Carnosic Acid against Lung Cancer: Induction of Autophagy and Activation of Sestrin-2/LKB1/AMPK Signalling
Abstract
:1. Introduction
2. Results
2.1. Carnosic Acid Inhibits Proliferation and Survival of NSCLC Cells
2.2. Carnosic Acid Induces Apoptosis of H1299 NSCLC Cells
2.3. Carnosic Acid Activates Sestrin-2/LKB1/AMPK Signalling in H1299 NSCLC Cells
2.4. Carnosic Acid Activates Autophagy Signalling in H1299 NSCLC Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Crystal Violet Proliferation Assay
4.4. [3H]-Thymidine Incorporation Assay
4.5. Clonogenic Survival Assay
4.6. SDS-PAGE and Western Blotting
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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O’Neill, E.J.; Sze, N.S.K.; MacPherson, R.E.K.; Tsiani, E. Carnosic Acid against Lung Cancer: Induction of Autophagy and Activation of Sestrin-2/LKB1/AMPK Signalling. Int. J. Mol. Sci. 2024, 25, 1950. https://doi.org/10.3390/ijms25041950
O’Neill EJ, Sze NSK, MacPherson REK, Tsiani E. Carnosic Acid against Lung Cancer: Induction of Autophagy and Activation of Sestrin-2/LKB1/AMPK Signalling. International Journal of Molecular Sciences. 2024; 25(4):1950. https://doi.org/10.3390/ijms25041950
Chicago/Turabian StyleO’Neill, Eric J., Newman Siu Kwan Sze, Rebecca E. K. MacPherson, and Evangelia Tsiani. 2024. "Carnosic Acid against Lung Cancer: Induction of Autophagy and Activation of Sestrin-2/LKB1/AMPK Signalling" International Journal of Molecular Sciences 25, no. 4: 1950. https://doi.org/10.3390/ijms25041950