The Protective Role of Magnoliae Flos in Preventing Ovotoxicity and Managing Ovarian Function: An In Vitro and In Vivo Study
Abstract
:1. Introduction
2. Results
2.1. Effects of MFE on the Viability of CHO-K1 and COV434 Cells
2.2. Inhibitory Effects of MFE on VCD-Induced Reactive Oxygen Species (ROS) Generation
2.3. Inhibitory Effects of MFE on VCD-Induced Apoptosis
2.4. Effects of MFE on Akt Signaling Pathway in CHO-K1 Cells
2.5. Effects of MFE Administration on VCD-Induced Ovarian Failure in Mice
2.6. Effects of MFE Administration on Serum Hormone Levels and Hepatoxicity in the VCD-Induced Ovarian Failure Model
3. Discussion
4. Materials and Methods
4.1. Preparation of Magnoliae Flos Macerated Water Extract
4.2. HPLC Analysis of MFE
4.3. Cell Culture
4.4. Cell Viability Assay
4.5. Measurement of ROS
4.6. DAPI Nuclear Staining
4.7. Western Blot Analysis
4.8. Animal Experimental Design
4.9. Histopathological Analysis
4.10. Serum Biochemistry Analysis
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Kim, M.R.; Kim, D.-I.; Park, S.Y.; Kang, H.J.; Park, S.-D.; Lee, J.-H. The Protective Role of Magnoliae Flos in Preventing Ovotoxicity and Managing Ovarian Function: An In Vitro and In Vivo Study. Int. J. Mol. Sci. 2024, 25, 6456. https://doi.org/10.3390/ijms25126456
Kim MR, Kim D-I, Park SY, Kang HJ, Park S-D, Lee J-H. The Protective Role of Magnoliae Flos in Preventing Ovotoxicity and Managing Ovarian Function: An In Vitro and In Vivo Study. International Journal of Molecular Sciences. 2024; 25(12):6456. https://doi.org/10.3390/ijms25126456
Chicago/Turabian StyleKim, Mi Ra, Dong-Il Kim, Sung Yun Park, Hyo Jin Kang, Sun-Dong Park, and Ju-Hee Lee. 2024. "The Protective Role of Magnoliae Flos in Preventing Ovotoxicity and Managing Ovarian Function: An In Vitro and In Vivo Study" International Journal of Molecular Sciences 25, no. 12: 6456. https://doi.org/10.3390/ijms25126456