Genome-Wide Analysis of R2R3-MYB Genes and Functional Characterization of SmMYB75 in Eggplant Fruit Implications for Crop Improvement and Nutritional Enhancement
Abstract
1. Introduction
2. Results
2.1. Identification and Physicochemical Property Analysis of the SmR2R3-MYB Genes
2.2. Phylogenetic Analysis and Classification of the SmR2R3-MYB Gene Family
2.3. Motif, Conserved Domain, and Structure Analyses of SmR2R3-MYBs
2.4. miRNAs That Target Regulate SmR2R3-MYB Genes
2.5. Chromosomal Distribution and Repeat Events of R2R3-MYB Genes in Eggplant Fruit
2.6. Comparative Synteny Analysis of R2R3-MYB Genes between Eggplants and Other Species
2.7. Expression Pattern of SmR2R3-MYBs under Diverse Treatments
2.8. Generation and Characterization of SmMYB75-OE Transgenic Eggplant Fruit
2.9. Anthocyanin-Targeted Metabolomic and Transcriptomic Analysis of Eggplant Flesh
2.10. Comprehensive Targeted Metabolic Profiling
3. Discussion
4. Materials and Methods
4.1. Identification and Classification of R2R3-MYB Genes in Eggplants
4.2. Phylogenetic Tree Analysis and Classification of R2R3-MYB Genes in Eggplants
4.3. Gene Structure, Conserved Domain, and Motif Analysis
4.4. miRNA-R2R3-MYBs Prediction
4.5. Chromosomal Localization, Gene Duplication, and Syntenic Analysis
4.6. Expression Profiles of Abiotic Stress-Responsive Results and qRT-PCR
4.7. Vector Construction and Plant Transformation
4.8. RNA-Seq Analysis
4.9. Extraction and Measurement of Total Anthocyanins
4.10. Metabolite Analysis
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shi, S.; Li, D.; Li, S.; Zhao, N.; Liao, J.; Ge, H.; Liu, Y.; Chen, H. Genome-Wide Analysis of R2R3-MYB Genes and Functional Characterization of SmMYB75 in Eggplant Fruit Implications for Crop Improvement and Nutritional Enhancement. Int. J. Mol. Sci. 2024, 25, 1163. https://doi.org/10.3390/ijms25021163
Shi S, Li D, Li S, Zhao N, Liao J, Ge H, Liu Y, Chen H. Genome-Wide Analysis of R2R3-MYB Genes and Functional Characterization of SmMYB75 in Eggplant Fruit Implications for Crop Improvement and Nutritional Enhancement. International Journal of Molecular Sciences. 2024; 25(2):1163. https://doi.org/10.3390/ijms25021163
Chicago/Turabian StyleShi, Suli, Dalu Li, Shaohang Li, Na Zhao, Jielei Liao, Haiyan Ge, Yang Liu, and Huoying Chen. 2024. "Genome-Wide Analysis of R2R3-MYB Genes and Functional Characterization of SmMYB75 in Eggplant Fruit Implications for Crop Improvement and Nutritional Enhancement" International Journal of Molecular Sciences 25, no. 2: 1163. https://doi.org/10.3390/ijms25021163
APA StyleShi, S., Li, D., Li, S., Zhao, N., Liao, J., Ge, H., Liu, Y., & Chen, H. (2024). Genome-Wide Analysis of R2R3-MYB Genes and Functional Characterization of SmMYB75 in Eggplant Fruit Implications for Crop Improvement and Nutritional Enhancement. International Journal of Molecular Sciences, 25(2), 1163. https://doi.org/10.3390/ijms25021163