The Dual Role of Neutrophil Extracellular Traps (NETs) in Sepsis and Ischemia-Reperfusion Injury: Comparative Analysis across Murine Models
Abstract
:1. Introduction
2. Results
2.1. Survival in the Septic Models Depended on the Modulation of Neutrophil Extracellular Trap Levels
2.2. Plasma Levels of Cell-Free DNA and DNase1
2.3. Tissue Expression of DNase1 and DNase1L3
2.4. Oxidative Stress and Antioxidative Capacity
3. Discussion
Limitations
4. Conclusions
5. Materials and Methods
5.1. Study Design
5.2. Animal Procedures
5.2.1. Circulating Free Deoxyribonucleic Acid (cfDNA)
5.2.2. Deoxyribonuclease I (DNase1)
5.2.3. Neutrophil Elastase (NE) Enzyme-Linked Immunosorbent Assay (ELISA)
5.2.4. Immunofluorescence Staining
5.2.5. Glutathione Peroxidase (GPx) Assay
5.2.6. Malondialdehyde (MDA) Assay
5.3. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kiwit, A.; Lu, Y.; Lenz, M.; Knopf, J.; Mohr, C.; Ledermann, Y.; Klinke-Petrowsky, M.; Pagerols Raluy, L.; Reinshagen, K.; Herrmann, M.; et al. The Dual Role of Neutrophil Extracellular Traps (NETs) in Sepsis and Ischemia-Reperfusion Injury: Comparative Analysis across Murine Models. Int. J. Mol. Sci. 2024, 25, 3787. https://doi.org/10.3390/ijms25073787
Kiwit A, Lu Y, Lenz M, Knopf J, Mohr C, Ledermann Y, Klinke-Petrowsky M, Pagerols Raluy L, Reinshagen K, Herrmann M, et al. The Dual Role of Neutrophil Extracellular Traps (NETs) in Sepsis and Ischemia-Reperfusion Injury: Comparative Analysis across Murine Models. International Journal of Molecular Sciences. 2024; 25(7):3787. https://doi.org/10.3390/ijms25073787
Chicago/Turabian StyleKiwit, Antonia, Yuqing Lu, Moritz Lenz, Jasmin Knopf, Christoph Mohr, Yannick Ledermann, Michaela Klinke-Petrowsky, Laia Pagerols Raluy, Konrad Reinshagen, Martin Herrmann, and et al. 2024. "The Dual Role of Neutrophil Extracellular Traps (NETs) in Sepsis and Ischemia-Reperfusion Injury: Comparative Analysis across Murine Models" International Journal of Molecular Sciences 25, no. 7: 3787. https://doi.org/10.3390/ijms25073787