Correction: Sturm et al. Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine. Int. J. Mol. Sci. 2024, 25, 4868

Figure Legend

• Figure 2. Impact of blue light exposure on viability of urothelial carcinoma cells. Urothelial carcinoma cell line cultures (BFTC-905; RT-112; SW-1710) were irradiated with blue light (l = 453 nm) at light doses indicated (50, 80,110, 140, 200, 300 J/cm²). Twenty-four hours after light exposure, the number of living cells was detected by MTT assay. Bars represent the mean ± S.D, normalised to the value of the non-treated controls of four individual experiments (n = 4). Statistical evaluation (paired t-test) was carried out with the values of the original measurements, i.e., before the normalisation process to the respective control value shown here (contr.). *, p < 0.05 compared with the respective non-irradiated cell cultures.
• Figure 3. Impact of blue light-activated riboflavin on viability of exposed urothelial carcinoma cell cultures. Urothelial carcinoma cell line cultures (A) BFTC-905; (B) RT-112; (C) SW-1710 were exposed to blue light (grey bars, 110 J/cm²) in the absence (0 µM) or presence of 5, 10, 20 or 25 µM riboflavin. White bars represent the values of the respective non-irradiated control cultures. Twenty-four hours after light exposure, the number of living cells was detected by MTT assay. Bars represent the mean ± S.D, normalised to the value of the non-treated controls of eight individual experiments (n = 8). The statistical evaluation (two-way ANOVA) was carried out with the values of the original measurements, i.e., before the normalisation process to the respective control value shown here (contr.). *, p < 0.05 compared with the value of the respective non-irradiated control cultures.
• Figure 4. Impact of blue light-activated riboflavin on the cytotoxic capacity of gemcitabine. Urothelial carcinoma cell line cultures ((A) BFTC-905; (B) RT-112; (C) SW-1710) were maintained in the absence or presence of 10 or 25 ng/mL gemcitabine (contr., white bars). Additionally, these cultures were incubated with 10 µM riboflavin (10 µM RF, black bars). Finally, control cultures (contr., white bars) were exposed to blue light with a dose of 110 J/cm² (+453 nm, grey bars,) and the riboflavin-containing cultures (10 µM RF, black bars) also were exposed to blue light with a dose of 110 J/cm² (10 µM RF + 453 nm, lined bars). Twenty-four hours after light exposure, the number of living cells was detected by MTT assay. Bars represent the mean ± S.D, normalised to the value of the non-treated controls, of seven individual experiments (n = 7). The statistical evaluation (two-way ANOVA) was carried out with the values of the original measurements, i.e., before the normalisation process to the respective control value shown here (contr.). *, p < 0.05 compared with the respective cultures irradiated in the absence of riboflavin (grey bars); #, p < 0.05 compared with the respective control cultures (contr., white bars); §, p < 0.05 compared with the respective non-irradiated riboflavin-containing cultures (10 µM RF, black bars); $, p < 0.05 compared with the respective control cultures maintained in the absence of gemcitabine (white bars, 0 ng/mL gemcitabine).
• Figure 5. Impact of blue light-activated riboflavin on lipid peroxidation and mitochondrial respiratory chain activity of urothelial carcinoma cell cultures. (A), Urothelial carcinoma cells (BFTC-905) were maintained in the absence or presence of 10, 25 or 50 ng/mL gemcitabine (contr., white bars). Additionally, these cultures were incubated with 10 µM riboflavin (10 µM RF, black bars). Finally, control cultures (contr., white bars) were exposed to blue light with a dose of 110 J/cm² (+453 nm, grey bars,) and the riboflavin-containing cultures (10 µM RF, black bars) also were exposed to blue light with a dose of 110 J/cm² (10 µM RF + 453 nm, lined bars). Twenty-four hours after light exposure, the concentration of malondialdehyde as a lipid peroxidation marker was quantified using a specific assay. Bars represent the mean ± S.D, normalised to the value of the non-treated controls, of three individual experiments (n = 3). The statistical evaluation (paired t-test) was carried out with the values of the original measurements, i.e., before the normalisation process to the respective control value shown here (contr.). *, p < 0.05 compared with all other cultures; #, p < 0.05 compared with the respective control cultures (contr., white bars). Additionally, key parameters of the respiratory chain, basal respiration (B), ATP production rate (C), maximal respiration (D) and relative spare respiration capacity (E) of non-irradiated (white bars) as well as blue light exposed (BL, 110 J/cm²) BFTC-905, RT-112 or SW-1710 tumour cell cultures were detected. The statistical evaluation was carried out with the values of the original measurements. *, p < 0.05 compared with the respective values obtained with the non-irradiated cultures.

Text Correction

Reference

1. Sturm, S.; Niegisch, G.; Windolf, J.; Suschek, C.V. Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine. Int. J. Mol. Sci. 2024, 25, 4868. [Google Scholar] [CrossRef] [PubMed]