Genetic Factors Linking Nucleolar Stress with R2 Retrotransposon Expression in Drosophila melanogaster
Abstract
1. Introduction
2. Results
2.1. R2 Expression in Wild Type Ovaries—A Complex Negative Control
2.2. Udd Mutations Affecting R2 Expression—A Clear Positive Control
2.3. R2 Expression in Tissues Depleted for Nopp140
2.4. Over-Expression of Nopp140-RGG but Not Nopp140-True Induces R2 Expression
2.5. Testing R2 Expression in Minute Genetic Backgrounds
3. Discussion
3.1. An Overview
3.2. R2 Expression in Wild Type Ovaries
3.3. Does a Compensation Mechanism Exist to Activate R2-Inserted rDNA Genes?
3.4. Developing a Hypothesis for R2 Induction
4. Materials and Methods
4.1. Fly Stocks
4.2. HCRTM RNA-FISH Hybridization
4.3. BrUTP Labeling
4.4. RT-qPCR
4.5. Microscopy and Ribosome Counts
4.6. Western Blotting
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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X Chromosome Pairs | a | b | c | d | e | f | g | h | i | j | k |
---|---|---|---|---|---|---|---|---|---|---|---|
a | w1118 | ||||||||||
b | RpS3/+ | RpS3/TM2 | |||||||||
c | TM2/+ | ||||||||||
d | RpL38/+ | RpL38/CyO | |||||||||
e | |||||||||||
f | udd0683-G4/+ | udd0683-G4/udd0683-G4 | udd0683-G4/CyO | udd0683-G4/uddNull | udd0683-G4/CyO-GFP | ||||||
g | CyO/+ | ||||||||||
h | uddNull/+ | uddNull/CyO | uddNull/CyO-GFP | ||||||||
i | CyO-GFP/+ | RpL14/TM2 | |||||||||
j | KO121/+ | KO121/TM3-GFP |
Gene | Primer Sequences (5′-3′) | Product Length (bps) |
---|---|---|
GAPDH-Forward | AATTCCGATCTTCGACATGG | 91 |
GAPDH-Reverse | GAAAAAGCGGCAGTCGTAAT | |
Tubulin-Forward | TGTCGCGTGTGAAACACTTC | 96 |
Tubulin-Reverse | AGCAGGCGTTTCCAATCTG | |
Nopp140-Exon 1-Forward | TTTTGTGGGACAAGCGGCTA | 154 |
Nopp140-Exon 1-Reverse | TCTGCTGGAAAACCTTGGCT | |
R2-Forward | AATAGATACGACAGACCGGACATAC | 186 |
R2-Reverse | CATAACCTGGACGCTAATGATATGAC |
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Pandey, S.; Nguyen, A.T.; Maricle, A.K.; DiMario, P.J. Genetic Factors Linking Nucleolar Stress with R2 Retrotransposon Expression in Drosophila melanogaster. Int. J. Mol. Sci. 2025, 26, 5480. https://doi.org/10.3390/ijms26125480
Pandey S, Nguyen AT, Maricle AK, DiMario PJ. Genetic Factors Linking Nucleolar Stress with R2 Retrotransposon Expression in Drosophila melanogaster. International Journal of Molecular Sciences. 2025; 26(12):5480. https://doi.org/10.3390/ijms26125480
Chicago/Turabian StylePandey, Shova, An Tri Nguyen, Audrey K. Maricle, and Patrick J. DiMario. 2025. "Genetic Factors Linking Nucleolar Stress with R2 Retrotransposon Expression in Drosophila melanogaster" International Journal of Molecular Sciences 26, no. 12: 5480. https://doi.org/10.3390/ijms26125480
APA StylePandey, S., Nguyen, A. T., Maricle, A. K., & DiMario, P. J. (2025). Genetic Factors Linking Nucleolar Stress with R2 Retrotransposon Expression in Drosophila melanogaster. International Journal of Molecular Sciences, 26(12), 5480. https://doi.org/10.3390/ijms26125480