Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling
Abstract
:1. Introduction
2. Results
2.1. Immortalized MSCs (iMSCs) Demonstrate Higher Telomerase Activity and Proliferation Potential Together with Delayed Replicative Senescence
2.2. Immortalized MSCs (iMSCs) Retain MSC-Specific Immunophenotype and Differentiation Potential
2.3. Hormonal Sensitivity of iMSCs Does Not Differ from Primary MSCs
3. Discussion
4. Materials and Methods
4.1. Cell Cultures
4.2. hTERT-Encoding Lentivirus Construction
4.3. Human TERT Expression and Activity
4.4. MSC Immunophenotyping
4.5. MSC Proliferation
4.6. MSC Hormonal Sensitivity
4.7. MCS Multilineage Differentiation Assays
4.7.1. Osteogenic Differentiation
4.7.2. Adipogenic Differentiation
4.7.3. Chondrogenic Differentiation
4.8. Senescence-Associated Beta-Galactosidase Staining
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primak, A.; Kalinina, N.; Skryabina, M.; Usachev, V.; Chechekhin, V.; Vigovskiy, M.; Chechekhina, E.; Voloshin, N.; Kulebyakin, K.; Kulebyakina, M.; et al. Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling. Int. J. Mol. Sci. 2024, 25, 2421. https://doi.org/10.3390/ijms25042421
Primak A, Kalinina N, Skryabina M, Usachev V, Chechekhin V, Vigovskiy M, Chechekhina E, Voloshin N, Kulebyakin K, Kulebyakina M, et al. Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling. International Journal of Molecular Sciences. 2024; 25(4):2421. https://doi.org/10.3390/ijms25042421
Chicago/Turabian StylePrimak, Alexandra, Natalia Kalinina, Mariya Skryabina, Vladimir Usachev, Vadim Chechekhin, Maksim Vigovskiy, Elizaveta Chechekhina, Nikita Voloshin, Konstantin Kulebyakin, Maria Kulebyakina, and et al. 2024. "Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling" International Journal of Molecular Sciences 25, no. 4: 2421. https://doi.org/10.3390/ijms25042421