Next Article in Journal
Functionalization of Ceramic Scaffolds with Exosomes from Bone Marrow Mesenchymal Stromal Cells for Bone Tissue Engineering
Previous Article in Journal
Applicability of a Chemiluminescence Immunoassay to Screen Postmortem Bile Specimens and Its Agreement with Confirmation Analysis
Previous Article in Special Issue
Molecular Insights into the Synergistic Effects of Putrescine and Ammonium on Dinoflagellates
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 25
Issue 7
10.3390/ijms25073827
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002

by
Marine Vincent
1,†,
Victoire Blanc-Garin
1,†,
Célia Chenebault
1,
Mattia Cirimele
1,2,
Sandrine Farci
1,
Luis Fernando Garcia-Alles
3,
Corinne Cassier-Chauvat
1 and
Franck Chauvat
1,*
1
Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, 91198 Gif-sur-Yvette, France
2
Université Paris-Saclay, ENS Paris-Saclay, 91190 Gif-sur-Yvette, France
3
Toulouse Biotechnology Institute, Université de Toulouse, CNRS, INRAE, INSA, 135 Avenue de Rangueil, 31077 Toulouse, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(7), 3827; https://doi.org/10.3390/ijms25073827
Submission received: 12 January 2024 / Revised: 4 March 2024 / Accepted: 27 March 2024 / Published: 29 March 2024
(This article belongs to the Special Issue Advances in Research of Algae, Cyanobacteria, and Phytoplankton)
Browse Figures
Versions Notes

Abstract

:
Terpenes are high-value chemicals which can be produced by engineered cyanobacteria from sustainable resources, solar energy, water and CO2. We previously reported that the euryhaline unicellular cyanobacteria Synechocystis sp. PCC 6803 (S.6803) and Synechococcus sp. PCC 7002 (S.7002) produce farnesene and limonene, respectively, more efficiently than other terpenes. In the present study, we attempted to enhance farnesene production in S.6803 and limonene production in S.7002. Practically, we tested the influence of key cyanobacterial enzymes acting in carbon fixation (RubisCO, PRK, CcmK3 and CcmK4), utilization (CrtE, CrtR and CruF) and storage (PhaA and PhaB) on terpene production in S.6803, and we compared some of the findings with the data obtained in S.7002. We report that the overproduction of RubisCO from S.7002 and PRK from Cyanothece sp. PCC 7425 increased farnesene production in S.6803, but not limonene production in S.7002. The overexpression of the crtE genes (synthesis of terpene precursors) from S.6803 or S.7002 did not increase farnesene production in S.6803. In contrast, the overexpression of the crtE gene from S.6803, but not S.7002, increased farnesene production in S.7002, emphasizing the physiological difference between these two model cyanobacteria. Furthermore, the deletion of the crtR and cruF genes (carotenoid synthesis) and phaAB genes (carbon storage) did not increase the production of farnesene in S.6803. Finally, as a containment strategy of genetically modified strains of S.6803, we report that the deletion of the ccmK3K4 genes (carboxysome for CO2 fixation) did not affect the production of limonene, but decreased the production of farnesene in S.6803.

1. Introduction

Terpenes constitute a large family of chemicals often produced by plants (attraction of pollinators and/or protection against pathogens) in low quantities and at high prices. They are used for the production of fragrances, pharmaceuticals, pesticides, solvents and potentially biofuels [1,2,3]. All terpenes (general formula (C5H8)n) derive from the five-carbon (C5) building blocks dimethylallyl pyrophosphate (DMAPP) and isopentenyl pyrophosphate (IPP). The head-to-tail covalent linkage of DMAPP and IPP catalyzed by the geranyl diphosphate synthase (GPPS) enzyme generates the geranyl diphosphate (GPP) precursor of monoterpenes (C10H16), such as limonene. Then, the addition of another IPP unit on GPP catalyzed by the farnesyl diphosphate synthase (FPPS) forms the farnesyl pyrophosphate (FPP) precursor of sesquiterpenes (C15H24), such as farnesene (Figure 1).
In addition to plants, which are better used for food, cyanobacteria, the robust photosynthetic prokaryotes colonizing our planet, can be used for the ecologically responsible production of terpenes from sunlight and CO2 (for reviews see [1,2,3]). Cyanobacteria fix CO2 using the Calvin–Benson–Bassham (CBB) pathway in which the key enzyme ribulose bisphosphate carboxylase/oxygenase (RubisCO) catalyzes the addition of one molecule of CO2 to one molecule of ribulose biphosphate produced by the other key enzyme phosphoribulokinase (PRK). In parallel, they have developed the carboxysome subcellular compartment (assembled from various shell proteins such as CcmK3 and CcmK4) to encapsulate RubisCO in a CO2-rich environment that favors its carbon-fixing (carboxylase) activity over its detrimental oxygenase activity (for reviews see [4,5]). A part of the photosynthetically fixed carbon is used by the methylerythritol-4-phosphate (MEP) pathway to produce DMAPP and IPP (Figure 1), which are transformed by a geranylgeranyl pyrophosphate synthase (CrtE) into GPP, FPP and GGPP, which is used by the carotene hydroxylases (CrtR and CruF) to produce chlorophyll and carotenoids [2,3,6]. GPP and FPP can also be transformed into terpenes, following introduction and expression in cyanobacteria of synthetic terpene-synthase encoding genes adapted to the cyanobacterial codon usage [2,3]. The unicellular euryhaline cyanobacteria Synechocystis sp. PCC 6803 (hereafter S.6803) and Synechococcus sp. PCC 7002 (S.7002, coastal organism) are interesting for this purpose. They have good genetics (one can easily delete or overexpress genes of interests) and they grow well in sea and brackish waters to preserve freshwater resources for agriculture [7].
In the frame of our exploration of the ability of physiologically different cyanobacteria for the photoproduction of chemically different terpenes, we previously codon-adapted several genes encoding terpene synthases, in an RSF1010-derived replicative plasmid or in a neutral chromosome site [8,9,10,11]. The results showed that S.6803 produces farnesene better than bisabolene, limonene, santalene and pinene, whereas S.7002 produces limonene more efficiently than bisabolene and pinene [9]. In the present study, we tested and compared several genetic strategies to address the improvement of farnesene production in S.6803 and limonene production in S.7002 [11].

2. Results and Discussion

2.1. The Overexpression of the RubisCO Genes from Synechococcus PCC 7002 Increases Farnesene Production in Synechocystis PCC 6803

Previous studies showed that overexpressing the RubisCO-encoding genes can improve cell growth and/or the photosynthetic production of high value chemicals in S.6803 and/or S.7002 [12,13,14,15]. In this study, we attempted to improve farnesene production in S.6803 by introducing an extra copy of the RubisCO-encoding genes in the S.6803-ChrFS expressing the Picea abies α-farnesene synthase gene from a neutral site of its chromosome [9]. To increase the odds of improving RubisCO activity in S.6803, we used extra RubisCO genes from not only S.6803, but also physiologically diverse cyanobacteria studied in our laboratory. These are Cyanothece sp. PCC 7425 (hereafter C.7425) and S.7002 where their RubisCO genes constitute the rbcLXS operon encoding the large (RbcL) and small (RbcS) RubisCO subunits, and the RbcX chaperon (See Cyanobase). We also used the rbcLS operon of Synechococcus elongatus PCC 7942 (S.7942) where rbcX is located away from rbcLS [16,17].
These RubisCO operons were PCR amplified from their respective cyanobacterial genomes (Figure S1) using oligonucleotides (Table S1) that introduced convenient restriction site upstream of the ATG start codon of rbcL (NdeI for S.6803, S.7002 and S.7942, and XhoI for C.7425) and downstream of the TAA stop codon of rbcS (EcoRI for S.6803, PvuII for both S.7002 and S.7942, and BspEI for C.7425). After restriction, the RubisCO operons were cloned downstream of the strong pR promoter of the RSF1010-derived replicative pC plasmid vector [9] opened with the same enzymes. The resulting SmR/SpR plasmids, pCrbc6803, pCrbc7002, pCrbc7942 and pCrb7425 (Figure S1 and Table S2) and the (empty) pC control vector were introduced by conjugation in the S.6803-ChrFS engineered strain that produces farnesene [9]. In each case, two independent SmR/SpR clones were selected and analyzed by PCR and DNA sequencing (Figure S1). The data showed that all these pC-derived plasmids, pCrbC7425, pCrbc7942, pCrbc6803 and pCrbc7002, replicate stably in S.6803 with no impact on cell growth (Figure 2), similar to the pC control vector 7002 [11]. The resulting recombinant strains, which also produce RubisCO from their indigenous chromosomal RubisCO genes, are represented in Figure S2.
The production of farnesene by the S.6803-chrFS strains propagating the RubisCO-producing plasmids pCrbc6803, pCrbc7002, pCrbc7425 and pCrbc7942 was measured over 21-day periods of photoautotrophic growth, as previously described [9]. GC–MS analysis of the terpene-trapping dodecane overlay samples from all tested strains showed a peak with similar retention time and ion chromatogram as a pure standard of α-farnesene. As compared to the S.6803-chrFS control strain, which has a natural level of RubisCO, the level of farnesene production driven by the pCrbc7002 plasmid showed that high-level expression of the RubisCO genes from S.7002 enhanced farnesene production (about 2-fold at day 21, Figure 2). Unlike pCrbc7002, the pCrbc6803, pCrbc7425 and pCrbc7942 plasmids did not increase farnesene production, though they express their RubisCO genes from the same strong promoter and ribosome binding site as pCrbc7002. Together, these findings suggest that the RubisCO of S.7002 is more active in S.6803 than the other tested RubisCO, including the endogenous enzyme of S.6803, which cannot be degraded by a possible protease recognizing foreign proteins.

2.2. The Overexpression of the Phosphoribulokinase Gene from Cyanothece PCC 7425 Increases Farnesene Production in Synechocystis PCC 6803

As the elimination of the CP12 negative regulator of the phosphoribulokinase (PRK) enzyme was shown to improve terpene production in S.6803 [10], we decided to overexpress the prk gene as an attempt to enhance farnesene production. To increase the odds of enhancing PRK activity, we decided to clone in the pC plasmid vector an extra copy of prk from not only S.6803, but also C.7425, S.7002 and S.7942. These prk genes were PCR amplified from their respective cyanobacterial genome using oligonucleotide primers (Table S1) that embedded their ATG start codon in a NdeI restriction site (CATATG), and introduced an EcoRI site downstream of their stop codons: TAA (S.6803) or TAG (S.7002, S.7942 and C.7425). After restriction with both NdeI and EcoRI, these genes were cloned in pC opened with the same enzymes, and transformed to E. coli. All attempts to clone the prk genes from S.6803, S.7002 and S.7942 in pC were unsuccessful, indicating that cyanobacterial PRKs are toxic to E. coli. Supporting this interpretation, previous cloning in E.coli of the prk gene from S.7942 [18] and S.6803 [19], for in vitro analysis of PRK, employed an inducible promoter system that allowed for the decoupling of E.coli growth from the production of PRK.
Nevertheless, we were able to clone the prk gene from C.7425 (prk7425) downstream of the strong constitutive pR promoter of the pC plasmid (Table S2), suggesting that PRK7425 is somehow less active in E. coli than the other cyanobacterial PRKs presently tested. The resulting plasmid, pCprk7425 (Table S2), was introduced by conjugation in the S.6803-chrFS strain expressing the farnesene synthase gene from its chromosome [9]. A SmR/SpR clone (Figure S2) was analyzed. It showed that pCprk7425 does not alter the growth of S.6803 (Figure 3). The production of farnesene during the photoautotrophic growth of the S.6803-chrFS-pCprk7425 reporter strain (Figure S2) was analyzed for 21 days and compared to that of the S.6803-chrFS control strain. The data showed that increasing the expression of prk enhances (about three-fold) the production of farnesene in S.6803 (Figure 3). This result is consistent with both our previous report on the CP12-deleted mutant [10] and the above finding that overexpressing RubisCO genes improved farnesene production in S.6803 (Figure 2). Collectively, these data indicate that increasing CO2 fixation enhances terpene production in S.6803.

2.3. The Overexpression of the Genes Encoding RubisCO and PRK Enzymes Does Not Increase Limonene Production in Synechococcus PCC 7002

We also attempted to improve limonene production in the previously described S.7002-chrLS strain that carries the Mentha spicata 4S-limonene synthase gene in a neutral chromosome site [11]. Therefore, the above-described plasmids overexpressing the studied phosphoribulokinase gene (pCprk7425) or RubisCO operons (pCrbc6803, pCrbc7002, pCrbC7425 and pCrbc7942) were introduced by conjugation in S.7002-chrLS. The growth and limonene production of the resulting reporter strains (Figure S3) appeared to be similar to that of the S.7002-chrLS control strain (Figure 4).
Collectively, the present findings show that the photoautotrophic growth of both S.6803 and S.7002 is not limited by the natural abundance of their indigenous enzymes RubisCO and PRK. In contrast, the production of terpenes is limited by the natural abundance of both RubisCO and PRK in S.6803 (Figure 2 and Figure 3), but not in S.7002 (Figure 4). These differences remind us that a model cyanobacterium is essentially a model of itself. Consequently, it is therefore important to study several cyanobacteria in parallel (in the same laboratory) to better understand their common and specific properties, and exploit the resulting knowledge for biotechnological purposes.

2.4. The Overexpression of the crtE Genes from Synechocystis PCC 6803 or Synechococcus PCC 7002 Decreases the Production of Both Farnesene and Limonene in Synechocystis PCC 6803

One of the challenges in using cyanobacteria for terpene production is the competition for prenyl pyrophosphates (GPP, FPP and GGPP) between the synthesis of photosynthetic pigments and the intended terp [20] ene (Figure 1). As most cyanobacteria, such as S.6803 and S.7002, use a single GGPP synthase (named CrtE) for the sequential synthesis of GPP, FPP and GGPP [6,21,22,23], several authors overexpressed synthetic genes encoding FPPS (IspA) from E. coli [24,25,26] or GPPS from plants [27,28,29,30] to improve (2–3 fold) terpene production in S.6803, S.7942 (S.7002 was not tested) or Synechococcus elongatus UTEX 2973. These moderate improvements could result from these heterologous enzymes having a low activity and/or stability in cyanobacteria. This eventuality prompted us to test the influence on terpene production of the CrtE enzymes from both S.6803 and S.7002, which have structural differences [6,23]. Therefore, the protein coding sequences of the crtES.6803 and crtES.7002 genes were PCR amplified from the genomes of S.6803 or S.7002 using specific oligonucleotides (Table S1) which flanked them with a unique restriction site upstream of their start codon, and another unique restriction site behind their stop codon (Table S2). After restriction, these DNA fragments were cloned downstream the Picea abies α-farnesene synthase gene (FS) or Mentha spicata 4S-limonene synthase gene (LS) of our previously described pCFS and pCLS plasmids [8,9] opened with the same enzymes (XhoI and EcoRI for pCFS, and EcoRI and AclI for pCLS). The resulting plasmids (Table S2) pCFScrtE6803 and pCFScrtE7002 (expression of the FScrtE6803 and FScrtE7002 operons, respectively), and pCLScrtE6803 and pCLScrtE7002 (expression of the LScrtE6803 or LScrtE7002 operons) were introduced by conjugation in S.6803 (Figure S4), where they appeared to replicate stably (Figure S5). The resulting S.6803 reporter strains (Figure S4), which also expressed their indigenous crtE gene from their chromosome, grew similarly to the control strains propagating the pCFS or pCLS control plasmids (Figure 5).
The production of farnesene directed by the pCFScrtE6803 and pCFScrtE7002 plasmids was lower (about three-fold) than the level driven by the pCFS control plasmid (Figure 5). Similarly, the production of limonene directed by the pCLScrtE6803 and pCLScrtE7002 plasmids were smaller (about six-fold and three-fold, respectively) than the level driven by the pCLS control plasmid (Figure 5). Collectively, these data indicate that the natural abundance of the CrtE enzyme is not limiting terpene production in S.6803. The present finding that an increased level of CrtE actually decreases terpene production suggests that the additional molecules of the GGPP metabolite synthesized by the extra molecules of CrtE might inhibit the MEP pathway and/or the studied terpene synthases, similarly to the feedback inhibition caused by the accumulation of IPP [3]. In addition, the increased level of CrtE might negatively influence the hypothetical metabolic pairing of CrtE with the GGPP phosphatase [23], which could somehow act in terpene synthesis.

2.5. The Overexpression of the crtE Gene from Synechocystis PCC 6803, but Not Synechococcus PCC 7002, Increases Farnesene Production in Synechococcus PCC 7002

The influence of an extra copy of the crtE gene on terpene production in S.7002 was tested as follows. The above-described pCFScrtE6803 and pCFScrtE7002 plasmids overexpressing the operonic genes encoding the farnesene synthase and the CrtE enzyme from either S.6803 or S.7002 were introduced by conjugation in S.7002, along with the pCFS control plasmid expressing only the farnesene synthase gene (Figure S6). All three plasmids appeared to replicate stably (Figure S7) without affecting cell growth (Figure 5). As compared to pCFS, pCFScrtE6803 and pCFScrtE7002, respectively, increased and (slightly) decreased farnesene production in S.7002 (Figure 6). These data suggest that the activity of the CrtE6803 and CrtE7002 enzymes might be different in S.7002.

2.6. The Deletion of PHB Synthesis Genes Does Not Increase Terpene Production in Synechocystis PCC 6803.

In S.6803, most of the photosynthetically fixed carbon is used for the production of biomass and abundant carbon stores such as glycogen and polyhydroxybutyrates (PHB) [31,32], while only 5% of the carbon is allocated to the MEP pathway for the synthesis of photosynthetic pigments (carotenoids, chlorophyll and quinone) [2,33]. As an attempt to redirect the carbon flux toward the MEP pathway to enhance terpene production in S.6803, we deleted the two adjacent genes phaAB encoding the acetoacetyl-CoA thiolase (PhaA) and acetoacetyl-CoA reductase (PhaB) enzymes, which catalyze the first step in PHB synthesis [34,35,36]. Therefore, a ΔphaAB::KmR DNA cassette (Table S2) harboring a transcription-terminator less KmR marker in place of most of the phaA and phaB genes (from codon 93 of phaA to codon 141 of phaB) was constructed, as follows (Figure S8). The two 300 bp chromosomal DNA regions flanking the phaAB DNA region to be deleted were synthesized by TWIST Bioscience as a single DNA segment harboring an EcoRV restriction site in its middle where we cloned the KmR marker (a HincII segment from pUC4K) in the same orientation as the phaAB genes that it replaced (Figure S8). The ΔphaAB::KmR DNA cassette was verified by PCR and DNA sequencing. It was then transformed to S.6803, where homologous DNA recombination integrated the KmR marker in place of the phaAB gene, in all copies of the S.6803 chromosome (Figure S8). Then, the SmR/SpR plasmids pCFS and pCLS [8,9] were introduced by conjugation in the ΔphaAB::KmR mutant (Figure S8 and (Figure S9). In each case, several SmR/SpR/KmR clones were studied. The data showed that the deletion of the phaAB genes did not increase the photoproduction of farnesene or limonene in S.6803 (Figure 7).
This approach of deleting PHB synthesis genes to redirect photosynthetically fixed carbons toward terpene production could not be tested in S.7002 because this cyanobacterium does not synthesize PHB [37].

2.7. The Deletion of the Carotenoid Synthesis Genes crtR and cruF Decreases the Production of Farnesene in Synechocystis PCC6803

The production of sesquiterpenes, such as farnesene, competes with the synthesis of photosynthetic pigments (chlorophyll and carotenoids) because they use the same precursor metabolite FPP [38] (Figure 1). To save FPP for a better farnesene production, we decided to decrease the consumption of the FPP-derived metabolite GGPP used for carotenoid synthesis (Figure 1). Therefore, we deleted the genes crtR (β-carotene hydroxylase) and cruF (γ-carotene hydroxylase) acting in GGPP-consuming synthesis of carotenoids. For crtR deletion, a ΔcrtR::KmR DNA cassette (Table S2) was constructed by replacing the first 82 codons of crtR by a transcription-terminator-less KmR marker, as follows. The two 300 bp chromosomal DNA regions flanking the first 82 codons of crtR to be deleted were synthesized by TWIST Bioscience as a single DNA segment harboring an EcoRV restriction site in its middle, where we cloned the transcription-terminator-less KmR marker (a HincII segment from the pUC4K plasmid) in the same orientation as crtR (Figure S10). The ΔcrtR::KmR DNA cassette was verified by PCR and DNA sequencing. It was transformed to S.6803, where homologous DNA recombination replaced crtR by the KmR marker in all copies of the S.6803 chromosome (Figure S10). The resulting ΔcrtR::KmR mutant grew as fit as the WT strain under standard light. This finding showed that crtR is not essential for the photoautotrophic growth of S.6803, in agreement with previous reports [39,40]. Then, the SmR/SpR pCFS plasmid was introduced by conjugation in the ΔcrtR::KmR mutant (Figure S10), and the production of farnesene by two SmR/SpR/KmR clones was analyzed during photoautotrophic growth (Figure 8). The data showed that the deletion of crtR does not increase the photoproduction of farnesene in S.6803 (it was decreased), unlike what was expected.
For cruF deletion, a ΔcruF::KmR DNA cassette (Table S2) was constructed (Figure S12) by replacing an internal part of the cruF coding sequence (CS, from codon 100 to codon 271) by the KmR marker. The two 300 bp DNA regions flanking the 171 codons of cruF to be deleted were synthesized by TWIST Bioscience as a single DNA segment harboring an EcoRV site in its middle, where we cloned the KmR marker in the same orientation as cruF. The ΔcruF::KmR DNA cassette was verified by PCR and nucleotide sequencing. It was then transformed to S.6803 where homologous DNA recombination replaced the 171 codons of cruF by the KmR marker in all copies of the S.6803 chromosome (Figure S12). The resulting ΔcruF::KmR mutant grew as fit as the WT strain under standard light showing, for the first time, that cruF is not crucial for the photoautotrophic growth of S.6803.
Then, the SmR/SpR pCFS plasmid was introduced by conjugation in the ΔcruF::KmR mutant (Figures S11 and S12), and the farnesene production of two independent SmR/SpR/KmR clones was analyzed (Figure 8). As reported above in the case of crtR, the deletion of cruF did not increase, but decreased, the photoproduction of farnesene in S.6803 (Figure 8).
Since the strategy of deleting the crtR and cruF genes did not increase the production of farnesene in S.6803, it was not tested in S.7002.

2.8. Deletion of Both the ccmK3 and ccmK4 Genes Encoding Carboxysome Shell Proteins Does Not Alter the Production of Limonene, but Decreases the Production of Farnesene in Synechocystis PCC 6803

In addition to the engineering of genetically modified cyanobacterial organisms (GMOs) for the photosynthetic production of high-value chemicals, it is important to consider strategies to limit accidental release of these GMOs in natural environments. For this purpose, an interesting target is the carbon concentrating mechanism (CCM) that cyanobacteria use to grow in the low CO2 concentration of their natural aquatic biotopes. The CCM system uses the carboxysome subcellular compartment, assembled from various Ccm shell proteins, which encapsulates the RubisCO enzyme in a CO2-rich environment favoring its carbon-fixing (carboxylase) activity over its detrimental oxygenase activity (for reviews see [4,5]). As a biocontainment strategy, previous workers have deleted carboxysome genes of S.7002 and S.7942 GMO to impose a high-CO2 requirement phenotype (HCR) preventing their escape from their cultivation photobioreactors [41,42]. Such a HCR phenotype did not negatively impact L-lactate production in S.7002 [41], but it decreased (about two-fold) farnesene production in S.7942 [42]. Since the HCR containment of GMO has not yet been tested in S.6803, we decided to delete the ccmK3 and ccmK4 adjacent genes encoding the CcmK3 and CcmK4 carboxysome shell proteins in S.6803 [43] and S.7942 [44]. These proteins were shown to be required for cell growth at low CO2 in S.7942 [44,45], and for optimal photoautotrophic growth in S.6803 [46].
A ΔccmK3K4::KmR DNA cassette (Table S2) was constructed to delete both the ccmK3 and ccmK4 colinear genes (from the start of ccmK3 coding sequence (CS) to the stop codon of ccmK4). The 250 bp regions upstream the ccmK3 CS and downstream the ccmK4 CS were synthesized by TWIST Bioscience as a single DNA segment harboring SwaI and a BamHI restriction sites where we cloned the KmR marker (using the same enzymes) in the same orientation as the ccmK3 and ccmK4 CS it replaced (Figure S13). The ΔccmK3K4::KmR grew as healthy as the WT strain in the Na2CO3-rich MM medium, but was unable to grow under atmospheric (low) CO2 levels (MM0 medium), in agreement with previous findings in S.7942 [44,45] and S.6803 [46].
Then, the SmR/SpR plasmids pCFS and pCLS were introduced by conjugation in the ΔccmK3K4::KmR double mutant, and the production of terpenes in the resulting reporter strains (Figures S13 and S14) was analyzed during photoautotrophic growth in the Na2CO3-rich MM medium. The data showed that the deletion of ccmK3K4 did not alter the photoproduction of limonene (monoterpene, C10) in S.6803 (Figure 9), as expected. In contrast, the deletion of ccmK3K4 decreased the production of farnesene (sesquiterpene, C15). Interestingly, these data showed that the more carbon atoms are used to produce the studied terpene, the more the production is decreased by the ΔccmK3K4::KmR deletion (the production of farnesene (C15) is more important than that of limonene (C10)).

3. Materials and Methods

3.1. Bacterial Strains and Growth Conditions

E. coli strains used for DNA manipulations (TOP10 and NEB10 beta, Table S2) or conjugative transfer to S.6803 and S.7002 (CM404, Ref. [9]) of pC-derived replicative plasmids (Table S2) were grown at 37 °C (TOP10 and NEB10 beta) or 30 °C (CM404) on LB medium containing the selective antibiotics: ampicillin (Amp) 100 μg·mL−1, kanamycin (Km) 50 μg·mL−1, streptomycin (Sm) 25 μg·mL−1 or spectinomycin (Sp) 75 μg·mL−1.
S.6803 and S.7002 strains were grown at 30 °C under continuous white light (2500 lux; 31.25 μE·m−2·s−1) and agitation (140 rpm, Infors rotary shaker) in liquid mineral medium, MM, i.e., BG11 [47] enriched with 3.78 mM Na2CO3 for S.6803 [9], or A+ supplemented with B12 vitamin (4 μg·L−1) for S.7002 [48]. The terpene-producing strains were grown in the presence of the selective antibiotics: Km 50 μg·mL−1 for both S.6803 and S.7002, Sm 5 μg·mL−1 and Sp 5 μg·mL−1 for S.6803, and Sm 50 μg·mL−1 and Sp 50 μg·mL−1 for S.7002. Growth was monitored by regular measurements of optical density at 750 nm (OD750) with a spectrophotometer (Jenway 6700).

3.2. Genetic Manipulations and Gene Transfer Techniques

The studied cyanobacterial genes were amplified by polymerase chain reaction (PCR) from cyanobacterial DNA with specific oligonucleotide primers (Table S1) using Hot start Phusion polymerase (ThermoFisher Science France, Illkirch-Graffenstaden, France). PCR products were digested with appropriate restriction enzymes and cloned either in the RSF1010-derived autonomously replicating pC vector for high-level gene expression [9,11], or a commercial E. coli plasmid for gene deletion. All DNA constructions were verified by PCR and DNA sequencing (Mix2Seq Kit, Eurofins Genomics France, Nantes, France) using appropriate oligonucleotide primers (Table S1). The pC-derived plasmids (Table S2) were introduced in S.6803 and S.7002 by conjugation [9,11] using a triparental mating where each cyanobacterial strain was coincubated for 72 h with the E. coli strains CM404 and a TOP10 (or NEB10 beta) strain propagating a pC-derived plasmid. The DNA deletion cassettes were introduced in S.6803 by transformation [10]. Transformants and conjugants were then plated on MM solidified with 1% Bacto Agar (Difco France, Saint-Ferréol, France), which contained Km 50 μg·mL−1 or both Sp 5 μg·mL−1 and Sm 5 μg·mL−1, respectively, and subsequently incubated at 30 °C under standard light for 7 to 10 days. The presence of the gene deletion cassette, or the terpene-synthase-encoding gene propagated in pC-derived plasmids or a neutral chromosomal site was verified by PCR and DNA sequencing (Mix2Seq Kit, Eurofins Genomics) using appropriate oligonucleotide primers (Table S1).

3.3. Terpenes Collection, and Quantification by Gas Chromatography–Mass Spectrometry

S.6803 and S.7002 engineered strains were grown photoautotrophically in the presence of selective antibiotics in 250 mL Erlenmeyer flasks containing 50 mL cell suspensions covered with a nontoxic 20% (v/v) dodecane overlay (analytical grade, Sigma-Aldrich, St. Louis, MO, USA) to trap terpenes [8,9,11,28,49]. At the specified time intervals, 300 μL of these dodecane overlays was collected, and 1 μL of these samples was injected into a GC–MS apparatus (Trace1300 (GC) + ISQ LT (MS), ThermoScientific) equipped with a TG-5MS column (30 m × 0.25 mm × 0.25 µm) and operated with He carrier gas at 1.0 mL·min−1; ionization voltage 70 eV, transfer line temperature 250 °C; ion source temperature 200 °C. Analyses were carried out in the selected ion monitoring mode: m/z = 50–650, as we previously described, using split modes of 10:1 (limonene) or 5:1 (farnesene). Terpenes were quantified as we previously described [8,9,11].

3.4. Statistics

Unless otherwise stated, the data presented represent independent experimental triplicates of the mean and are presented as mean ± standard deviation (SD). The differences between means of the individual groups were analyzed using t-test (p < 0.05, symbolized by *).

4. Conclusions

In this study, we have tested various genetic strategies to attempt to enhance farnesene production in S.6803 and limonene production in S.7002 (Table 1). We report that the overexpression of the genes encoding the key CO2-fixing enzymes RubisCO and phosphoribulokinase increase the production of terpenes in S.6803, but not in S.7002. Furthermore, the overexpression of the crtE gene (synthesis of terpene precursors) from S.6803, but not S.7002, increases farnesene production in S.7002. In contrast, the overexpression of the crtE genes from S.6803 or S.7002 decreases farnesene production in S.6803. Collectively, these results emphasize the physiological differences between S.6803 and S.7002, the two model cyanobacteria that are often used for biotechnology projects [7]. These differences remind us that a model cyanobacterium is essentially a model of itself. Consequently, it is important to study several cyanobacteria in parallel (in the same laboratory) to better understand and compare their common and specific properties, and exploit this knowledge for biotechnological purposes. Here, we also showed that the deletion of the crtR and cruF genes (carotenoid synthesis) and phaAB genes (carbon storage) did not increase the production of farnesene in S.6803. Finally, we have tested a containment strategy for genetically modified organisms (GMOs). We report that the deletion of the ccmK3K4 genes (carboxysome for CO2 fixation) did not impact the production of limonene, but decreased the production of farnesene in S.6803. Collectively, these findings show that the influence on terpene production of the presently tested genetic engineering strategies depends on the nature of the studied cyanobacterial chassis and metabolic enzymes. It is clear from these results and recent data in the literature [20] that one need better understand the metabolism of cyanobacteria to engineer efficient strains for an economically viable photoproduction of terpenes.

Supplementary Materials

The supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms25073827/s1. Ref. [50] is cited in Supplementary Materials.

Author Contributions

Conceptualization, F.C. and C.C.-C.; methodology, F.C. and C.C.-C.; validation, M.V., C.C., V.B.-G., M.C., S.F. and L.F.G.-A.; formal analysis, M.V., C.C., V.B.-G., M.C. and S.F.; investigation, C.C., V.B.-G., M.V., M.C., S.F., C.C.-C. and F.C.; resources, F.C., C.C.-C. and L.F.G.-A.; writing—original draft preparation, F.C. and C.C.-C.; writing—review and editing, F.C., C.C.-C., M.V., C.C., V.B.-G. and L.F.G.-A.; supervision, F.C. and C.C.-C.; project administration, F.C. and C.C.-C.; funding acquisition, F.C., C.C.-C. and L.F.G.-A. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded in part by the CEA program Focus ECC, and the ANR projects CalvinDesign (ANR-17-CE05-001) and SpaceHex SpaceHex (ANR-19-CE09-0032-01).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Truly data are contained within the article and Supplementary Materials.

Acknowledgments

We thank Frédéric Taran for access to GC–MS.

Conflicts of Interest

The authors declare no conflicts of interest.

References

  1. Pattanaik, B.; Lindberg, P. Terpenoids and their biosynthesis in cyanobacteria. Life 2015, 5, 269–293. [Google Scholar] [CrossRef] [PubMed]
  2. Lin, P.C.; Pakrasi, H.B. Engineering cyanobacteria for production of terpenoids. Planta 2019, 249, 145–154. [Google Scholar] [CrossRef] [PubMed]
  3. Rautela, A.; Kumar, S. Engineering plant family TPS into cyanobacterial host for terpenoids production. Plant Cell Rep. 2022, 41, 1791–1803. [Google Scholar] [CrossRef] [PubMed]
  4. Veaudor, T.; Blanc-Garin, V.; Chenebault, C.; Diaz-Santos, E.; Sassi, J.F.; Cassier-Chauvat, C.; Chauvat, F. Recent advances in the photoautotrophic metabolism of cyanobacteria: Biotechnological implications. Life 2020, 10, 71. [Google Scholar] [CrossRef] [PubMed]
  5. Kupriyanova, E.; Pronina, N.A.; Los, D.L. Adapting from Low to High: An Update to CO2-Concentrating Mechanisms of Cyanobacteria and Microalgae. Plants 2023, 12, 1569. [Google Scholar] [CrossRef] [PubMed]
  6. Feng, Y.; Morgan, R.M.L.; Fraser, P.D.; Hellgardt, K.; Nixon, P.J. Crystal Structure of Geranylgeranyl Pyrophosphate Synthase (CrtE) Involved in Cyanobacterial Terpenoid Biosynthesis. Front. Plant Sci. 2020, 11, 505897. [Google Scholar] [CrossRef] [PubMed]
  7. Cassier-Chauvat, C.; Blanc-Garin, V.; Chauvat, F. Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications. Genes 2021, 12, 500. [Google Scholar] [CrossRef]
  8. Chenebault, C.; Diaz-Santos, E.; Kammerscheit, X.; Görgen, S.; Ilioaia, C.; Streckaite, S.; Gall, A.; Robert, B.; Marcon, E.; Buisson, D.-A.; et al. A Genetic Toolbox for the New Model Cyanobacterium Cyanothece PCC 7425: A Case Study for the Photosynthetic Production of Limonene. Front. Microbiol. 2020, 11, 586–601. [Google Scholar] [CrossRef]
  9. Blanc-Garin, V.; Chenebault, C.; Diaz-Santos, E.; Vincent, M.; Sassi, J.F.; Cassier-Chauvat, C.; Chauvat, F. Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes. Biotechnol. Biofuels 2022, 15, 110. [Google Scholar] [CrossRef]
  10. Blanc-Garin, V.; Veaudor, T.; Sétif, P.; Gontero, B.; Lemaire, S.D.; Chauvat, F.; Cassier-Chauvat, C. First in vivo analysis of the regulatory protein CP12 of the model cyanobacterium Synechocystis PCC 6803: Biotechnological implications. Front. Plant Sci. 2022, 13, 999672. [Google Scholar] [CrossRef]
  11. Chenebault, C.; Blanc-Garin, V.; Vincent, M.; Diaz-Santos, E.; Goudet, A.; Cassier-Chauvat, C.; Chauvat, F. Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes. Biomolecules 2023, 13, 504. [Google Scholar] [CrossRef] [PubMed]
  12. Atsumi, S.; Higashide, W.; Liao, J.C. Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nat. Biotechnol. 2009, 27, 1177–1180. [Google Scholar] [CrossRef] [PubMed]
  13. Ruffing, A.M. Improved free fatty acid production in cyanobacteria with Synechococcus sp. PCC 7002 as host. Front. Bioeng. Biotechnol. 2014, 2, 17. [Google Scholar] [CrossRef] [PubMed]
  14. Liang, F.; Englund, E.; Lindberg, P.; Lindblad, P. Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio. Metab. Eng. 2018, 46, 51–59. [Google Scholar] [CrossRef] [PubMed]
  15. Tharasirivat, V.; Jantaro, S. Increased Biomass and Polyhydroxybutyrate Production by Synechocystis sp. PCC 6803 Overexpressing RuBisCO Genes. Int. J. Mol. Sci. 2023, 24, 6415. [Google Scholar] [CrossRef] [PubMed]
  16. Emlyn-Jones, D.; Woodger, F.J.; Price, G.D.; Whitney, S.M. RbcX can function as a Rubisco chaperonin, but is non-essential in Synechococcus PCC7942. Plant Cell Physiol. 2006, 47, 1630–1640. [Google Scholar] [CrossRef] [PubMed]
  17. Bracher, A.; Whitney, S.M.; Hartl, F.U.; Hayer-Hartl, M. Biogenesis and Metabolic Maintenance of Rubisco. Annu. Rev. Plant Biol. 2017, 68, 29–60. [Google Scholar] [CrossRef] [PubMed]
  18. Kobayashi, D.; Tamoi, M.; Iwaki, T.; Shigeoka, S.; Wanado, A. Molecular characterization and redox regulation of phosphoribulokinase from the cyanobacterium Synechococcus sp. PCC 7942. Plant Cell Physiol. 2003, 44, 269–276. [Google Scholar] [CrossRef]
  19. Nishiguchi, H.; Liao, J.; Shimizu, H.; Matsuda, F. Novel allosteric inhibition of phosphoribulokinase identified by ensemble kinetic modeling of Synechocystis sp. PCC 6803 metabolism. Metab. Eng. Commun. 2020, 11, e00153. [Google Scholar] [CrossRef]
  20. Zhou, J.; Xu, S.; Li, H.; Xi, H.; Cheng, W.; Yang, C. A Ribulose-5-phosphate Shunt from the Calvin-Benson Cycle to Methylerythritol Phosphate Pathway for Enhancing Photosynthetic Terpenoid Production. ACS Synth. Biol. 2024, 13, 876–887. [Google Scholar] [CrossRef]
  21. Ohto, C.; Ishida, C.; Nakane, H.; Nishiro, T.; Obata, S. A thermophilic cyanobacterium Synechococcus elongatus has three different Class I prenyltransferase genes. Plant Mol. Biol. 1999, 40, 307–321. [Google Scholar] [CrossRef]
  22. Rodrigues, J.S.; Lindberg, P. Metabolic engineering of Synechocystis sp. PCC 6803 for improved bisabolene production. Metab. Eng. Commun. 2021, 12, e00159. [Google Scholar] [CrossRef]
  23. Satta, A.; Esquirol, L.; Ebert, B.E.; Newman, J.; Peat, T.S.; Plan, M.; Schenk, G.; Vickers, C.E. Molecular characterization of cyanobacterial short-chain prenyltransferases and discovery of a novel GGPP phosphatase. FEBS J. 2022, 289, 6672–6693. [Google Scholar] [CrossRef] [PubMed]
  24. Valsami, E.A.; Psychogyiou, M.E.; Pateraki, A.; Chrysoulaki, E.; Melis, A.; Ghanotakis, D.F. Fusion constructs enhance heterologous β-phellandrene production in Synechocystis sp. PCC 6803. J. Appl. Phycol. 2020, 32, 2889–2902. [Google Scholar] [CrossRef]
  25. Choi, S.Y.; Lee, H.J.; Choi, J.; Kim, J.; Sim, S.J.; Um, Y.; Kim, Y.; Lee, T.S.; Keasling, J.D.; Woo, H.M. Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria. Biotechnol. Biofuels 2016, 9, 202. [Google Scholar] [CrossRef]
  26. Rautela, A.; Yadav, I.; Gangwar, A.; Chatterjeee, R.; Kumar, S. Photosynthetic production of α-farnesene by engineered Synechococcus elongatus UTEX 2973 from carbon dioxide. Bioresour. Technol. 2024, 396, 130432. [Google Scholar] [CrossRef] [PubMed]
  27. Wang, X.; Liu, W.; Xin, C.; Zheng, Y.; Cheng, Y.; Sun, S.; Li, R.; Zhu, X.-G.; Dai, S.Y.; Rentzepis, P.M.; et al. Enhanced limonene production in cyanobacteria reveals photosynthesis limitations. Proc. Natl. Acad. Sci. USA 2016, 113, 14225–14230. [Google Scholar] [CrossRef]
  28. Lin, P.-C.; Saha, R.; Zhang, F.; Pakrasi, H.B. Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocystis sp. PCC 6803. Sci. Rep. 2017, 7, 17503. [Google Scholar] [CrossRef]
  29. Betterle, N.; Melis, A. Photosynthetic generation of heterologous terpenoids in cyanobacteria. Biotechnol. Bioeng. 2019, 116, 2041–2051. [Google Scholar] [CrossRef]
  30. Li, M.; Long, B.; Dai, S.Y.; Golden, J.W.; Wang, X.; Yuan, J. Altered Carbon Partitioning Enhances CO2 to Terpene Conversion in Cyanobacteria. BioDesign Res. 2022, 2022, 9897425. [Google Scholar] [CrossRef]
  31. Luan, G.; Zhang, S.; Wang, M.; Lu, X. Progress and perspective on cyanobacterial glycogen metabolism engineering. Biotechnol. Adv. 2019, 37, 771–786. [Google Scholar] [CrossRef] [PubMed]
  32. Koch, M.; Forchhammer, K. Polyhydroxybutyrate: A Useful Product of Chlorotic Cyanobacteria. Microbiol. Physiol. 2021, 31, 67–77. [Google Scholar] [CrossRef] [PubMed]
  33. Melis, A. Carbon partitioning in photosynthesis. Curr. Opin. Chem. Biol. 2013, 17, 453–456. [Google Scholar] [CrossRef] [PubMed]
  34. Taroncher-Oldenbourg, G.; Nishina, K.; Stephanopulos, G. Identification and Analysis of the Polyhydroxyalkanoate-Specific β-Ketothiolase and Acetoacetyl Coenzyme A Reductase Genes in the Cyanobacterium Synechocystis sp. Strain PCC6803. Appl. Environ. Microbiol. 2000, 66, 4440–4448. [Google Scholar] [CrossRef] [PubMed]
  35. Khetkorn, W.; Incharoensakdi, A.; Lindblad, P.; Jantaro, S. Enhancement of poly-3-hydroxybutyrate production in Synechocystis sp. PCC 6803 by overexpression of its native biosynthetic genes. Bioresour. Technol. 2016, 214, 761–768. [Google Scholar] [CrossRef] [PubMed]
  36. Velmurugan, R.; Incharroensakdi, A. Disruption of Polyhydroxybutyrate Synthesis Redirects Carbon Flow towards Glycogen Synthesis in Synechocystis sp. PCC 6803 Overexpressing glgC/glgA. Plant Cell Physiol. 2018, 59, 2020–2029. [Google Scholar] [CrossRef]
  37. Zhang, S.; Liu, Y.; Bryant, D.A. Metabolic engineering of Synechococcus sp. PCC 7002 to produce poly-3-hydroxybutyrate and poly-3-hydroxybutyrate-co-4-hydroxybutyrate. Metab. Eng. 2015, 32, 174–183. [Google Scholar] [CrossRef] [PubMed]
  38. Pagels, F.; Vasconcelos, V.; Guedes, A.C. Carotenoids from Cyanobacteria: Biotechnological Potential and Optimization Strategies. Biomolecules 2021, 11, 735. [Google Scholar] [CrossRef] [PubMed]
  39. Lagarde, D.; Vermaas, W. The zeaxanthin biosynthesis enzyme beta-carotene hydroxylase is involved in myxoxanthophyll synthesis in Synechocystis sp. PCC 6803. FEBS Lett. 1999, 454, 247–251. [Google Scholar] [CrossRef]
  40. Schafer, L.; Vioque, A.; Sandmann, G. Functional in situ evaluation of photosynthesis-protecting carotenoids in mutants of the cyanobacterium Synechocystis PCC6803. J. Photochem. Photobiol. B Biol. 2005, 78, 195–201. [Google Scholar] [CrossRef]
  41. Clark, R.L.; Gordon, G.C.; Bennett, N.R.; Lyu, H.; Root, T.W.; Pfleger, B.F. High-CO2 Requirement as a Mechanism for the Containment of Genetically Modified Cyanobacteria. ACS Synth. Biol. 2018, 7, 384–391. [Google Scholar] [CrossRef] [PubMed]
  42. Lee, H.J.; Choi, J.I.; Woo, H.M. Biocontainment of Engineered Synechococcus elongatus PCC 7942 for Photosynthetic Production of α-Farnesene from CO2. J. Agric. Food Chem. 2021, 69, 698–703. [Google Scholar] [CrossRef] [PubMed]
  43. Garcia-Alles, L.; Root, K.; Maveyraud, L.; Aubry, N.; Lesniewska, E.; Mourey, L.; Zenobi, R.; Truan, G. Occurrence and stability of hetero-hexamer associations formed by β-carboxysome CcmK shell components. PLoS ONE 2019, 14, e0223877. [Google Scholar] [CrossRef] [PubMed]
  44. Sommer, M.; Sutter, M.; Gupta, S.; Kirst, H.; Turmo, A.; Lechno-Yossef, S.; Burton, R.L.; Saechao, C.; Sloan, N.B.; Cheng, X.; et al. Heterohexamers formed by CcmK3 and CcmK4 increase the complexity of beta carboxysome shells. Plant Physiol. 2019, 179, 156–167. [Google Scholar] [CrossRef]
  45. Rae, B.D.; Long, B.M.; Badger, M.R.; Price, G.D. Structural determinants of the outer shell of β-carboxysomes in Synechococcus elongatus PCC 7942: Roles for CcmK2, K3-K4, CcmO, and CcmL. PLoS ONE 2012, 7, e43871. [Google Scholar] [CrossRef] [PubMed]
  46. Zhang, S.; Laborde, S.M.; Frankel, L.K.; Bricker, T.M. Four Novel Genes Required for Optimal Photoautotrophic Growth of the Cyanobacterium Synechocystis sp. Strain PCC 6803 Identified by In Vitro Transposon Mutagenesis. J. Bacteriol. 2004, 186, 875–879. [Google Scholar] [CrossRef] [PubMed]
  47. Stanier, R.Y.; Kunisawa, R.; Mandel, M.; Cohen-Bazire, G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol. Rev. 1971, 35, 171–205. [Google Scholar] [CrossRef]
  48. Stevens, S.E.; Pat Paterson, C.O.; Myers, J. The production of hydrogen peroxide by blue-green algae: A survey. J. Phycol. 1973, 9, 427–430. [Google Scholar] [CrossRef]
  49. Lee, H.J.; Lee, J.; Lee, S.-M.; Um, Y.; Kim, Y.; Sim, S.J.; Choi, J.-I.; Woo, H.M. Direct Conversion of CO2 to α-Farnesene Using Metabolically Engineered Synechococcus elongatus PCC 7942. J. Agric. Food Chem. 2017, 60, 10424–10428. [Google Scholar] [CrossRef]
  50. Mermet-Bouvier, P.; Chauvat, F. A conditional expression vector for the cyanobacteria Synechocystis sp. strains PCC6803 and PCC6714 or Synechococcus sp. strains PCC7942 and PCC63013. Curr. Microbiol. 1994, 28, 145–148. [Google Scholar] [CrossRef]
Ijms 25 03827 g001
Figure 1. Schematic representation of the metabolic pathway and key compounds involved in the synthesis of terpenes from CO2. Abbreviations: CA carbonic anhydrase; CBBC: Calvin–Benson–Bassham cycle (consuming 1 NADPH, 1 ATP); CrtE: geranylgeranyl pyrophosphate synthase; DMAPP: dimethylallyl pyrophosphate; DXP: 1-deoxy-D-xylulose-5-phosphate; Fed: ferredoxin; FPP: farnesyl pyrophosphate; FS: farnesene synthase; GAPDH: glyceraldehyde-3P-dehydrogenase; GPP: geranyl pyrophosphate; GPP: geranyl pyrophosphate; GGPP: geranylgeranyl pyrophosphate; G3P: glyceraldehyde-3-phosphate; 3PGA: 3-phosphoglycerate; IPP: isopentenyl pyrophosphate; IDI: isopentenyl-diphosphate isomerase; LS: limonene synthase; MEP: methylerythritol 4-phosphate (it consumes 2 NADPH, 1 ATP and 1 CTP); phaA: acetyl-CoA acetyltransferase; phaB: Acetoacetyl-CoA reductase; phaC: PHB synthase; PRK: phosphoribulokinase; Rbc: ribulose biphosphate carboxylase (RubisCO); RuBP: ribulose-1,5-bisphosphate. The genes manipulated in this study encode the CrtE, CrtR, CruF, PhaA, PhaB, PRK and RubisCO enzymes, as well as the CcmK3 and CcmK4 carboxysome shell proteins.
Figure 1. Schematic representation of the metabolic pathway and key compounds involved in the synthesis of terpenes from CO2. Abbreviations: CA carbonic anhydrase; CBBC: Calvin–Benson–Bassham cycle (consuming 1 NADPH, 1 ATP); CrtE: geranylgeranyl pyrophosphate synthase; DMAPP: dimethylallyl pyrophosphate; DXP: 1-deoxy-D-xylulose-5-phosphate; Fed: ferredoxin; FPP: farnesyl pyrophosphate; FS: farnesene synthase; GAPDH: glyceraldehyde-3P-dehydrogenase; GPP: geranyl pyrophosphate; GPP: geranyl pyrophosphate; GGPP: geranylgeranyl pyrophosphate; G3P: glyceraldehyde-3-phosphate; 3PGA: 3-phosphoglycerate; IPP: isopentenyl pyrophosphate; IDI: isopentenyl-diphosphate isomerase; LS: limonene synthase; MEP: methylerythritol 4-phosphate (it consumes 2 NADPH, 1 ATP and 1 CTP); phaA: acetyl-CoA acetyltransferase; phaB: Acetoacetyl-CoA reductase; phaC: PHB synthase; PRK: phosphoribulokinase; Rbc: ribulose biphosphate carboxylase (RubisCO); RuBP: ribulose-1,5-bisphosphate. The genes manipulated in this study encode the CrtE, CrtR, CruF, PhaA, PhaB, PRK and RubisCO enzymes, as well as the CcmK3 and CcmK4 carboxysome shell proteins.
Ijms 25 03827 g001
Ijms 25 03827 g002
Figure 2. Simultaneous analysis of growth (A) and farnesene production (B) of the Synechocystis PCC 6803 strains harboring the strongly expressed genes encoding farnesene-synthase (FS) in a neutral chromosomal site (chrFS) and the RubisCO (rbc) genes, in a pC-derived replicative plasmid (pCrbc). These plasmids encode the RubisCO enzymes from the distantly related unicellular cyanobacteria Cyanothece PCC 7425 (pCrbc7425), Synechococcus PCC 7002 (pCrbc7002), Synechococcus PCC 7942 (pCrbc7942) and Synechocystis PCC 6803 (pCrbc6803). Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Figure 2. Simultaneous analysis of growth (A) and farnesene production (B) of the Synechocystis PCC 6803 strains harboring the strongly expressed genes encoding farnesene-synthase (FS) in a neutral chromosomal site (chrFS) and the RubisCO (rbc) genes, in a pC-derived replicative plasmid (pCrbc). These plasmids encode the RubisCO enzymes from the distantly related unicellular cyanobacteria Cyanothece PCC 7425 (pCrbc7425), Synechococcus PCC 7002 (pCrbc7002), Synechococcus PCC 7942 (pCrbc7942) and Synechocystis PCC 6803 (pCrbc6803). Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Ijms 25 03827 g002
Ijms 25 03827 g003
Figure 3. Simultaneous analysis of growth (A) and farnesene production (B) of the Synechocystis PCC 6803 strain harboring the strongly expressed genes encoding farnesene synthase (FS) and phoshoribulokinase (prk) in a neutral chromosomal site (chrFS) and a pC-derived replicative plasmid, respectively. This pCprk7425 plasmid encodes the PRK enzyme from Cyanothece PCC 7425, a unicellular cyanobacterium distantly related to both Synechocystis PCC 6803 and Synechococcus PCC 7002. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Figure 3. Simultaneous analysis of growth (A) and farnesene production (B) of the Synechocystis PCC 6803 strain harboring the strongly expressed genes encoding farnesene synthase (FS) and phoshoribulokinase (prk) in a neutral chromosomal site (chrFS) and a pC-derived replicative plasmid, respectively. This pCprk7425 plasmid encodes the PRK enzyme from Cyanothece PCC 7425, a unicellular cyanobacterium distantly related to both Synechocystis PCC 6803 and Synechococcus PCC 7002. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Ijms 25 03827 g003
Ijms 25 03827 g004
Figure 4. Simultaneous analysis of growth (A) and limonene production (B) of the Synechococcus PCC 7002 strains harboring the strongly expressed limonene-synthase gene (LS) in a neutral chromosomal site (chrLS) and a pC-derived plasmid expressing either the phoshoribulokinase gene from Cyanothece PCC 7425 (pCprk7425) or the RubisCO genes from Cyanothece PCC 7425 (pCrbc7425), Synechococcus PCC 7002 (pCrbc7002), Synechococcus PCC 7942 (pCrbc7942) or Synechocystis PCC 6803 (pCrbc6803). Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel A.
Figure 4. Simultaneous analysis of growth (A) and limonene production (B) of the Synechococcus PCC 7002 strains harboring the strongly expressed limonene-synthase gene (LS) in a neutral chromosomal site (chrLS) and a pC-derived plasmid expressing either the phoshoribulokinase gene from Cyanothece PCC 7425 (pCprk7425) or the RubisCO genes from Cyanothece PCC 7425 (pCrbc7425), Synechococcus PCC 7002 (pCrbc7002), Synechococcus PCC 7942 (pCrbc7942) or Synechocystis PCC 6803 (pCrbc6803). Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel A.
Ijms 25 03827 g004
Ijms 25 03827 g005
Figure 5. Simultaneous analysis of growth (A,C) and terpene production (B,D) of Synechocystis PCC 6803 strains harboring a pC-derived plasmid strongly expressing either the farnesene synthase gene (pCFS; (A,B)) alone or transcriptionally fused to the crtE gene from either Synechocystis PCC 6803 (pCFScrtE6803) or Synechococcus PCC 7002 (pCFScrtE7002), or the limonene synthase gene (pCLS; (C,D)) alone or transcriptionally fused to a crtE gene (pCLScrtE6803 or pCLScrtE7002). Several clones were studied, as indicated by C1-C8. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panels (A,C). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Figure 5. Simultaneous analysis of growth (A,C) and terpene production (B,D) of Synechocystis PCC 6803 strains harboring a pC-derived plasmid strongly expressing either the farnesene synthase gene (pCFS; (A,B)) alone or transcriptionally fused to the crtE gene from either Synechocystis PCC 6803 (pCFScrtE6803) or Synechococcus PCC 7002 (pCFScrtE7002), or the limonene synthase gene (pCLS; (C,D)) alone or transcriptionally fused to a crtE gene (pCLScrtE6803 or pCLScrtE7002). Several clones were studied, as indicated by C1-C8. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panels (A,C). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Ijms 25 03827 g005
Ijms 25 03827 g006
Figure 6. Simultaneous analysis of growth (A) and farnesene production (B) of Synechococcus PCC 7002 strains harboring a pC-derived plasmid strongly expressing either the farnesene synthase gene (pCFS) alone or transcriptionally fused to the crtE gene from either Synechocystis PCC 6803 (pCFScrtE6803) or Synechococcus PCC 7002 (pCFScrtE7002). Three clones were studied, as indicated by C3, C6 and C9. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A), except in one case. The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Figure 6. Simultaneous analysis of growth (A) and farnesene production (B) of Synechococcus PCC 7002 strains harboring a pC-derived plasmid strongly expressing either the farnesene synthase gene (pCFS) alone or transcriptionally fused to the crtE gene from either Synechocystis PCC 6803 (pCFScrtE6803) or Synechococcus PCC 7002 (pCFScrtE7002). Three clones were studied, as indicated by C3, C6 and C9. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A), except in one case. The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Ijms 25 03827 g006
Ijms 25 03827 g007
Figure 7. Simultaneous analysis of growth (A,C) and terpene production (B,D) of Synechocystis PCC 6803 strains WT or ΔphaAB mutant harboring a pC-derived plasmid strongly expressing either the limonene synthase gene (pCLS; (A,B)) or the farnesene synthase gene (pCFS; (C,D)). Several clones were studied, as indicated by C1–C5. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 17 days. Error bars represent standard deviation from biological triplicates. Most of them are too small to be visible in panels (A,C).
Figure 7. Simultaneous analysis of growth (A,C) and terpene production (B,D) of Synechocystis PCC 6803 strains WT or ΔphaAB mutant harboring a pC-derived plasmid strongly expressing either the limonene synthase gene (pCLS; (A,B)) or the farnesene synthase gene (pCFS; (C,D)). Several clones were studied, as indicated by C1–C5. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 17 days. Error bars represent standard deviation from biological triplicates. Most of them are too small to be visible in panels (A,C).
Ijms 25 03827 g007
Ijms 25 03827 g008
Figure 8. Simultaneous analysis of growth (A) and farnesene production (B) of Synechocystis PCC 6803 WT strain and two clones (1 and 2) of each of the ΔcrtR and ΔcruF mutants harboring the farnesene synthase production pCFS plasmid. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay farnesene production (measured at day 10). Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Figure 8. Simultaneous analysis of growth (A) and farnesene production (B) of Synechocystis PCC 6803 WT strain and two clones (1 and 2) of each of the ΔcrtR and ΔcruF mutants harboring the farnesene synthase production pCFS plasmid. Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay farnesene production (measured at day 10). Error bars represent standard deviation from biological triplicates. They are too small to be visible in panel (A). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Ijms 25 03827 g008
Ijms 25 03827 g009
Figure 9. Simultaneous analysis of growth (A,C) and terpene production (B,D) of Synechocystis PCC 6803 strains WT or ΔccmK3K4 mutant harboring a pC-derived plasmid strongly expressing either the limonene synthase gene (pCLS; (A,B)) or the farnesene synthase gene (pCFS; (C,D)). Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 17 or 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panels (A,C). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Figure 9. Simultaneous analysis of growth (A,C) and terpene production (B,D) of Synechocystis PCC 6803 strains WT or ΔccmK3K4 mutant harboring a pC-derived plasmid strongly expressing either the limonene synthase gene (pCLS; (A,B)) or the farnesene synthase gene (pCFS; (C,D)). Cells were grown under standard photoautotrophic conditions in the presence of a dodecane overlay (20% v/v) to assay terpene production for 17 or 21 days. Error bars represent standard deviation from biological triplicates. They are too small to be visible in panels (A,C). The hooks ⊏ indicate a significant difference between the two compared experiments (t-test, p < 0.05, symbolized by *).
Ijms 25 03827 g009
Table 1. Summary of the terpene producing strains engineered/studied in this study.
Table 1. Summary of the terpene producing strains engineered/studied in this study.
HostStrain DesignationRelevant FeaturesTerpene
and Figure		Yield
mg/L
S.6803ChrFSExpression of the farnesene synthase gene (FS) cloned in a neutral chromosomal siteFarnesene
Figure 2		2.7
ChrFS + pCrbc6803ChrFS strain expressing the S.6803 RubisCO genes from the pC plasmid 2.5
ChrFS + pCrbc7002ChrFS expressing the S.7002 RubisCO genes from pC 5.2
ChrFS + pCrbc7942ChrFS expressing the S.7942 RubisCO genes from pC 2.5
ChrFS + pCrbcC7425ChrFS expressing the C.7425 RubisCO genes from pC 4.2
S.6803ChrFSExpression of FS Farnesene
Figure 3		2.7
ChrFS + pCprk7425ChrFS expressing the C.7425 prk gene from pC 10.2
S.7002ChrLSExpression of the limonene synthase gene (LS) from a neutral chromosomal siteLimonene
Figure 4		0.8
ChrLS + pCrbc6803ChrLS expressing the S.6803 RubisCO genes from pC 0.9
ChrLS + pCrbc7002ChrLS expressing the S.7002 RubisCO genes from pC 0.8–0.9
ChrLS + pCrbc7942ChrLS expressing the S.7942 RubisCO genes from pC 0.8–0.9
ChrLS + pCrbcC7425ChrLS expressing the C.7425 RubisCO genes from pC 0.8–0.9
ChrLS + pCprk7425ChrLS expressing the C.7425 prk gene from pC 1.0
S.6803pCFSExpression of FS from pCFarnesene
Figure 5		12.0
pCFScrtE6803Expression of FS and the S.6803 crtE gene from pC ≤3.0
pCFScrtE7002Expression of FS and the S.7002 crtE gene from pC ≤3.0
pCLSExpression of LS from pCLimonene
Figure 5		0.55
pCLScrtE6803Expression of LS and the S.6803 crtE gene from pC 0.05
pCLScrtE7002Expression of LS and S.7002 crtE from pC 0.2
S.7002pCFSExpression of FS from pCFarnesene
Figure 6		2.2
pCFScrtE6803Expression of LS and S.6803 crtE from pC 3.8
pCFScrtE7002Expression of LS and S.7002 crtE from pC 1.8
S.6803pCLSExpression of LS from pCLimonene
Figure 7		0.8
ΔphAB pCLS Expression of LS from pC and deletion of the phaAB genes 0.9–1.2
pCFSExpression of FS from pC 22.0
ΔphAB pCFS Expression of FS from pC and deletion of phaAB 20–25
S.6803pCFSExpression of FS from pCFarnesene
Figure 8		12.5
ΔcrtR pCFSExpression of FS from pC and deletion of the crtR gene 3.7
ΔcruF pCFSExpression of FS from pC and deletion of the cruF gene 4.5
S.6803pCFSExpression of FS from pCFarnesene
Figure 9		7.0
ΔccmK3K4 pCFS Expression of FS from pC and deletion of the ccmK3K4 genes 5.5
pCLSExpression of LS from pCLimonene
Figure 9		0.35
ΔccmK3K4 pCLSExpression of the LS gene from pC and deletion of ccmK3K4 0.45
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Vincent, M.; Blanc-Garin, V.; Chenebault, C.; Cirimele, M.; Farci, S.; Garcia-Alles, L.F.; Cassier-Chauvat, C.; Chauvat, F. Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002. Int. J. Mol. Sci. 2024, 25, 3827. https://doi.org/10.3390/ijms25073827

AMA Style

Vincent M, Blanc-Garin V, Chenebault C, Cirimele M, Farci S, Garcia-Alles LF, Cassier-Chauvat C, Chauvat F. Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002. International Journal of Molecular Sciences. 2024; 25(7):3827. https://doi.org/10.3390/ijms25073827

Chicago/Turabian Style

Vincent, Marine, Victoire Blanc-Garin, Célia Chenebault, Mattia Cirimele, Sandrine Farci, Luis Fernando Garcia-Alles, Corinne Cassier-Chauvat, and Franck Chauvat. 2024. "Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002" International Journal of Molecular Sciences 25, no. 7: 3827. https://doi.org/10.3390/ijms25073827

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop