Indoxyl Sulfate Inhibits Osteogenesis in Bone Marrow Mesenchymal Stem Cells through the AhR/Hes1 Pathway
Abstract
1. Introduction
2. Results
2.1. IS Suppresses the Mineralization of D1 Cells
2.2. IS Downregulates the Expression of Osteogenic Markers in D1 Cells
2.3. IS Activates AhR Signaling
2.4. IS Impairs Osteogenesis by Inducing AhR
2.5. IS Upregulates the Expression of Hes1 through AhR
2.6. Hes1 Downregulates the Expression of Runx2 and Bmp2
2.7. Hes1 Directly Binds to the Promoters of Runx2 and Bmp2
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. Alizarin Red S Staining
4.3. Quantitative Real-Time Polymerase Chain Reaction
4.4. Western Blotting
4.5. Measurement of Cytoplasmic and Nuclear AhR Concentrations
4.6. Immunofluorescence Staining
4.7. Ahr Knockdown
4.8. Luciferase Assay
4.9. Chromatin Immunoprecipitation
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hsieh, C.-W.; Chang, L.-H.; Wang, Y.-H.; Li, W.-T.; Chang, J.-K.; Chen, C.-H.; Ho, M.-L. Indoxyl Sulfate Inhibits Osteogenesis in Bone Marrow Mesenchymal Stem Cells through the AhR/Hes1 Pathway. Int. J. Mol. Sci. 2024, 25, 8770. https://doi.org/10.3390/ijms25168770
Hsieh C-W, Chang L-H, Wang Y-H, Li W-T, Chang J-K, Chen C-H, Ho M-L. Indoxyl Sulfate Inhibits Osteogenesis in Bone Marrow Mesenchymal Stem Cells through the AhR/Hes1 Pathway. International Journal of Molecular Sciences. 2024; 25(16):8770. https://doi.org/10.3390/ijms25168770
Chicago/Turabian StyleHsieh, Chin-Wen, Ling-Hua Chang, Yan-Hsiung Wang, Wei-Ting Li, Je-Ken Chang, Chung-Hwan Chen, and Mei-Ling Ho. 2024. "Indoxyl Sulfate Inhibits Osteogenesis in Bone Marrow Mesenchymal Stem Cells through the AhR/Hes1 Pathway" International Journal of Molecular Sciences 25, no. 16: 8770. https://doi.org/10.3390/ijms25168770
APA StyleHsieh, C.-W., Chang, L.-H., Wang, Y.-H., Li, W.-T., Chang, J.-K., Chen, C.-H., & Ho, M.-L. (2024). Indoxyl Sulfate Inhibits Osteogenesis in Bone Marrow Mesenchymal Stem Cells through the AhR/Hes1 Pathway. International Journal of Molecular Sciences, 25(16), 8770. https://doi.org/10.3390/ijms25168770