CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3
Abstract
:1. Introduction
2. Results
2.1. CircMTA2 Is Upregulated in the GC Tissue and Cells
2.2. CircMTA2 Promotes the Growth and Aggressiveness of GC
2.3. CircMTA2 Stabilities MTA2 Protein by Inhibiting Its Ubiquitination
2.4. CircMTA2 Promotes Growth and Aggressiveness of GC by Upregulating MTA2 Expression
2.5. CircMTA2 Promotes MTA2 Expression via Interacting with UCHL3 in GC Cells
2.6. Exosomal circMTA2 Derived from GC Cells Enhances Cancer Cell Progression
3. Materials and Methods
3.1. Cell Lines and Treatment
3.2. Patient Tissue Samples
3.3. Fluorescence In Situ Hybridization (FISH)
3.4. RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)
3.5. RNase R and Actinomycin D Assays
3.6. RNA-Protein Immunoprecipitation (RIP) Assay
3.7. RNA Pulldown Assay
3.8. Western Blotting Analysis
3.9. CCK-8 Assay
3.10. Colony Formation Assay
3.11. 5-Ethynyl-20-Deoxyuridine (EdU) Analysis
3.12. Terminal Deoxynucleotidyl Transferase (TdT)-Mediated dUTP Biotin Nick End Labeling (TUNEL) Staining
3.13. Transwell Assay
3.14. Xenograft Assay
3.15. Immunohistochemical (IHC) Assay
3.16. Exosome Isolation and Characterization
4. Statistical Analysis
5. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xie, G.; Lei, B.; Yin, Z.; Xu, F.; Liu, X. CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3. Int. J. Mol. Sci. 2024, 25, 2817. https://doi.org/10.3390/ijms25052817
Xie G, Lei B, Yin Z, Xu F, Liu X. CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3. International Journal of Molecular Sciences. 2024; 25(5):2817. https://doi.org/10.3390/ijms25052817
Chicago/Turabian StyleXie, Gengchen, Bo Lei, Zhijie Yin, Fei Xu, and Xinghua Liu. 2024. "CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3" International Journal of Molecular Sciences 25, no. 5: 2817. https://doi.org/10.3390/ijms25052817