A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis
Abstract
:1. Introduction
2. Results
2.1. SnRNA-Seq Identified Distinct Cell Populations in Longissimus Dorsi Muscle at Two Developmental Stages
2.2. Clustering Analysis Identified Subgroups in the Subpopulation of Myonuclei Cells
2.3. Analysis of the Heterogeneity of the FAP Subpopulation via Unsupervised Clustering
2.4. Genes Commonly Expressed During Two Stages of Adipocyte Development
2.5. Pseudotime Analysis Revealed Key Progenitor Cell Characteristics and Gene Sets of FAPs
3. Discussion
4. Materials and Methods
4.1. Animals and Samples
4.2. Library Construction and Sequencing
4.3. SnRNA-Seq Bioinformatics Analysis
4.4. Single-Cell Trajectory Analysis
4.5. Pathway Enrichment Analysis
4.6. Cell Culture, Interference, and Induction of Differentiation
4.7. RT-qPCR and BODIPY, Oil-Red-O Staining
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Estimated Number of Nuclei | Mean Reads Per Nuclei | Median Genes Per Nuclei | Total Genes Detected | Median UMI Counts Per Nuclei |
---|---|---|---|---|---|
D1_1 | 27,560 | 14,121 | 1150 | 22,556 | 2059 |
D1_2 | 27,677 | 13,028 | 1033 | 22,446 | 1748 |
D180_1 | 19,842 | 22,662 | 1194 | 22,547 | 2399 |
D180_2 | 3223 | 128,122 | 1391 | 21,808 | 2910 |
Groups | Nuclei Number after Filter | Median UMI Counts Per Nuclei After Filter | Median Genes Per Nuclei After Filter |
---|---|---|---|
D1 | 43,586 | 1716.5 | 994 |
D180 | 19,685 | 2163 | 1115 |
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Xu, Z.; Wu, J.; Li, Y.; Zhou, J.; Zhang, Y.; Qiao, M.; Feng, Y.; Sun, H.; Li, Z.; Li, L.; et al. A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis. Int. J. Mol. Sci. 2024, 25, 12935. https://doi.org/10.3390/ijms252312935
Xu Z, Wu J, Li Y, Zhou J, Zhang Y, Qiao M, Feng Y, Sun H, Li Z, Li L, et al. A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis. International Journal of Molecular Sciences. 2024; 25(23):12935. https://doi.org/10.3390/ijms252312935
Chicago/Turabian StyleXu, Zhong, Junjing Wu, Yujie Li, Jiawei Zhou, Yu Zhang, Mu Qiao, Yue Feng, Hua Sun, Zipeng Li, Lianghua Li, and et al. 2024. "A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis" International Journal of Molecular Sciences 25, no. 23: 12935. https://doi.org/10.3390/ijms252312935
APA StyleXu, Z., Wu, J., Li, Y., Zhou, J., Zhang, Y., Qiao, M., Feng, Y., Sun, H., Li, Z., Li, L., Oyelami, F. O., Peng, X., & Mei, S. (2024). A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis. International Journal of Molecular Sciences, 25(23), 12935. https://doi.org/10.3390/ijms252312935