |
NO | nitric oxide |
PGE2 | prostaglandin-2 |
ROS | reactive oxygen species |
MDA | malondialdehyde |
SOD | superoxide dismutase |
GSH | glutathione |
GSSG | oxidized glutathione |
ELISA | enzyme-linked immunosorbent assay |
MAPK | mitogen-activated protein kinase |
JNK | c-Jun N-terminal kinase |
ERK | extracellular regulated protein kinases |
NF-κB | nuclear factor kappa |
IκBα | inhibitor of NF-κB |
Keap1 | kelch-like ECH-associated protein 1 |
HO-1 | heme oxygenase-1 |
SEM | standard error of mean |
ANOVA | analysis of variance |
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Group | Grades | Analysis | |||
---|---|---|---|---|---|
- | + | ++ | +++ | ||
Control | 5 | 1 | 0 | 0 | 0.1667 ± 0.167 |
Model | 0 | 0 | 4 | 2 | 2.3333 ± 0.211 ## |
DXM, 3 mg/kg | 0 | 5 | 1 | 0 | 1.1667 ± 0.167 ** |
ITN, 25 mg/kg | 0 | 4 | 2 | 0 | 1.3333 ± 0.211 ** |
ITN, 50 mg/kg | 0 | 4 | 2 | 0 | 1.3333 ± 0.211 ** |
ITN, 100 mg/kg | 0 | 5 | 1 | 0 | 1.1667 ± 0.167 ** |
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Ding, W.; Sun, Y.; Tuohudaali, W.; Li, C.; Yao, Y.; Zhao, J. Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways. Int. J. Mol. Sci. 2025, 26, 5912. https://doi.org/10.3390/ijms26125912
Ding W, Sun Y, Tuohudaali W, Li C, Yao Y, Zhao J. Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways. International Journal of Molecular Sciences. 2025; 26(12):5912. https://doi.org/10.3390/ijms26125912
Chicago/Turabian StyleDing, Wanting, Yuan Sun, Wulipan Tuohudaali, Chenyang Li, Yuhan Yao, and Jun Zhao. 2025. "Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways" International Journal of Molecular Sciences 26, no. 12: 5912. https://doi.org/10.3390/ijms26125912
APA StyleDing, W., Sun, Y., Tuohudaali, W., Li, C., Yao, Y., & Zhao, J. (2025). Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways. International Journal of Molecular Sciences, 26(12), 5912. https://doi.org/10.3390/ijms26125912