Identification of Dhx15 as a Major Regulator of Liver Development, Regeneration, and Tumor Growth in Zebrafish and Mice
Abstract
:1. Introduction
2. Results
2.1. Impaired Liver Development in Dhx15-Deficient Zebrafish Embryos
2.2. Altered Liver Vasculature in Dhx15-Partially-Deficient Mice
2.3. Dhx15 Partial Gene Deficiency Decreases the Regenerative Capacity of the Liver in Mice
2.4. Dhx15 Deficiency Alters Glucose Metabolism
2.5. Dhx15-Related Vascular Alterations Derive in Less Hepatic Tumor Nodule Events in an HCC Mouse Model
2.6. AUMsilence ASO Mediated Dhx15 Silencing in Mice Reduces Primary Tumor Volume in an HCC Mouse Model
3. Discussion
4. Materials and Methods
4.1. Mouse-Induced Tumor Model
4.2. In Vivo Knockdown Experiments
4.3. Patients
4.4. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Portolés, I.; Ribera, J.; Fernandez-Galán, E.; Lecue, E.; Casals, G.; Melgar-Lesmes, P.; Fernández-Varo, G.; Boix, L.; Sanduzzi, M.; Aishwarya, V.; et al. Identification of Dhx15 as a Major Regulator of Liver Development, Regeneration, and Tumor Growth in Zebrafish and Mice. Int. J. Mol. Sci. 2024, 25, 3716. https://doi.org/10.3390/ijms25073716
Portolés I, Ribera J, Fernandez-Galán E, Lecue E, Casals G, Melgar-Lesmes P, Fernández-Varo G, Boix L, Sanduzzi M, Aishwarya V, et al. Identification of Dhx15 as a Major Regulator of Liver Development, Regeneration, and Tumor Growth in Zebrafish and Mice. International Journal of Molecular Sciences. 2024; 25(7):3716. https://doi.org/10.3390/ijms25073716
Chicago/Turabian StylePortolés, Irene, Jordi Ribera, Esther Fernandez-Galán, Elena Lecue, Gregori Casals, Pedro Melgar-Lesmes, Guillermo Fernández-Varo, Loreto Boix, Marco Sanduzzi, Veenu Aishwarya, and et al. 2024. "Identification of Dhx15 as a Major Regulator of Liver Development, Regeneration, and Tumor Growth in Zebrafish and Mice" International Journal of Molecular Sciences 25, no. 7: 3716. https://doi.org/10.3390/ijms25073716