Overexpression of GmPAP4 Enhances Symbiotic Nitrogen Fixation and Seed Yield in Soybean under Phosphorus-Deficient Condition
Abstract
:1. Introduction
2. Results
2.1. GmPAP4 Was Preferentially Expressed in Infection Zones of Nodules in Soybean
2.2. GmPAP4 Was Highly Induced by Low Phosphorus Stress in Soybean Nodules
2.3. GmPAP4 Expression Was Positively Associated with Nodulation and Nitrogen Fixation under LP Stress
2.4. Overexpression of GmPAP4-Enhanced Soybean Plant Growth under P-Deficient Conditions
2.5. Overexpression of GmPAP4 Enhanced Soybean Yield under P-Deficient Conditions
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Plant mRNA Isolation and Quantitative Real-Time PCR (qRT-PCR)
4.3. Histochemical GUS Staining Analysis
4.4. Generation of Stable Transgenic Soybean Plants
4.5. RNA In Situ Hybridization Analysis
4.6. Toluidine Blue Staining
4.7. Measurement of N and P Contents
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | Sequence (5′ to 3′) |
---|---|
GmPAP4-RT-F1 | GAGCCGTTTGCGAGTACAAG |
GmPAP4-RT-R1 | TCTGCATAGGAGCCAAGCAT |
GmActin11-F | ATCTTGACTGAGCGTGGTTATTCC |
GmActin11-R | GCTGGTCCTGGCTGTCTCC |
Bar-F | CCATCGTCAACCACTACATCGAGACA |
Bar-R | CTTCAGCAGGTGGGTGTAGAGCGT |
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Sun, X.; Zhang, H.; Yang, Z.; Xing, X.; Fu, Z.; Li, X.; Kong, Y.; Li, W.; Du, H.; Zhang, C. Overexpression of GmPAP4 Enhances Symbiotic Nitrogen Fixation and Seed Yield in Soybean under Phosphorus-Deficient Condition. Int. J. Mol. Sci. 2024, 25, 3649. https://doi.org/10.3390/ijms25073649
Sun X, Zhang H, Yang Z, Xing X, Fu Z, Li X, Kong Y, Li W, Du H, Zhang C. Overexpression of GmPAP4 Enhances Symbiotic Nitrogen Fixation and Seed Yield in Soybean under Phosphorus-Deficient Condition. International Journal of Molecular Sciences. 2024; 25(7):3649. https://doi.org/10.3390/ijms25073649
Chicago/Turabian StyleSun, Xi, Huantao Zhang, Zhanwu Yang, Xinzhu Xing, Zhao Fu, Xihuan Li, Youbin Kong, Wenlong Li, Hui Du, and Caiying Zhang. 2024. "Overexpression of GmPAP4 Enhances Symbiotic Nitrogen Fixation and Seed Yield in Soybean under Phosphorus-Deficient Condition" International Journal of Molecular Sciences 25, no. 7: 3649. https://doi.org/10.3390/ijms25073649